Genetic and Chemical Diversity of Edible Mushroom Pleurotus Species

Lin, Pei; Yan, Zheng‐Fei; Kook, MooChang; Li, Changtian; Yi, Tae–Hoo

doi:10.1155/2022/6068185

Cited by 12 publications

(8 citation statements)

References 52 publications

(54 reference statements)

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“…Since all tested mushrooms were cultivated in similar conditions, this variability may be mainly caused by genetic differences and subsequent changes in metabolic pathways. According to Lin et al’s [ 45 ] phylogenetic analysis of Pleurotus species, P. ostreatus and P. flabellatus are the most distinct among the tested species, which is in agreement with the results of our metabolomic and bioactivity investigations, wherein usually these two species are the most distinct.…”

Section: Resultssupporting

confidence: 90%

Antioxidant and Anti-Inflammatory Activity of Five Medicinal Mushrooms of the Genus Pleurotus

et al. 2022

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Within the group of higher fungi, edible medicinal mushrooms have a long history of being used as food and in folk medicine. These species contain biologically active substances with many potential beneficial effects on human health. The Pleurotus genus is representative of medicinal mushrooms because Pleurotus ostreatus is one of the most commonly cultivated culinary mushrooms. In our study, we focused on lesser-known species in the genus Pleurotus and measured their antioxidant and anti-inflammatory activity. We prepared extracts of the mushrooms and analyzed them using HPLC−HRMS, GC−MS, and 1H-NMR. Significant differences in biological activities were found among the Pleurotus spp. extracts. A MeOH extract of P. flabellatus was the most active as a radical scavenger with the highest ORAC, while a chloroform extract had significant anti-inflammatory COX-2 activity. The 80% MeOH extract of P. flabellatus contained the highest amounts of ergosterol, ergothioneine, and mannitol. The 80% MeOH extract of P. ostreatus Florida was the most active in the NF-κB inhibition assay and had the highest content of β-glucans (43.3% by dry weight). Given the antioxidant and anti-inflammatory properties of P. flabellatus, the potential therapeutic usefulness of this species is worth evaluating through in-depth investigations and confirmation by clinical trials.

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Section: Resultssupporting

confidence: 90%

Antioxidant and Anti-Inflammatory Activity of Five Medicinal Mushrooms of the Genus Pleurotus

et al. 2022

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“…However, attempts to transmit OMSV to P. eryngii , P. salmoneostramineus , and P. giganteus were unsuccessful, likely due to a robust transmission barrier between the test strains. In addition, phylogenetic analyses suggested a closer evolutionary relationship between P. pulmonarius and P. floridanus but indicated a distant evolutionary relationship with P. eryngii , P. giganteus , and P. salmoneostramineus [ 34 ]. This implied the existence of a more formidable interspecies transmission barrier between these two non-transmissible species.…”

Section: Discussionmentioning

confidence: 99%

Transmission of Oyster Mushroom Spherical Virus to Progeny via Basidiospores and Horizontally to a New Host Pleurotus floridanus

Wang,

Wen,

Yang

et al. 2024

IJMS

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Mycoviruses are usually transmitted horizontally via hyphal anastomosis and vertically through sporulation in natural settings. Oyster mushroom spherical virus (OMSV) is a mycovirus that infects Pleurotus ostreatus, with horizontal transmission via hyphal anastomosis. However, whether OMSV can be vertically transmitted is unclear. This study aimed to investigate the transmission characteristics of OMSV to progeny via basidiospores and horizontally to a new host. A total of 37 single-basidiospore offspring were obtained from OMSV-infected P. ostreatus and Pleurotus pulmonarius for Western blot detection of OMSV. The OMSV-carrying rate among monokaryotic isolates was 19% in P. ostreatus and 44% in P. pulmonarius. Then, OMSV-free and OMSV-infected monokaryotic isolates were selected for hybridization with harvested dikaryotic progeny strains. Western blot analyses of the offspring revealed that the OMSV transmission efficiency was 50% in P. ostreatus and 75% in P. pulmonarius, indicating vertical transmission via sexual basidiospores. Furthermore, we observed the horizontal transfer of OMSV from P. pulmonarius to Pleurotus floridanus. OMSV infection in P. floridanus resulted in significant inhibition of mycelial growth and yield loss. This study was novel in reporting the vertical transmission of OMSV through basidiospores, and its infection and pathogenicity in a new host P. floridanus.

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“…Furthermore, the possibility that a strong transmissibility barrier between the two test strains exists cannot be discounted. According to the phylogenetic studies of species in the Pleurotus genus, P. ostreatus clusters with P. pulmonarius, and they have a closer relationship than with other species ( P. eryngii , P. citrinopileatus , P. nebrodensis , or P. salmoneostramineus ) [ 39 ], suggesting a stronger interspecies transmission barrier between the two non-transmissible species.…”

Section: Discussionmentioning

confidence: 99%

Oyster Mushroom Spherical Virus Crosses the Species Barrier and Is Pathogenic to a New Host Pleurotus pulmonarius

Zhang,

Hu,

Zhao

et al. 2023

IJMS

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Oyster mushroom spherical virus (OMSV) is a mycovirus with a positive-sense single-stranded RNA genome that infects the edible mushroom Pleurotus ostreatus. OMSV is horizontally transferred from an infected strain to a cured strain via mycelia. The infection results in significant inhibition of mycelial growth, malformation of fruiting bodies, and yield loss in oyster mushrooms. This study successfully transferred OMSV from P. ostreatus to Pleurotus pulmonarius. However, transmission was not successful in other Pleurotus species including P. citrinopileatus, P. eryngii, P. nebrodensis, and P. salmoneostramineus. The successful OMSV infection in P. pulmonarius was further verified with Western blot analysis using a newly prepared polyclonal antiserum against the OMSV coat protein. Furthermore, OMSV infection reduced the mycelial growth rate of P. pulmonarius. The OMSV-infected strain demonstrated abnormal performance including twisted mushrooms or irregular edge of the cap as well as reduced yield of fruiting bodies in P. pulmonarius, compared to the OMSV-free strain. This study is the first report on the infection and pathogenicity of OMSV to the new host P. pulmonarius. The data from this study therefore suggest that OMSV is a potential threat to P. pulmonarius.

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Genetic and Chemical Diversity of Edible Mushroom Pleurotus Species

Cited by 12 publications

References 52 publications

Antioxidant and Anti-Inflammatory Activity of Five Medicinal Mushrooms of the Genus Pleurotus

Antioxidant and Anti-Inflammatory Activity of Five Medicinal Mushrooms of the Genus Pleurotus

Transmission of Oyster Mushroom Spherical Virus to Progeny via Basidiospores and Horizontally to a New Host Pleurotus floridanus

Oyster Mushroom Spherical Virus Crosses the Species Barrier and Is Pathogenic to a New Host Pleurotus pulmonarius

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