Molecular survey of Anaplasma and Ehrlichia species in livestock ticks from Kassena-Nankana, Ghana; with a first report of Anaplasma capra and Ehrlichia minasensis

Addo, Seth Offei; Baako, Bernice Olivia Ama; Bentil, Ronald Essah; Addae, Charlotte Adwoa; Behene, Eric; Asoala, Victor; Sallam, Mohamed F.; Mate, Suzanne; Dunford, James C.; Larbi, John Asiedu; Baidoo, P. K.; Wilson, Michael D.; Diclaro, Joseph W.; Dadzie, Samuel

doi:10.1007/s00203-023-03430-1

Cited by 9 publications

(7 citation statements)

References 92 publications

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“…In cattle, infections result in fever, reduced milk production, and infertility [ 35 ]. The finding of this study can be compared to a previous study in Ghana that found A. phagocytophilum infection in cattle [ 36 ]. The first human case was reported in the United States [ 37 ] and has since spread to numerous countries including China [ 38 ] and Canada [ 39 ].…”

Section: Discussionmentioning

confidence: 60%

Molecular Detection of Tick‐Borne Pathogens in Kumasi: With a First Report of Zoonotic Pathogens in Abattoir Workers

Addo,

Amoah,

Unicorn

et al. 2024

BioMed Research International

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Tick‐borne pathogens continue to infect humans and animals worldwide. By adapting to the movement of livestock, ticks facilitate the spread of these infectious pathogens. Humans in close contact with animals that could be amplifying hosts are especially at risk of being infected with tick‐borne pathogens. This study involved the collection of dry blood spots (DBSs) to determine tick‐borne pathogens occurring in slaughtered livestock and abattoir workers in Kumasi. This study employed the use of conventional PCR, RT‐PCR, and Sanger sequencing to detect and identify the tick‐borne pathogens. The resulting data was analysed using Stata version 13. A total of 175 DBSs were collected from goats (76), cattle (54), and sheep (45) in the Kumasi abattoir (130, 74.29%) and Akwatia Line slaughter slab (45, 25.71%). The pathogens identified were mostly bacterial including Anaplasma capra (9.71%), Anaplasma phagocytophilum (1.14%), and Rickettsia aeschlimannii (0.57.%). The only parasite identified was Theileria ovis (9.14%). A significant association was seen between A. capra (p < 0.001) infection and female sheep sampled from the Akwatia Line slaughter slab. Again, there was a significant association between T. ovis (p < 0.001) infections and female sheep from the Kumasi abattoir. From the human DBS (63) screened, the pathogens identified were all bacterial including Coxiella burnetii (1.89%), Rickettsia africae (1.89%), and R. aeschlimannii (1.89%). This study reports the first detection of R. aeschlimannii in livestock as well as the occurrence of the above‐mentioned pathogens in humans in Ghana. Animals can serve as amplifying hosts for infectious pathogens; hence, there is an increased risk of infections among the abattoir workers. Continuous surveillance effort is essential, and abattoir workers need to protect themselves from tick bites and infectious tick‐borne pathogens.

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Section: Discussionmentioning

confidence: 60%

Molecular Detection of Tick‐Borne Pathogens in Kumasi: With a First Report of Zoonotic Pathogens in Abattoir Workers

Addo,

Amoah,

Unicorn

et al. 2024

BioMed Research International

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“…Previous studies have reported this species in ruminants (sheep, goats, cattle, and buffalo), domestic dogs and wild animals (e.g., musk deer, Himalayan goral, and Korean water deer). This species was observed in sheep, cattle, and buffalo raised in Sivas, Turkey (Altay et al 2022;Sahin et al 2022;Addo et al 2023). Recent studies in Turkey have shown that goats can act as a natural reservoir for Anaplasma phagocytophilum-like 1 and A. ovis (Altay et al 2014;Zhou et al 2017;Erol et al 2022).…”

Section: Introductionmentioning

confidence: 95%

First Molecular Anaplasma capra Detection in Turkish Goats

Oğuz

Değer

Al-Olayan³

et al. 2023

Preprint

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Anaplasma capra infection is usually asymptomatic, but it is known to cause zoonotic tick-borne diseases. A. capra's morphological characteristics and the types of cells infected (such as erythrocytes, monocytes, and neutrophil granulocytes) are unknown. Infection with A. capra was mostly found in ixodid ticks. There have been no studies on A. capra infection in goats, one of Turkey's most common farm animals. Infection with A. capra was mostly found in ixodid ticks. There have been no studies on A. capra infection in goats, one of Turkey's most common farm animals. They are required to determine their distribution, genetic diversity, vector species, and host specificity. This study aimed to investigate the A. capra pathogen in goats in Turkey's Van province. A total of 200 goat blood samples were examined. Goat samples were subjected to partial amplification of the gltA gene fragment using a nested polymerase chain reaction. A. capra DNA was detected in 0.5% of goat blood samples. Phylogenetic analysis of a partial gltA gene fragment showed that the eastern Turkey isolate, closely grouped with A. capra isolates reported from wild and domestic ruminants in France, Turkey, and Kyrgyzstan, formed a distinct clade. This is the first report of A. capra in goats in Turkey.

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“…Haemaphysalis longicornis is the tick species on which the most studies have been conducted and for which A. capra has been detected the most (Guo et al 2018 ; Lu et al 2023 ; Qin et al 2018 ; Seo et al 2020 ; Sun et al 2015 ; Teng et al 2023 ; Yan et al 2021 ). In much more limited work, A. capra was detected in H. ginghainensis (Han et al 2019 ; Yang et al 2016 ), Ixodes persulcatus (Li et al 2015 ), I. kashmiricus (Numan et al 2023 ), Rhipicephalus sanguineus (unpublished data, GenBank; OK091153), R. microplus ( Addo et al 2023 ; Guo et al 2018 , 2019 ) Dermacontor nuttali, D. everestianus , and D. abaensis (Han et al 2019 ).…”

Section: The Hosts Of Anaplasma Capramentioning

confidence: 99%

“…The positivity rate in ticks is quite variable. It was detected in I. persulcatus at 3.0% (Li et al 2015 ), in H. longicornis at 0.43–63.27% (Guo et al 2018 ; Lu et al 2023 ; Seo et al 2020 ; Sun et al 2015 ; Teng et al 2023 ; Yan et al 2021 ;), in H. qinghaiensis at 4.5–5.8% (Han et al 2019 ; Yang et al 2016 ), in R. microplu s at 0.81–40.4% positive rates (Addo et al 2023 ; Guo et al 2018 , 2019 ). Almost all of the studies on ticks were conducted in China, where A. capra was first detected and where most studies were conducted on vertebrates.…”

Section: The Hosts Of Anaplasma Capramentioning

confidence: 99%

“…Although the studies on A. capra were mostly conducted in China, in a short time its presence has been revealed in 18 different countries including China (Liu et al 2012 ), South Korea (Seo et al 2018 ), Türkiye (Altay et al 2022a ), Kyrgyztan (Altay et al 2022b , c ), Malaysia (Koh et al 2018 ), Japan (Kawahara et al 2006 ), Iraq (unpublished data GenBank; ON872236), Iran (Staji et al 2021 ), India (Kumar et al 2023 ), Pakistan (Isaq et al 2022), France (Jouglin et al 2019 ), Sweeden (Grandi et al 2018 ), Portugal (unpublished data, GenBank; OK091153), Spain (Remesar et al 2022 ), Greece (Saratsis et al 2022 ), Angola (Barradas et al 2021 ), Morocco (Elhachimi et al 2021 ), and Ghana (Addo et al 2023 ) by molecular techniques (Fig. 1 ).…”

Section: The Molecular Prevalence Of Anaplasma Capramentioning

confidence: 99%

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Anaplasma capra: a new emerging tick-borne zoonotic pathogen

Altay,

Erol,

Sahin

2024

Vet Res Commun

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The genus Anaplasma includes A. marginale, A. centrale, A. bovis, A. ovis, A. platys, and A. phagocytophilum transmitted by ticks, some of which are zoonotic and cause anaplasmosis in humans and animals. In 2012, a new species was discovered in goats in China. In 2015, the same agent was detected in humans in China, and it was provisionally named Anaplasma capra, referring to 2012. The studies conducted to date have revealed the existence of A. capra in humans, domestic animals, wild animals, and ticks from three different continents (Asia, Europe, and Africa). Phylogenetic analyses based on gltA and groEL sequences show that A. capra clearly includes two different genotypes (A. capra genotype-1 and A. capra genotype-2). Although A. capra human isolates are in the genotype-2 group, goat, sheep, and cattle isolates are in both groups, making it difficult to establish a host genotype-relationship. According to current data, it can be thought that human isolates are genotype-2 and while only genotype-1 is found in Europe, both genotypes are found in Asia. Anaplasma capra causes clinical disease in humans, but the situation is not yet sufficient to understand the zoonotic importance and pathogenicity in animals. In the present review, the history, hosts (vertebrates and ticks), molecular prevalence, pathogenic properties, and genetic diversity of A. capra were evaluated from a broad perspective.

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Molecular survey of Anaplasma and Ehrlichia species in livestock ticks from Kassena-Nankana, Ghana; with a first report of Anaplasma capra and Ehrlichia minasensis

Cited by 9 publications

References 92 publications

Molecular Detection of Tick‐Borne Pathogens in Kumasi: With a First Report of Zoonotic Pathogens in Abattoir Workers

Molecular Detection of Tick‐Borne Pathogens in Kumasi: With a First Report of Zoonotic Pathogens in Abattoir Workers

First Molecular Anaplasma capra Detection in Turkish Goats

Anaplasma capra: a new emerging tick-borne zoonotic pathogen

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