Molecular surgery of DNA based on electrostatic micromanipulation

Yamamoto, Takatoki; Kurosawa, Osamu; Kabata, Hiroyuki; Shimamoto, Nobuo; Washizu, Masao

doi:10.1109/28.855954

Cited by 93 publications

(39 citation statements)

References 6 publications

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“…DEP is the movement of particles in a nonuniform electric field, and it can occur in AC or DC electric fields. DEP has been successfully applied for the manipulation of a wide range of particles: biomolecules [34][35][36][37][38], virus [39][40][41], bacteria [42][43][44][45][46][47][48][49], spores [50,51], mammalian cells [52][53][54] and parasites [55][56][57]. DEP offers the possibility of sample concentration and separation in a single step.…”

Section: Atps and Dielectrophoresismentioning

confidence: 99%

Extraction and Purification of Bioproducts and Nanoparticles using Aqueous Two‐Phase Systems Strategies

et al. 2008

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Aqueous Two-Phase Systems (ATPS) is a primary recovery technique that has shown great potential for the efficient extraction and purification of high value biological compounds. The main advantages of this technique include scaling up feasibility, process integration capability and biocompatibility. In this review, the efficient use of ATPS for the extraction of proteins, genetic material, low molecular weight compounds, bioparticles, nanoparticles and cells is highlighted. The important role of ATPS in process integration, i.e., extractive conversion, extractive fermentation, cell disruption integrated with product recovery, and extractive purification, is discussed. A novel approach to protein molecular characterization combining ATPS and 2-dimension electrophoresis (2-DE) is introduced as a first step in the process development. Novel approaches for downstream processing using ATPS and dielectrophoresis are presented. Finally, trends concerning the application of ATPS strategies to address the future challenges of bioseparation are discussed.

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Section: Atps and Dielectrophoresismentioning

confidence: 99%

Extraction and Purification of Bioproducts and Nanoparticles using Aqueous Two‐Phase Systems Strategies

et al. 2008

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“…5,15,16 Recently the dielectrophoretic manipulation of DNA molecules has led to applications such as the concentration of DNA molecules, [17][18][19] DNA-protein interaction studies, 20,21 and molecular surgery of DNA. 22 However, a detailed understanding of the behavior of surface-immobilized DNA molecules when exposed to high frequency ac electric fields, and in particular the orientation and elongation of DNA as a result of dielectrophoretic force and torque, has not yet been established. This is partly owing to the complex local conditions that exist during application of the electric field.…”

Section: Introductionmentioning

confidence: 99%

Influence of alternating current electrokinetic forces and torque on the elongation of immobilized DNA

Germishuizen

Tosch

Middelberg

et al. 2004

Journal of Applied Physics

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Articles you may be interested inThe authors investigate the elongation and orientation of different-sized deoxyribose nucleic acid (DNA) molecules, tethered onto gold electrodes via a terminal thiol, under the influence of high frequency ac electric fields. The DNA molecules are elongated from a random coil into an extended conformation and orientated along the electric field lines as a result of the forces acting on the molecules during the application of the ac electric fields. Elongation was observed in the frequency range 100 kHz-1 MHz, with field strengths of 0.06-1.0 MV/ m. Maximum elongation for all DNA fragments tested, irrespective of size, was found for frequencies between 200 and 300 kHz. The torque acting on the induced dipole in the DNA molecules, complemented by a directional bias force, opposite in direction to the dielectrophoretic force, provides the main contribution to the elongation process. The length of elongation is limited to either half the distance between opposing electrodes or to the contour length of the DNA, whichever is shorter. Further, the authors show that the normalized length of the elongated DNA molecules is independent of the contour length of the DNA.

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“…In contrast to many dielectrophoresis experiments in which the DNA is free in solution [4][5][6][9][10][11][12][13], here the DNA is tethered to a surface by strong thiol-gold bonds. The average maximum length of the elongated DNA depends on the frequency and the magnitude of the ac field [4].…”

Section: Dielectrophoretic Stretching Of Surface-bound Dnamentioning

confidence: 99%

“…This alignment is due to the torque acting on the dominant induced dipole moment, which occurs axially along the DNA [10][11][12], and forms a band-like structure around the electrodes (figure 1). In terms of the dipole moment, p, and the electric field, E, the torque experienced by the DNA is given by T = p × E. The dipole moment depends on the permittivity of the liquid and the DNA, respectively, as well as on the electric field, E, and the length of the DNA [19].…”

Section: Dielectrophoretic Stretching Of Surface-bound Dnamentioning

confidence: 99%

“…However, a detailed and quantitative understanding of the dielectrophoresis of DNA is required to tap the full potential of the technique. Dielectrophoretic manipulation of DNA has been widely studied in the past and a large number of applications have emerged, including size determination of DNA, site-specific immobilization of DNA [6,9], molecular surgery [6,10], DNA sequencing [11][12][13] and dielectric characterization of DNA fragments [14]. The use of microelectrodes allows the generation of the strong electric fields necessary for dielectrophoresis (of the order of 1 MV m −1 ) using relatively small potentials.…”

Section: Introductionmentioning

confidence: 99%

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Selective dielectrophoretic manipulation of surface-immobilized DNA molecules

et al. 2003

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The fabrication of nanoscale molecular devices is becoming increasingly important and research into their fabrication has intensified over the last few years. In particular, the attachment of molecular objects onto various surfaces has attracted considerable attention. Here, we report a multistep surface immobilization procedure, which allows the specific and controlled attachment of very long DNA molecules onto gold electrodes. Further, we report the effect of dielectrophoresis on these surface-bound DNA molecules with respect to amplitude and frequency, and we show that selected surface-immobilized DNA molecules can be manipulated by dielectrophoresis. Finally, we investigated the use of dielectrophoresis in conjunction with the multistep surface immobilization of fluorescently labelled, surface-bound λ-DNA in a basic data-storage device.

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Molecular surgery of DNA based on electrostatic micromanipulation

Cited by 93 publications

References 6 publications

Extraction and Purification of Bioproducts and Nanoparticles using Aqueous Two‐Phase Systems Strategies

Extraction and Purification of Bioproducts and Nanoparticles using Aqueous Two‐Phase Systems Strategies

Influence of alternating current electrokinetic forces and torque on the elongation of immobilized DNA

Selective dielectrophoretic manipulation of surface-immobilized DNA molecules

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