Molecular Subtyping of Borrelia burgdorferi in Erythema Migrans and Acrodermatitis Chronica Atrophicans

Wienecke, Ralf; Zöchling, Natalie; Neubert, U.; Schlüpen, Eva-Maria; Meurer, Michael; Volkenandt, Matthias

doi:10.1111/1523-1747.ep12388947

Cited by 66 publications

(35 citation statements)

References 20 publications

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“…In all specimens of patients with ACA, only B. afzelii was detected. These data indicate that ACA might be closely associated with this genospecies (12,125,187).…”

Section: Patients With Dermatological Manifestationsmentioning

confidence: 57%

“…Subtyping of B. burgdorferi sensu lato strains in clinical samples from infected patients has been reported by several groups (9,12,125,187). Isolates from skin lesions of patients with ACA were always B. afzelii, whereas in all patients with LA, B. burgdorferi sensu stricto was found.…”

Section: Subtyping Of B Burgdorferi Sensu Lato In Infected Patientsmentioning

confidence: 82%

“…Isolates from skin lesions of patients with ACA were always B. afzelii, whereas in all patients with LA, B. burgdorferi sensu stricto was found. Patients with EM can harbor all three genospecies of B. burgdorferi sensu lato, as analyzed in a German study: 28 of 35 were B. afzelii, 6 of 35 were B. garinii, and 1 of 35 was B. burgdorferi sensu stricto (187). CSF from patients with neuroborreliosis has been found to harbor only B. afzelii (9), either B. afzelii or B. burgdorferi sensu stricto (12), or one of all three genospecies (32).…”

Section: Subtyping Of B Burgdorferi Sensu Lato In Infected Patientsmentioning

confidence: 99%

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Methodological Aspects

1985

Journal of Internal Medicine

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“…In all specimens of patients with ACA, only B. afzelii was detected. These data indicate that ACA might be closely associated with this genospecies (12,125,187).…”

Section: Patients With Dermatological Manifestationsmentioning

confidence: 57%

Section: Subtyping Of B Burgdorferi Sensu Lato In Infected Patientsmentioning

confidence: 82%

Section: Subtyping Of B Burgdorferi Sensu Lato In Infected Patientsmentioning

confidence: 99%

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Methodological Aspects

1985

Journal of Internal Medicine

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“…In these studies, an exclusive association between the late cutaneous manifestation acrodermatitis chronica atrophicans and B. afzelii infection (1,2,16,20) was demonstrated. Reports of CSF isolates from patients suffering from neuroborreliosis were rather heterogeneous, but B. garinii strains were much more often involved than B. burgdorferi sensu stricto or B. afzelii (4,10,21).…”

mentioning

confidence: 93%

Rapid Typing of Borrelia burgdorferi Sensu Lato Species in Specimens from Patients with Different Manifestations of Lyme Borreliosis

et al. 2001

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To further investigate the pathogenic potential of different Borrelia burgdorferi genospecies, specimens from 27 patients with different manifestations of Lyme borreliosis were analyzed by PCR and reverse line blotting (RLB). In samples from Lyme arthritis patients, B. burgdorferi sensu stricto was predominantly identified, while in patients with neuroborreliosis or acrodermatitis, Borrelia garinii and Borrelia afzelii, respectively, were exclusively detected. The results demonstrate that PCR-RLB is a valuable tool for epidemiological and pathogenetic studies of Lyme borreliosis.

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“…The fragment of the p66 gene (used here for the first time in LightCycler PCR, to our knowledge) has a wide heterogeneity in the three species and can be amplified in two steps with nested primers (14,24). Molecular subtyping was performed with the same sequence of the p66 gene by analysis of cRNA single-strand conformation polymorphisms (26). Interestingly, a published PCR protocol yielded greater sensitivities for most clinical samples using the p66 nested primer set compared to another nested primer set targeting the plasmid gene ospA (12).…”

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confidence: 99%

Sensitive Detection of Borrelia burgdorferi Sensu Lato DNA and Differentiation of Borrelia Species by LightCycler PCR

et al. 2001

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In order to differentiate species within the Borrelia burgdorferi sensu lato complex, LightCyler PCR and melting-curve analysis of the amplicons of two genes with intraspecies variability, the p66 gene and the recA gene, were performed. It was demonstrated that nested LightCycler PCR amplification of p66 is more sensitive in the detection of borrelia DNA than amplification of the recA gene. B. burgdorferi sensu stricto could be differentiated from Borrelia garinii and Borrelia afzelii by melting-curve analysis of the p66 gene amplicon. B. garinii could be differentiated from B. afzelii and B. burgdorferi sensu stricto by melting-curve analysis of the recA gene amplicon. Therefore, the PCRs complement each other in subtyping different Borrelia species, and combined LightCycler PCR and melting-curve analysis of both target genes is a rapid method to distinguish the three species of B. burgdorferi sensu lato.Lyme disease is the most prevalent tick-borne disease of the Northern Hemisphere (3). Its etiologic agent, Borrelia burgdorferi sensu lato, has been divided into different species. B. burgdorferi sensu stricto, Borrelia afzelii, and Borrelia garinii are the common human pathogenic species (4, 21). The infection leads to a variety of clinical symptoms involving the skin, nervous system, heart, and joints (19).Erythema migrans (EM) and acrodermatitis chronica atrophicans (ACA) represent common cutaneous manifestations of an infection by B. burgdorferi sensu lato (1, 13, 21), whereas the role of this spirochete in the pathogenesis of morphea and lichen sclerosus et atrophicus is controversial (13,15,23,25).Since PCR has proved to be a sensitive and fast method for the diagnosis of microrganisms which are difficult to culture, the technique has been applied to the detection of B. burgdorferi sensu lato DNA in infected ticks (6) as well as in human specimens, such as cerebrospinal fluid (4) or synovial fluid (5, 20), urine (2, 16), and skin (9, 24). Established PCR protocols amplify different segments of borrelial chromosomal genes, such as the flagellin gene (10, 15), the one-copy 16S rRNA gene (7), the 23S rRNA gene (17), the p66 gene segment encoding a 66-kDa protein (14), the recA gene (8), and the plasmid-encoded ospA gene (9,20).Common PCR with a conventional thermocycler and subsequent separation of the amplicon by agarose gel electrophoresis allows the detection of B. burgdorferi sensu lato DNA. Subtyping of Borrelia species DNA is not possible, since the intraspecies sequence polymorphisms of PCR amplicons are only a few base pairs long.Several postamplification methods were employed to identify Borrelia species commonly associated with Lyme borreliosis, e.g., oligonucleotide typing with PCR fragments (5), randomly amplified polymorphic DNA fingerprinting analysis (22), pulsed-field gel electrophoresis (4), single-strand conformation polymorphism (18), and subtype-specific PCR targeting the 16S rRNA gene (6). These techniques are usually time-consuming, and some of them require high technical standards and ...

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Molecular Subtyping of Borrelia burgdorferi in Erythema Migrans and Acrodermatitis Chronica Atrophicans

Cited by 66 publications

References 20 publications

Methodological Aspects

Methodological Aspects

Rapid Typing of Borrelia burgdorferi Sensu Lato Species in Specimens from Patients with Different Manifestations of Lyme Borreliosis

Sensitive Detection of Borrelia burgdorferi Sensu Lato DNA and Differentiation of Borrelia Species by LightCycler PCR

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