Molecular study to detect the Eimeria species in sheep in Al-Diwaniyah province, Iraq

Abstract

Sheep eimriosis is one of the most important and common disease which infects sheep in all ages but it is more effective in lambs. The diarrhea with or without blood is the main signs of infection. Eimeria protozoan required single host to complete its life cycle which pass in different stages including schizogony, gametogony and sporogony. The study was designed for detection of sheep Eimeria species through the molecular method. This study was conducted in Al-Diwanyah province during the winter months of 2019. In which 200 sheep fecal samples were collected and examined traditionally to investigate the Eimeria oocytsts. Ninety-seven samples of highly intensity infection with Eimeria oocysts were selected to subject for DNA extraction process. The extracted DNAs were tested through amplification of internal transcribed spacer 1 (ITS-1) gene by conventional PCR, and then phylogenetic analysis was made to diagnose the sheep Eimeria species. All samples that examined microscopically were showed positive results of infections with Eimeria protozoan. Out of 97 molecularly examined samples, forty-five (46.39%) were given positive result in conventional PCR technique, where Eimeria spp. detected through succeeded amplification of internal transcribed spacer 1 (ITS-1) gene. Then phylogenetic analysis referred to that there are five species of Eimeria confirmed in sheep in Al-Diwanyah province including 6 (33.33%) samples diagnosed as E. ahsata, 4 (22.22%) samples E. weybridgensis, 3 (16.66%) samples E. ovinoidalis, 3 (16.66%) samples E. bovis and 2 (11.11%) samples E. auburnensis. So, the Eimeria protozoan appears as an endemic parasite and can infect sheep with different species in study area. The sheep can infect with both specific and nonspecific species.

Prevalence of border disease virus in sheep and goats in Mosul, Iraq

“…The study also revealed that the molecular technique is an accurate and sensitive method for detection and identification of the causative pathogen. This finding is consistent with what mention in literature of (46,47).…”

Dry Season Eimeria Infection in Dairy Cattle and Sheep in and Around Adama and Bishoftu Towns, Oromia, Ethiopia

“…26 Adult animals are usually asymptomatic, though they can serve as a reservoir for younger animals. 42 Cattle of all ages can become infected, but symptomatic Eimeriosis is most common in young animals. 51 The disease is spread through the consumption of contaminated food and water and symptoms include diarrhea with mucus and blood, lack of appetite, weight loss, anemia, fatigue, wool breaking and eventually death of the animal.…”

Some physiological and biochemical criteria in the local buffalo infected with stomach and intestinal worms in the city of Samarra

“…It was transferred to 15 mL test tubes and placed in the centrifuge for 3 minutes. After that the floating was remove and taking part of precipitate and placed on a glass slide with cover slid and examined under a microscope (10).…”

“…Using the digital blood count device, the total number of red blood cells, hemoglobin concentration, packed cell volume, total number of platelets, and total and differential white blood cells count were measured (11,12).…”