Molecular study on the role of vacuolar transporters in glycyrrhetinic acid production in engineered Saccharomyces cerevisiae

Alkhadrawi, Adham M; Xue, Haijie; Ahmad, Nadeem; Akram, Muhammad; Wang, Ying; Li, Chun

doi:10.1016/j.bbamem.2022.183890

Cited by 6 publications

(3 citation statements)

References 53 publications

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“…Notably, the synthesis of terpenoids certainly passes through various organelles, such as ER, Golgi LD, and so on. To promote extracellular secretion, further enhancement of intracellular transportation can theoretically improve terpenoid synthesis to a considerable extent [ 123 ]. However, research on accelerating intracellular transportation is still in its infancy and more organelle transporters should be mined (Fig.…”

Section: Discussionmentioning

confidence: 99%

Compartmentalization and transporter engineering strategies for terpenoid synthesis

Jin

Xia²,

Liu

et al. 2022

Microb Cell Fact

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Microbial cell factories for terpenoid synthesis form a less expensive and more environment-friendly approach than chemical synthesis and extraction, and are thus being regarded as mainstream research recently. Organelle compartmentalization for terpenoid synthesis has received much attention from researchers owing to the diverse physiochemical characteristics of organelles. In this review, we first systematically summarized various compartmentalization strategies utilized in terpenoid production, mainly plant terpenoids, which can provide catalytic reactions with sufficient intermediates and a suitable environment, while bypassing competing metabolic pathways. In addition, because of the limited storage capacity of cells, strategies used for the expansion of specific organelle membranes were discussed. Next, transporter engineering strategies to overcome the cytotoxic effects of terpenoid accumulation were analyzed. Finally, we discussed the future perspectives of compartmentalization and transporter engineering strategies, with the hope of providing theoretical guidance for designing and constructing cell factories for the purpose of terpenoid production.

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Section: Discussionmentioning

confidence: 99%

Compartmentalization and transporter engineering strategies for terpenoid synthesis

Jin

Xia²,

Liu

et al. 2022

Microb Cell Fact

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“…The addition of the two transporter protein genes AcrB and TolC can increase the production of amorphadiene and kaurene . Yeast ATP-binding cassette (ABC) vacuolar transporters can increase the production of glycyrrhetinic acid and BPT1, which can in turn promote the production of glucoamylase . PDR11 is primarily responsible for the export of rubusoside, and PDR11 overexpression can increase the concentration of rubusoside by 129.8% .…”

Section: Introductionmentioning

confidence: 99%

“…28 Yeast ATP-binding cassette (ABC) vacuolar transporters can increase the production of glycyrrhetinic acid and BPT1, which can in turn promote the production of glucoamylase. 29 PDR11 is primarily responsible for the export of rubusoside, and PDR11 overexpression can increase the concentration of rubusoside by 129.8%. 30 Hence, identifying transporters that could mediate the transport of CBGA from the cytoplasm to the vacuole could greatly enhance CBDA production.…”

Section: Introductionmentioning

confidence: 99%

Boosting the Cannabidiol Production in Engineered Saccharomyces cerevisiae by Harnessing the Vacuolar Transporter BPT1

Qiu

Hou

et al. 2022

J. Agric. Food Chem.

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Cannabidiol (CBD), the main nonpsychoactive cannabinoid in Cannabis sativa, has diverse applications in the pharmacological, food, and cosmetic industries. The long plantation period and the complex chemical structure of cannabidiol pose a great challenge on CBD supply. Here, we achieved de novo biosynthesis of cannabidiol in Saccharomyces cerevisiae. The CBD production was further enhanced by 2.53-fold through pushing the supply of precursors and fusion protein construction. Bile pigment transporter 1 (BPT1) was the most effective transporter for transferring cannabigerolic acid (CBGA) from the cytoplasm to the vacuole, which removed the physical barrier separating CBGA and its catalytic enzyme. The lowest binding energy of the CBGA–BPT1 complex confirmed a strong interaction between BPT1 and CBGA. A CBD yield of 6.92 mg/L was achieved, which was 100-fold higher than the yield generated by the starting strain. This study provides insights into high-level CBD-producing strain construction and lays the foundation for CBD supply.

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Low temperature promotes the production and efflux of terpenoids in yeast

Qin,

Ma,

Lin

et al. 2024

Bioresource Technology

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Molecular study on the role of vacuolar transporters in glycyrrhetinic acid production in engineered Saccharomyces cerevisiae

Cited by 6 publications

References 53 publications

Compartmentalization and transporter engineering strategies for terpenoid synthesis

Compartmentalization and transporter engineering strategies for terpenoid synthesis

Boosting the Cannabidiol Production in Engineered Saccharomyces cerevisiae by Harnessing the Vacuolar Transporter BPT1

Low temperature promotes the production and efflux of terpenoids in yeast

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