Molecular study of persistence of Nocardia asteroides and Nocardia otitidiscaviarum strains in patients with long-term nocardiosis

Abstract

Three Nocardia asteroides and five Nocardia otitidiscaviarum isolates recovered from three patients with longterm nocardiosis were compared by random amplification of polymorphic DNA fingerprinting, antibiotic susceptibility testing, and enzymatic characterization. Results obtained with primer DKU49 (P. Palittapongarnpim, S. Chomyc, A. Fanning, and D. Kunimoto, J. Infect. Dis. 167:975-978, 1993) provide evidence that patient A was infected by two N. asteroides strains during a single episode of nocardiosis and that patients B and C remained infected by the same strain, respectively. Resistance to minocycline that was present in the first isolate recovered from patient B reverted to intermediate resistance in the second isolate and reverted to susceptibility in the third isolate. Resistance to penicillin G and ␤-lactams was acquired by the second isolate obtained from patient C.

Nocardia Thyroiditis: Unusual Location of Infection

“…For this reason surgical drainage was necessary. As has already been described, we noted that the mechanism of penicillin resistance of our strain implicated the production of beta-lactamase (16). The beta-lactamase was detected as published previously (19) by the addition of clavulanic acid to commercial amoxicillin disks and observation of a zone of inhibition greater than that achieved with amoxicillin alone.…”

Antibacterial activity of some actinomycetes from Tamil Nadu, India

“…The active isolate VAS 10 was identified as Actinobacterium Loyola PBT VAS 10 using 16S rRNA studies. In contrast to the traditional methods of actinomycetes identification, which are slow, difficult and very expensive, the molecular approach for identification is often used for their speed and efficiency [27] . Highest similarity (98%) was obtained with the 16S rRNA gene of Streptomyces roseoflavus which produced the aminoglycoside antibiotic flavomycin [28] .…”

Nocardia nova as the Causative Agent in Spondylodiscitis and Psoas Abscess

“…Comparison of the relapse isolates with the agent from the primary pulmonary infection was performed by randomly amplified polymorphic DNA analysis, using the DKU49 (5Ј-CCG CCGACCGAG-3Ј) primer as previously described (13). This procedure established that the isolates from the second and the third samples were of the same genotype as the first isolate.…”