Antibacterial activity of some actinomycetes from Tamil Nadu, India

Kumar, Pachaiyappan Saravana; Raj, J.; Duraipandiyan, Veeramuthu; Ignacimuthu, Savarimuthu

doi:10.1016/s2221-1691(13)60003-9

Cited by 46 publications

(24 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this study, brown was the predominant color, in contrast to those observed by Ramos et al (2015) and Silva et al (2015), who in characterizing actinobacteria strains coming from the semiarid region by cultures noted the predominance of gray, cream and white. This color variation is widely reported in the literature in actinobacteria strains originating from different regions, such as the Himalayas (Duraipandiyan et al, 2010), India (Kumar et al, 2010;Kumar et al, 2012;Das et al, 2014;Amsaveni et al, 2015), China (Yu et al, 2015), Egypt (Mabrouk e Saleh, 2014) and Iraq (Jaralla et al, 2014).…”

Section: Discussionsupporting

confidence: 65%

“…This number was lower than that reported by Brito et al (2015), who observed a similar genus (Streptomyces) to those found for this study. The genus Streptomyces was previously reported by Silva et al (2013) in soils from the Brazilian Cerrado which is the most common in several regions (Anandan et al, 2016), with reports in the literature describing its occurrence in Atacama desert soils in Chile (Okoro et al, 2009), India (Kumar et al, 2012;Das et al, 2014) and Egypt (Mabrouk and Saleh, 2014).…”

Section: Discussionmentioning

confidence: 85%

See 1 more Smart Citation

Characterization of actinobacteria from the semiarid region, and their antagonistic effect on strains of rhizobia

Lima¹,

Martins²,

Siqueira³

et al. 2017

Afr. J. Biotechnol.

View full text Add to dashboard Cite

The actinobacteria are the most abundant bacterial group in the soil, where they present different characteristics and antagonistic effects on other microorganisms. The objectives of this study were to characterize and evaluate the antagonistic effect of actinobacteria from the semiarid region on rhizobia from the same climatic region. Tests were performed to observe chromogenic and micro-morphological characteristics, tolerance to pH levels of melanin production, and use of carbon sources. This occurred due to the use of modified CDA culture media and basal medium supplemented with specific carbon sources, as well as the use of Ral color chat to observe the colonies. The in vitro antagonism on rhizobia was evaluated in yeast malt agar (YMA) culture medium for the observation of inhibition halo by actinobacteria. We observed different characteristics in relation to the color of aerial mycelium and reverse pigments; tolerance to media with acid and alkaline pH; and production of melanoid pigment occurred only in three strains. The morphological characteristics were described for genera Streptomyces and Saccharothrix, later confirmed by molecular sequencing. These genera were generalists in use of carbon sources, showing their physiological versatility. Regarding antagonism, nine strains of the genus Streptomyces were able to inhibit in vitro strains of Rhizobium tropici and Bradyrhizobium yuanmingense also coming from semiarid soils. The antagonism between actinobacteria and rhizobia can directly affect the symbiosis between nitrogen-fixing bacteria and leguminous plants. These results are pioneering in observing antagonism of these species of rhizobia under the climate condition of the semiarid region, and may contribute to agricultural biotechnology.

show abstract

Section: Discussionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 85%

Characterization of actinobacteria from the semiarid region, and their antagonistic effect on strains of rhizobia

Lima¹,

Martins²,

Siqueira³

et al. 2017

Afr. J. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…The rRNA secondary structure and the restriction sites of Actinobacterium Loyola VAS 10 were predicted using Genebee and NEBCutter online tools respectively. It showed the free energy of the predicted structure to be 204.9 kkal/mol, threshold energy to be 4.0 by using Genebee and restriction site analysis showed GC 58% and AT 42% content 29 .…”

Section: Antibiotics Produced By Actinomycetesmentioning

confidence: 99%

Untitled

2016

IJPSR

View full text Add to dashboard Cite

ABSTRACT:The rhizosphere represents the thin layer of soil surrounding plant roots and the soil occupied by the roots, supports large active groups of microorganisms. The vast organic compounds (amino acids, sugars etc.) secreted by plant roots in the rhizosphere provide a food source for microorganisms increasing microbial biomass and their activity in the rhizosphere. Antibiotics are antimicrobial compounds produced by living microorganism as secondary metabolites. These compounds are used therapeutically and sometimes prophylactically in the control of infectious diseases. The isolation of antibiotics from microorganisms is relatively easy as compared to chemical synthesis of antimicrobial agents. The isolation of antibiotics from microorganisms improved the discovery of novel antibiotics that could act as better chemotherapeutic agents. With the increased population pressure, costs and side effects and the development of resistance of pathogens to drugs for infectious diseases, there is an urgent need to explore microbes for development of new antimicrobial metabolites. As microorganisms grow in unique and extreme habitats, they may have the capability to produce unique and unusual metabolites. So rhizospheric soil gives an excellent option as a source for search of some new alternative medicines. This review highlights the recent developments in the production of antimicrobial compounds from rhizospheric soil microflora.

show abstract

“…Filamentous actinomycetes are known to have the ability to produce a wide variety of secondary metabolites [3,4], many of which have useful applications in human and veterinary medicine and agriculture [5], such as enzyme immuno-modulators, antibiotics, insecticides, herbicides, and anticancer agents [6]. Among Actinomycetes, Streptomyces which has long been recognized as a major source of bioactive molecules covers around 70% of the total antibiotic products [7,8].…”

Section: Introductionmentioning

confidence: 99%

Chromatography-Based Partial Purification and Characterization of Antimicrobial Compound Isolated From a Terrestrial Streptomyces

Sudha

Vasudevan

2018

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Objectives: A potent Streptomyces strain isolated from garden soil samples of Sathyabama University, Chennai, and the characterization and its antimicrobial activity was reported previously. This report deals with partial purification and characterization of the partially purified antimicrobial compound by liquid chromatography-mass spectrum (LC-MS) method. Method:The crude extract was characterized by ultra violet-visible spectrum (UV) and Fourier transform infrared spectroscopy (FTIR) subsequently partial purification was done by thin-layer chromatography, and the bands were used for LC-MS analysis. The obtained mass spectrum was searched in NIST for species data by molecular weight. The compound was identified by comparing the mass spectrum with the known compounds mass spectrum having 98-100% identical. The biological activity of the identified compound was searched in PubChem. Results:The UV and FTIR spectrum indicate the presence of hydroxyl group, aliphatic chain, aromatic, aliphatic amines, and ethers in the crude extract. The LC-MS spectrum revealed the presence of 12 compounds in high intensity resembling antimicrobial compounds such as imidazole, N-trifluoroacetyl, cyclohexane, 2-nitro, and stigmasterol. Conclusion:The instrumental analysis shows the presence of 14 different compounds, and PubChem compound search shows these compounds having various biological activities including antibacterial, antifungal, anticancer, antitumor, and antidepressant. This report insists us further to evaluate the anticancer, antitumor, and antitrypanosomal activities of the partially purified compound.

show abstract

Antibacterial activity of some actinomycetes from Tamil Nadu, India

Abstract: The present study showed that among the isolated actinomycetes, Actinobacterium Loyola PBT VAS 10 (accession No. JF501398) showed good antibacterial activity against the tested bacteria.

Cited by 46 publications

References 29 publications

Characterization of actinobacteria from the semiarid region, and their antagonistic effect on strains of rhizobia

Characterization of actinobacteria from the semiarid region, and their antagonistic effect on strains of rhizobia

Untitled

Chromatography-Based Partial Purification and Characterization of Antimicrobial Compound Isolated From a Terrestrial Streptomyces

Contact Info

Product

Resources

About