Molecular structure of the human cytoplasmic beta-actin gene: interspecies homology of sequences in the introns.

Nakajima-Iijima, Sadayo; Hamada, Hiroshi; Reddy, P. Seshidhar; Kakunaga, Takeo

doi:10.1073/pnas.82.18.6133

Cited by 627 publications

(237 citation statements)

References 45 publications

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“…Total RNA was isolated from samples by using modified acid guanidinium-thiocyanatephenol-chloroform extraction procedures (Chomczynski & Sacchi 1987) ) and rat (M20133) ) androgen receptor, hu-man and rabbit (M12674, X03635, M11457) (Fuqua et al 1990;Greene et al 1986aGreene et al , 1986b and rat (Y00102) Koike et al 1987) estrogen receptor, human and rabbit (M15716) Misrahi et al 1987) and rat (S64044) (Park & Mayo 1991) progesterone receptor, and human and rabbit (M10277) (Nakajima-Iijima et al 1985) and rat b-actin (V01217, J00691) (Nudel et al 1983). The PCR conditions and number of cycles, which were selected after communication with Dr. Wilson (androgen receptor), computer analysis (Oligo 4.0 software, National Biosciences; and NCBI BLAST) and extensive experimentation, are shown in Table 2.…”

Section: Molecular Biological Proceduresmentioning

confidence: 99%

Identification of androgen, estrogen and progesterone receptor mRNAs in the eye

Wickham

Gao

Toda

et al. 2000

Acta Ophthalmologica Scandinavica

311

213

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ABSTRACT.Purpose: Previous research has demonstrated that sex steroids exert a significant influence on the structure and function of numerous ocular tissues. To begin to explore the underlying basis of this hormone action, we examined whether various anterior and posterior tissues of the eye contain androgen, estrogen and progesterone receptor mRNAs. Methods: Tissue samples were obtained from adult male and female rats, rabbits and humans, processed for the isolation of total RNA and analyzed by RT-PCR, agarose gel electrophoresis and Southern blot hybridization. All PCR amplifications included positive and negative controls. Results: Our findings showed that androgen, estrogen and/or progesterone receptor mRNAs are present in the lacrimal gland, lacrimal gland acinar epithelial cells, meibomian gland, lid, palpebral and bulbar conjunctivae, cornea, iris/ciliary body, lens, retina/uvea, retina/choroid and retinal pigment epithelial cells of rats, rabbits or humans.Conclusions: Our findings demonstrate that sex steroid receptor mRNAs exist in a variety of ocular tissues and suggest that these sites may represent target organs for androgens, estrogens and/or progestins.

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Section: Molecular Biological Proceduresmentioning

confidence: 99%

Identification of androgen, estrogen and progesterone receptor mRNAs in the eye

Wickham

Gao

Toda

et al. 2000

Acta Ophthalmologica Scandinavica

311

213

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“…Owing to this exon connection strategy, DNAase I treatment of the initial template had no effect on the resulting PCR products. To confirm the integrity of the RNA used to generate the cDNAs and to check for equivalent efficiency of amplification all RT-PCR experiments were performed using sets of primers specific for the f-actin as well as the glucose 6-phosphate dehydrogenase (G6PD) housekeeping genes (Persico et al, 1981;Nakajima-lijima et al, 1985;Adams et al, 1992), which are located on chromosomes X and 7 respectively. Both genes code for highly conserved proteins.…”

Section: Reverse Transcriptase-polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

Frequent reduction or loss of DCC gene expression in human osteosarcoma

Horstmann

Pösl²,

Rb³

et al. 1997

Br J Cancer

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Summary The 'deleted in colon carcinoma' (DCC) gene has been considered a candidate tumour-suppressor gene that encodes for a transmembrane protein with strong structural similarity to members of the superfamily of neural cell adhesion molecules. It has been mapped to the chromosomal region 18q21 .1 and it is implicated in cellular differentiation and developmental processes. In human osteosarcoma allelic loss frequently occurs on the long arm of chromosome 18, suggesting a possible involvement of the DCC gene in the pathogenesis of this tumour entity. In the present study the mRNA and protein expression and rearrangements at the DNA level of the DCC gene were addressed in 25 osteosarcomas and several tumour cell lines, including osteosarcoma-and colon carcinoma-derived cell lines. Using an reverse transcriphase polymerase chain reach in (RT-PCR)-based approach DCC expression was found to be lost or substantially reduced in 14 of 19 high-grade osteosarcomas, in three of six lower grade osteosarcomas and most of the tumour cell lines, in contrast to normally differentiated osteoblasts. Immunohistochemical studies on DCC protein expression of 14 selected tumours correlated well with the RT-PCR-based results. In view of the putative tumour-suppressor characteristics of the DCC gene its loss or reduction of expression could be a specific event in the development or progression of many high-grade osteosarcomas.

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“…Comparable RNA loading was confirmed using a human psuclin probe [9]. Cells were exposed to lOO#M quercetin and IOOyM sodium arsenitc 4 days after the tnnsfection because DNA tmnsfcction and glyccrol shock may cause some stresses.…”

Section: 2 S/or H/or Hydridimiort Und Riborrucleusc Protcctiorlmentioning

confidence: 99%

Quercetin, a bioflavonoid, inhibits the increase of human multidrug resistance gene (MDR1) expression caused by arsenite

et al. 1992

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Expression of the MDRl gene, which encodes P-glycoprotein, is increased under some stress conditions. WC have repotted that quercedn. a bioflavonoid, inhibits the expression of heat-shock proteins. WC have identified the effects of quercetin on the MDRl gene expression in the human hepatocarcinoma cells line. HcpG?. The increase of P-glycoprolein synthesis and MDA/ mRNA accumulation caused by exposurL 1~ arscnite were inhibited by quercetin. The CAT assay suggested that quercctin suppressed the transcriptional activation of the MDRl gene after exposure to arseniLe. Although many drugs that prevent the Pglycoprotcin fumion have been reporlcd, this is the first report to describe the inhibition of MDR! expression by u leagent.

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Molecular structure of the human cytoplasmic beta-actin gene: interspecies homology of sequences in the introns.

Cited by 627 publications

References 45 publications

Identification of androgen, estrogen and progesterone receptor mRNAs in the eye

Identification of androgen, estrogen and progesterone receptor mRNAs in the eye

Frequent reduction or loss of DCC gene expression in human osteosarcoma

Quercetin, a bioflavonoid, inhibits the increase of human multidrug resistance gene (MDR1) expression caused by arsenite

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