Molecular structure, expression patterns, and localization of the circadian transcription modulator CYCLE in the cricket, Dianemobius nigrofasciatus

Circadian Rhythms and Period Expression in the Hawaiian Cricket Genus Laupala

“…However, even before the first clock mutant was reported in D. melanogaster , researchers had been investigating daily variation and circadian rhythms in crickets (Nowosielski and Patton 1963; Nowosielski and Patton 1964; Alexander and Meral 1967; McFarlane 1968). The circadian mechanism in crickets appears to differ from that of Drosophila , both in the molecular basis of the endogenous clock as well as the localization of the circadian pacemaker neurons (Sokolove and Loher 1975; Abe et al 1997; Shao et al 2008a; Shao et al 2008b; Abdelsalam et al 2008; Lupien et al 2003; Shao et al 2006; Shiga et al 1999). Understanding both the behavioral variation in circadian rhythms among crickets and mechanisms underlying cricket circadian clocks will provide insight into the evolution and plasticity of circadian rhythm generation, as well as further our understanding of the molecular basis of circadian timing.…”

“…Given the commonly observed daily cycling in cricket song, circadian rhythms of crickets have been a frequent subject of study, from early behavioral and physiological studies (Nowosielski and Patton 1963; Loher 1972; Loher 1974; Sokolove 1975; Sokolove and Loher 1975; Wiedenmann and Loher 1984; Abe et al 1997) to more recent molecular analyses (Hassaneen et al 2011; Shao et al 2008a; Shao et al 2008b; Moriyama et al 2008; Abdelsalam et al 2008; Lupien et al 2003; Shao et al 2006; Danbara et al 2010). Like other organisms, crickets possess an endogenous circadian clock (Loher 1972; Loher 1974; Abe et al 1997); however, there are physiological differences in the clock of crickets relative to Drosophila .…”

“…Like other organisms, crickets possess an endogenous circadian clock (Loher 1972; Loher 1974; Abe et al 1997); however, there are physiological differences in the clock of crickets relative to Drosophila . For example, the daily variation in expression of circadian clock proteins, including PER, CLK, TIM, which occurs in Drosophila (Dunlap 1999; Hardin 2005; Yu and Hardin 2006; Rosato et al 2006), appears to be lacking in crickets (Shao et al 2008a; Shao et al 2008b; Lupien et al 2003; Shao et al 2006). Additionally, CLK is the only one of these proteins which has been observed to translocate into the nucleus in crickets (Shao et al 2008a).…”

“…For example, the daily variation in expression of circadian clock proteins, including PER, CLK, TIM, which occurs in Drosophila (Dunlap 1999; Hardin 2005; Yu and Hardin 2006; Rosato et al 2006), appears to be lacking in crickets (Shao et al 2008a; Shao et al 2008b; Lupien et al 2003; Shao et al 2006). Additionally, CLK is the only one of these proteins which has been observed to translocate into the nucleus in crickets (Shao et al 2008a). However, it was recently demonstrated that per transcript abundance undergoes daily variation in Gryllus bimaculatus and that inhibition of PER expression leads to arrhythmia (Moriyama et al 2009; Moriyama et al 2008).…”

Comparative analysis of circadian clock genes in insects

“…In the ground cricket, Dianemobius nigrofasciatus immunoreactivity to PER, DBT, CRY and CLK was observed predominantly in the optic lobes, but intriguingly CYC was not observed in the CLK expressing neurons in this region. CLK and CYC were also expressed in the pars intercerebralis and suboesophageal ganglion (Shao et al ., 2006, 2008a). In contrast, in another ground cricket, Allenomobius allardi , these clock proteins appear to be particularly enriched in the suboesophageal ganglion (Shao et al ., 2006, 2008b).…”