Molecular spherical nucleic acids

Li, Hui; Zhang, Bohan; Lu, Xueguang; Tan, Xuyu; Jia, Fei; Xiao, Yue; Cheng, Zehong; Li, Yang; Silva, Dagoberto O.; Schrekker, Henri Stephan; Zhang, Ke; Mirkin, Chad A.

doi:10.1073/pnas.1801836115

Cited by 126 publications

(129 citation statements)

References 50 publications

(42 reference statements)

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Section: Governing Factors Of the Cellular Uptake Of Dna Nanostructuresmentioning

confidence: 99%

“…The authors observed that DBCC–SNAs enter HeLa cells twice as much as LDBC–SNAs. In 2018, Li et al reported the synthesis of “molecular SNAs” with a total size of ≈20 nm and defined the number of oligonucleotides attached to the nanoparticle surface . The authors found that T 8 polyoctahedral silsesquioxane (POSS)‐scaffolded SNAs (with 8 strands per particle) enter MCF‐7 cells and SKOV3 ovarian cancer cells slightly less than buckyball (C 60 )‐scaffolded SNAs (with 12 strands per particle) (Figure G).…”

Section: Governing Factors Of the Cellular Uptake Of Dna Nanostructuresmentioning

confidence: 99%

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Progress toward Understanding the Interactions between DNA Nanostructures and the Cell

Chiu

Choi

2019

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Advances in DNA nanotechnology empower the programmable assembly of DNA building blocks (oligonucleotides and plasmids) into DNA nanostructures with precise architectural control. As DNA nanostructures are biocompatible and can naturally enter mammalian cells without the aid of transfection agents, they have found numerous biological or biomedical applications as delivery carriers of therapeutic and imaging cargoes into mammalian cells for at least a decade. Nevertheless, mechanistic studies on how DNA nanostructures interact with cells have remained limited and incomprehensive until 2–3 years ago. This Review presents the recent progress in elucidating the “cell–nano” interactions of DNA nanostructures, with an emphasis on three key classes of structures commonly utilized in intracellular applications: tile‐based structures, origami‐based structures, and nanoparticle‐templated structures. Structural parameters of DNA nanostructures and strategies of biochemical modification for promoting intracellular delivery are discussed. Biological mechanisms for cellular uptake, including specific pathways and receptors involved, are outlined. Routes of intracellular trafficking and degradation, together with strategies for re‐directing their trafficking, are delineated. This Review concludes with several aspects of the “bio–nano” interactions of DNA nanostructures that warrant future investigations.

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Section: Governing Factors Of the Cellular Uptake Of Dna Nanostructuresmentioning

confidence: 99%

Section: Governing Factors Of the Cellular Uptake Of Dna Nanostructuresmentioning

confidence: 99%

Progress toward Understanding the Interactions between DNA Nanostructures and the Cell

Chiu

Choi

2019

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“…For this purpose, the concept of spherical nucleic acids (SNA) is useful . With DNA densely functionalized on AuNPs (denser than that achieved on a planar surface), the face close to the surface can be used for conjugation, whereas the other face with exposed DNA is fully functional with all the merits of SNAs such as tighter binding of cDNA and sharp melting transitions (Figure a) ,. Herein, we communicate a general strategy to display functional SNAs on a diverse range of nanomaterials.…”

Section: Figurementioning

confidence: 99%

Polyvalent Spherical Nucleic Acids for Universal Display of Functional DNA with Ultrahigh Stability

2018

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Forn anomaterials that are difficult to functionalize by covalent attachment of DNA, we herein communicate ag eneral method taking advantage of the high avidity of polyvalent binding and the 3D structure of densely functionalized spherical nucleic acids (SNAs). Using DNA-functionalized gold nanoparticles,simple mixing leads to the formation of highly stable conjugates on 11 different materials including metals,m etal oxides,m etal-organic frameworks,t ransitionmetal dichalcogenides,n anocarbons,a nd polymers.T he adsorption affinity of SNAs can be over thousand-fold higher than that of free DNAo ft he same sequence,a nd practically irreversible conjugates are formed withstanding various denaturing agents.The surface attachment and molecular recognition functions of DNAa re spatially separated, showing ak ey advantage of SNAs.T he functionalized materials possess the properties of both the substrate and the SNA, allowing specific DNAh ybridization in buffer and in serum.

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“…Spherical nucleic acids (SNA) are polyvalent nanostructures, composed of a metal nanoparticle core with nucleic acids fragments attached to the surface [12]. Since then the technology of spherical nucleic acid has been expended into variety of structures [13]. Here, we review the current knowledge regarding arrangements involving metal nanoparticles and nucleic acids, as well as their potential biological applications.…”

Section: Introductionmentioning

confidence: 99%

Gold Nanoparticles in Conjunction with Nucleic Acids as a Modern Molecular System for Cellular Delivery

et al. 2020

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Development of nanotechnology has become prominent in many fields, such as medicine, electronics, production of materials, and modern drugs. Nanomaterials and nanoparticles have gained recognition owing to the unique biochemical and physical properties. Considering cellular application, it is speculated that nanoparticles can transfer through cell membranes following different routes exclusively owing to their size (up to 100 nm) and surface functionalities. Nanoparticles have capacity to enter cells by themselves but also to carry other molecules through the lipid bilayer. This quality has been utilized in cellular delivery of substances like small chemical drugs or nucleic acids. Different nanoparticles including lipids, silica, and metal nanoparticles have been exploited in conjugation with nucleic acids. However, the noble metal nanoparticles create an alternative, out of which gold nanoparticles (AuNP) are the most common. The hybrids of DNA or RNA and metal nanoparticles can be employed for functional assemblies for variety of applications in medicine, diagnostics or nano-electronics by means of biomarkers, specific imaging probes, or gene expression regulatory function. In this review, we focus on the conjugates of gold nanoparticles and nucleic acids in the view of their potential application for cellular delivery and biomedicine. This review covers the current advances in the nanotechnology of DNA and RNA-AuNP conjugates and their potential applications. We emphasize the crucial role of metal nanoparticles in the nanotechnology of nucleic acids and explore the role of such conjugates in the biological systems. Finally, mechanisms guiding the process of cellular intake, essential for delivery of modern therapeutics, will be discussed.

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Molecular spherical nucleic acids

Cited by 126 publications

References 50 publications

Progress toward Understanding the Interactions between DNA Nanostructures and the Cell

Progress toward Understanding the Interactions between DNA Nanostructures and the Cell

Polyvalent Spherical Nucleic Acids for Universal Display of Functional DNA with Ultrahigh Stability

Gold Nanoparticles in Conjunction with Nucleic Acids as a Modern Molecular System for Cellular Delivery

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