Polyvalent Spherical Nucleic Acids for Universal Display of Functional DNA with Ultrahigh Stability

Liu, Biwu; Huang, Zhicheng; Liu, Juewen

doi:10.1002/ange.201805532

Cited by 20 publications

(13 citation statements)

References 76 publications

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“…For divalent metal-mediated adsorption, when challenged by urea, the linear DNA was also desorbed more than the spherical DNA (Figure C) . This data also confirmed the importance of polyvalent hydrogen bonding on the adsorption affinity of spherical DNA on PDA.…”

Section: Resultsmentioning

confidence: 90%

“…For divalent metal-mediated adsorption, when challenged by urea, the linear DNA was also desorbed more than the spherical DNA (Figure 3C). 44 This data also confirmed the importance of polyvalent hydrogen bonding on the adsorption affinity of spherical DNA on PDA. The densely packed spherical DNAs are more rigid and would have a smaller loss in entropy upon adsorption, which are also favorable for adsorption.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Cooperative Metal Ion-Mediated Adsorption of Spherical Nucleic Acids with a Large Hysteresis

Zandieh

Liu

2020

Langmuir

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Spherical nucleic acids (SNA) refer to nanoparticles attached with a high density of oligonuleotides. Linear and spherical nucleic acids have many differences such as hybridization affinity, melting transition, and cellular uptake. In this work, these two types of DNA of the same sequence were compared for adsorption on polydopamine (PDA) nanoparticles and graphene oxide (GO). We focused on the effect of metal ions including Na + , Ca 2+ , and Zn 2+ since metal ions are indispensible for DNA adsorption on PDA and GO. Gold nanoparticles (AuNPs) of various sizes were used to prepare the SNAs. For both PDA and GO, a normal binding curve of one metal ion was obtained for adsorbing the linear DNA, while the spherical DNAs larger than 5 nm showed a sigmoidal binding curve requiring multiple metal ions. Urea and EDTA were used to probe DNA adsorption affinity, where the spherical DNA showed stronger adsorption in general. In the presence of 300 mM Na + , 4 M urea or 4 mM EDTA failed to desorb the 13 nm spherical DNA. The spherical DNA showed a very large hysteresis of metal-dependent adsorption. This study demonstrates another unique property of SNA compared to linear DNA, revealing interesting orientation and packing of DNA on AuNPs, which has deepened our understanding of DNA interface chemistry.

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Section: Resultsmentioning

confidence: 90%

Section: ■ Results and Discussionmentioning

confidence: 99%

Cooperative Metal Ion-Mediated Adsorption of Spherical Nucleic Acids with a Large Hysteresis

Zandieh

Liu

2020

Langmuir

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“…Gas Pressure-Based Detection of Cancer Cells. First, the thiolated MUC1 aptamer (S2.2) with an initial concentration of 6.4 μM was attached onto AuNSs using the salt-aging method, 34 and the excess free DNA was removed after centrifugation. Then, the cell media containing 10 μL S2.2-AuNSs (0.75 mg/mL) were incubated with different concentrations of MCF-7 cells (10 1 , 10 2 , 10 3 , 10 4 , 10 5 , and 10 6 cells/mL) for 3 h at 37 °C, respectively.…”

Section: Auns-mediatedmentioning

confidence: 99%

NIR Light-Responsive Hollow Porous Gold Nanospheres for Controllable Pressure-Based Sensing and Photothermal Therapy of Cancer Cells

Tong

Wang

et al. 2019

Anal. Chem.

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Pressure-based signal transduction has attracted recent and extensive attention due to its high sensitivity and simplicity. The most popular way to generate gas pressure relies on catalyst-mediated decomposition of H2O2. Despite its high sensitivity, this method lacks spatial and temporal control of the reaction, and may suffer from variations due to the dead time of mixing. In this work, we report a new reaction using near-infrared (NIR) light to heat hollow porous gold nanospheres (AuNSs) for thermal decomposition of NH4HCO3. Comparisons were made on these two systems especially on controlled pressure production. As an example of application, our light-controlled system was used for the detection of MCF-7 cancer cells by attaching the S2.2 aptamer on the AuNSs. The detection limit was as low as 2 cells/mL. Meanwhile, the heat produced by the AuNSs was used to induce localized hyperthermia at the surface of the cancer cells. This interesting theranostic system provides new insights into pressure-based sensing and may inspire new analytical applications.

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“…Interestingly, DNA-AuNPs can strongly adsorb on all these materials to indirectly attach DNA. 77 With a dense DNA layer, each DNA facing the material can interact strongly to rival covalent interactions taking advantage of the polyvalent binding effect, while the DNA strands away from the material are still functional.…”

Section: Conjugation Of Dna To Aunpsmentioning

confidence: 99%

Interface-Driven Hybrid Materials Based on DNA-Functionalized Gold Nanoparticles

Liu

Juewen

2019

Matter

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160

147

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DNA-functionalized gold nanoparticles (AuNPs) are popular hybrid materials with applications in directed assembly, biosensor development, and drug delivery. This system is particularly interesting due to the versatile optical and catalytic properties of AuNPs combined with the molecular recognition and programmable properties of DNA. Instead of emphasizing applications, this review focuses on the interfaces including adsorption of thiol and DNA bases, colloidal stability of AuNPs, and related methods for preparing this conjugate. The effects of salt, pH, proteins, and DNA base composition are discussed for controlling the conformation, density, and stability of DNA. Hybridization of DNA on AuNPs, DNA-directed growing of materials, and cellular uptake of this conjugate are discussed as examples to highlight the importance of the interfaces. Our understanding of these biointerfaces is still far from complete, and a few future research opportunities for engineering hybrid materials are described.

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Polyvalent Spherical Nucleic Acids for Universal Display of Functional DNA with Ultrahigh Stability

Cited by 20 publications

References 76 publications

Cooperative Metal Ion-Mediated Adsorption of Spherical Nucleic Acids with a Large Hysteresis

Cooperative Metal Ion-Mediated Adsorption of Spherical Nucleic Acids with a Large Hysteresis

NIR Light-Responsive Hollow Porous Gold Nanospheres for Controllable Pressure-Based Sensing and Photothermal Therapy of Cancer Cells

Interface-Driven Hybrid Materials Based on DNA-Functionalized Gold Nanoparticles

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