Molecular Size, Homogeneity, and Hydrodynamic Properties of Purified Staphylococcal Enterotoxin B^*

The staphylococcal enterotoxins

“…From the results that have been reported, it would appear that calculation from the amino acid composition according to the method of Schachman [38] may be more reliable than the values obtained by pycnometry. For enterotoxin B, the value of 0.726 reported by Bergdoll et al [9] and the value of 0.731 reported by Spero et al [43], both calculated from the respective amino acid compositions determined by the two groups are lower than the value, 0.743, obtained by pycnometry by Wagman et al [45]. Schantzl on rechecking the partial specific volume for enterotoxin B reports that the experimental value he obtained by pycnometry was 0.730 which is in agreement with the values calculated from the amino acid composition.…”

The Staphylococcal Enterotoxins

“…From the results that have been reported, it would appear that calculation from the amino acid composition according to the method of Schachman [38] may be more reliable than the values obtained by pycnometry. For enterotoxin B, the value of 0.726 reported by Bergdoll et al [9] and the value of 0.731 reported by Spero et al [43], both calculated from the respective amino acid compositions determined by the two groups are lower than the value, 0.743, obtained by pycnometry by Wagman et al [45]. Schantzl on rechecking the partial specific volume for enterotoxin B reports that the experimental value he obtained by pycnometry was 0.730 which is in agreement with the values calculated from the amino acid composition.…”

Design of Adsorptive Columns for Specific Pathogen Removal: Application to Staphylococcal Enterotoxin B

“…The particle effective diffusivity for SEB ( D p ) in Toyopearl beads has been determined experimentally to be 4.83 × 10 ‐ 11 m/s (17). Comparing with the free molecular diffusivity of SEB ( D m = 7.70× 10 ‐ 11 m/s) (23), the tortuosity for SEB in the resin is 1.59, a relatively small value probably due to the large (1000 Å) pores of the Toyopearl 650 M resin.…”