Enterotoxin C elaborated by Staphylococcus aureus strain 137 was purified by chromatography on a CM-cellulose column and gel filtration through Sephadex G-75 and G-50. The purified enterotoxin exhibited a high degree of homogeneity as determined by synthetic boundary spreading and by electrophoresis. This was confirmed by estimation of purity by the double-diffusion tube technique. The highly purified toxin is a simple, colorless, and antigenic protein with a sedimentation coefficient of 3.0 S. A molecular weight

Identification of a new enterotoxin was accomplished by purification of the enterotoxins produced by staphylococcal strains 137 and 361 and by the preparation of specific antitoxin to the enterotoxin. Toxicity of the preparations was determined in rhesus monkeys, and specificity of the enterotoxin-antitoxin reaction was determined in gel-diffusion plates. The enterotoxin has been designated enterotoxin C, and staphylococcal strain 137 (ATCC 19095) was selected as the prototype strain.

Identification of a new enterotoxin was accomplished by purification of the enterotoxin produced by staphylococcal strain FRI-326 and by preparation of specific antitoxin to the enterotoxin. Toxicity of the preparations was determined in rhesus monkeys, and specificity of the enterotoxin-antitoxin reaction was determined in gel diffusion plates. The enterotoxin was designated enterotoxin E.

The amino acid compositions of enterotoxin C strain 137 and enterotoxin C strain 361 were determined with a Spinco amino acid analyzer. Glutamic acid was determined as the N-terminal amino acid

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