Molecular signature of extended spectrum β-lactamase producing Klebsiella pneumoniae isolated from bovine milk in eastern and north-eastern India

Koovapra, S.; Bandyopadhyay, S.; Das, Gunjan; Bhattacharyya, Debasish; Banerjee, Jaydeep; Mahanti, Achintya; Samanta, Indranil; Nanda, Pramod Kumar; Kumar, Amod; Mukherjee, Reena; Dimri, Umesh; Singh, Raj Kumar

doi:10.1016/j.meegid.2016.07.032

Cited by 56 publications

(52 citation statements)

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“…Koovapra et al . () also observed that there was a clonal diversity of E. coli isolates from the same region and inter relationship in isolates of different region. It is believed that the wide spread use of the carbapenems in human medicine might have resulted in emergence of CRE and cross‐species transmission.…”

Section: Discussionmentioning

confidence: 78%

“…There is no systematic study on occurrence of carbapenem resistance in Enterobacteriaceae members in food animals from India (Koovapra et al . ). Hence, the present work was intended to investigate the occurrence and spatial epidemiology of carbapenem‐resistant E. coli isolates from 10 different organized farms in seven important pig breeding states of India and for further molecular characterization of the resistant isolates in relation to their virulence factors.…”

Section: Introductionmentioning

confidence: 97%

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Spatial molecular epidemiology of carbapenem-resistant and New Delhi metallo beta-lactamase (blaNDM)-producingEscherichia coliin the piglets of organized farms in India

et al. 2017

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Section: Discussionmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 97%

Spatial molecular epidemiology of carbapenem-resistant and New Delhi metallo beta-lactamase (blaNDM)-producingEscherichia coliin the piglets of organized farms in India

et al. 2017

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“…These strains usually produce extended spectrum β-lactamases and often confer resistance to almost all β-lactam antibiotics, including 3rd and 4th generation cephalosporins and other kinds of antibiotics (quinolones, trimethoprim/sulfamethoxazole, and aminoglycosides). The presence of such strains in food may represent a significant threat to consumers, as these pathogens have been recorded to cause obstinate infections with increased morbidity and mortality (Warjri et al, 2015 ; Koovapra et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and Genotypic Characterization of Klebsiella pneumoniae Isolated From Retail Foods in China

Zhang

Yang

et al. 2018

Front. Microbiol.

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Klebsiella pneumoniae is not only a major hospital-acquired pathogen but also an important food-borne pathogen that can cause septicaemia, liver abscesses, and diarrhea in humans. The phenotypic and genotypic characteristics of K. pneumoniae in retail foods have not been thoroughly investigated in China. The objective of this study was to characterize K. pneumoniae isolates through biotyping, serotyping, determination of virulence factors, antibiotic resistance testing, enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR), and (GTG)5-PCR molecular typing. From May 2013 to April 2014, a total of 61 K. pneumoniae isolates were collected from retail foods in China. Using API 20E test strips, five different biotype profiles were identified among these isolates. The majority of isolates belonged to biochemical profile “5215773” (50 isolates, 80.6%). The capsular serotypes of the 61 K. pneumoniae isolates and one reference strain were determined by PCR. Of the seven capsular serotypes tested, four different capsular serotypes were identified. Serotypes K1, K20, K57, and K2 were detected in two, three, two, and one isolates, respectively. Serotypes K3, K5, and K54 were not detected. The presence of 11 virulence genes was assessed by PCR. The most common virulence genes were fimH (85.5%), ureA (79.0%), wabG (77.4%), uge (56.5%), and kfuBC (29.0%). ERIC-PCR and (GTG)5-PCR molecular typing indicated high genetic diversity among K. pneumoniae isolates. We identified 60 different ERIC patterns and 56 distinct (GTG)5 patterns. Genotypic results indicated that isolates carrying similar virulence factors were generally genetically related. Some isolates from the same geographic area have a closer relationship. The isolates showed high levels of resistance to ampicillin (51/62, 82.2%). Resistance to streptomycin (11/62, 17.7%) and piperacillin (10/62, 16.1%) was also common. The presence of virulent and antibiotic-resistant K. pneumoniae in foods poses a potential health hazard for consumers. Our findings highlight the importance of surveillance of K. pneumoniae in foods.

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“…The second continent with the highest number of countries reporting E-ESBL in cattle is Asia, which shows description of E-ESBL in 13 countries (China, Hong Kong, India, Indonesia, Iran, Israel, Japan, Lebanon, Malaysia, Saudi Arabia, South Korea, Thailand and Taiwan), highlighting China and India which are the fourth and fifth largest cattle producers in the world (Adler et al, 2015;Ali et al, 2017;Barzan et al, 2017;Diab et al, 2016;Hassan et al, 2015;Hinthong et al, 2017;Kamaruzzaman, 2015;Koovapra et al, 2016;Ohnishi et al, 2013a;Su et al, 2016;Sudarwanto et al, 2016;Tark et al, 2017). They have different roles in the import and export scenario, as India is the world's largest exporter of cattle and China the largest importer, since its internal production is not sufficient for internal consumption (USDA et al, 2017).…”

Section: Asiamentioning

confidence: 99%

“…In India CTX-M-15, CTX-M-63, CTX-M, SHV-180, SHV and TEM in E-ESBL from cattle have already been described. Faecal origin and mastitis, being detected in E. coli and K. pneumoniae (Borah et al, 2014;Das et al, 2017;Koovapra et al, 2016). The presence of CTX-M-15 and SHV in E-ESBL in India has been reported in humans (Hawkey, 2008).…”

Section: Asiamentioning

confidence: 99%

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

Palmeira

Ferreira

2020

Heliyon

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Food producing animal is a global challenge in terms of antimicrobial resistance spread. Extended-spectrum betalactamase (ESBL)-producing Enterobacteriaceae are relevant opportunistic pathogens that may spread in many ecological niches of the One Health approach as human, animal and environment due to intestinal selection of antimicrobial resistant commensals in food production animals. Cattle production is a relevant ecological niche for selection of commensal bacteria with antimicrobial resistance from microbiota. Enterobacteriaceae show importance in terms of circulation of resistant-bacteria and antimicrobial resistance genes via food chain creating a resistance reservoir, setting up a threat for colonization of humans and consequent health risk. ESBL-producing Enterobacteriaceae are a threat in terms of human health responsible for life threatening outbreaks and silent enteric colonization of community populations namely the elder population. Food associated colonization is a risk difficult to handle and control. In a time of globalization of food trading, population intestinal colonization is a mirror of food production and in that sense this work aims to make a picture of ESBL-producing Enterobacteriaceae in animal production for food over the world in order to make some light in this reality of selection of resistant threats in food producing animal.

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Molecular signature of extended spectrum β-lactamase producing Klebsiella pneumoniae isolated from bovine milk in eastern and north-eastern India

Cited by 56 publications

References 44 publications

Spatial molecular epidemiology of carbapenem-resistant and New Delhi metallo beta-lactamase (blaNDM)-producingEscherichia coliin the piglets of organized farms in India

Spatial molecular epidemiology of carbapenem-resistant and New Delhi metallo beta-lactamase (blaNDM)-producingEscherichia coliin the piglets of organized farms in India

Phenotypic and Genotypic Characterization of Klebsiella pneumoniae Isolated From Retail Foods in China

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

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