2016
DOI: 10.1016/j.chemphyslip.2016.02.004
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Abstract: The role of membrane cholesterol as a crucial regulator in the structure and function of membrane proteins and receptors is well documented. However, there is a lack of consensus on the mechanism for such regulation. We have previously shown that the function of an important neuronal receptor, the serotonin1A receptor, is modulated by cholesterol in hippocampal membranes. With an overall objective of addressing the role of 3 membrane physical properties in receptor function, we measured the viscosity of hippoc… Show more

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Cited by 26 publications
(25 citation statements)
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References 59 publications
(25 reference statements)
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“…NaN 3 stops Type II oxidation path, which must otherwise assist Type I oxidation through peroxide formation. Nevertheless, a pure Type I oxidation (through peroxide formation followed by lipid cleavage) is able to proceed, which led to a decrease of BODIPY‐C 10 lifetime, as in the absence of NaN 3 .…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…NaN 3 stops Type II oxidation path, which must otherwise assist Type I oxidation through peroxide formation. Nevertheless, a pure Type I oxidation (through peroxide formation followed by lipid cleavage) is able to proceed, which led to a decrease of BODIPY‐C 10 lifetime, as in the absence of NaN 3 .…”
Section: Resultsmentioning
confidence: 99%
“…Consequently, a wide range of fluorescent probes suitable for probing multiple properties of lipid membranes was developed,13 including probes for sensing membrane potential and fluidity,14 for detecting lipid order in the outer lipid leaflet of the lipid bilayer15 and for sensing changes in the membrane during apoptosis 16. In this work, we utilized BODIPY‐C 10 17, 18 (Figure 1), a fluorophore that belongs to a group of dyes termed ‘molecular rotors’ that have viscosity‐dependent fluorescence quantum yields, lifetimes,19, 20 and depolarization 21, 22. When combined with fluorescence lifetime imaging microscopy (FLIM), molecular rotors can be used to obtain spatially resolved viscosity maps of microscopic objects,17, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40 as well as to observe dynamic change in viscosity during relevant processes of interest 37, 39, 41, 42.…”
Section: Introductionmentioning
confidence: 99%
“…It now appears that actin serves as a critical point of integration of receptor signaling such that changes in the cytoskeleton induced by one signal can readily influence the function of other receptors. Ligand binding as well as G protein coupling in GPCRs has been shown to be altered by indirect changes in physico-chemical properties space of the membrane (Prasad et al, 2009;Pal et al, 2016). Additionally, modulation of the GPCR association has been reported to be directly linked to indirect effects Pawar, Prasanna and Sengupta, 2015).…”
Section: Conclusion and Future Perspectivesmentioning
confidence: 99%
“…4,4-Difluoro-4-bora-3a,4a-diaza-s-indacenes (BODIPYs) are a popular class of fluorescent probes that is characterized by high extinction coefficients, quantum yields and photostability 15 . BODIPY-based molecular rotors have been successfully used in the past for quantitative viscosity imaging in 2D and 3D cell culture 16 and in vivo 17 and can be targeted to the plasma membranes 10 12 .…”
Section: Introductionmentioning
confidence: 99%
“…FCS, FRAP). We have previously successfully applied FLIM with molecular rotors to measure the plasma membrane viscosity, using the rotors that can be targeted to the plasma membrane 10-12 . 4,4-Difluoro-4-bora-3a,4a-diaza-s-indacenes (BODIPYs) are a popular class of fluorescent probes that is characterized by high extinction coefficients, quantum yields and photostability 15 . BODIPY-based molecular rotors have been successfully used in the past for quantitative viscosity imaging in 2D and 3D cell culture 16 and in vivo 17 and can be targeted to the plasma membranes [10][11][12] .…”
mentioning
confidence: 99%