2020
DOI: 10.1038/s41598-020-70972-5
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: Membrane fluidity plays an important role in many cell functions such as cell adhesion, and migration. In stem cell lines membrane fluidity may play a role in differentiation. Here we report the use of viscosity-sensitive fluorophores based on a BODIPY core, termed "molecular rotors", in combination with Fluorescence Lifetime Imaging Microscopy, for monitoring of plasma membrane viscosity changes in mesenchymal stem cells (MSCs) during osteogenic and chondrogenic differentiation. In order to correlate the visc… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
4

Citation Types

0
25
0

Year Published

2020
2020
2023
2023

Publication Types

Select...
9

Relationship

3
6

Authors

Journals

citations
Cited by 37 publications
(25 citation statements)
references
References 38 publications
0
25
0
Order By: Relevance
“… 3 The complex interplay between all these factors allow living cells to maintain membrane viscosity within narrow limits, specific to each cell type, which is key to cell homeostasis and survival. 3 7 …”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“… 3 The complex interplay between all these factors allow living cells to maintain membrane viscosity within narrow limits, specific to each cell type, which is key to cell homeostasis and survival. 3 7 …”
Section: Introductionmentioning
confidence: 99%
“…3 The complex interplay between all these factors allow living cells to maintain membrane viscosity within narrow limits, specific to each cell type, which is key to cell homeostasis and survival. [3][4][5][6][7] Chemotherapy remains one of the main types of treatment for cancer. However, the resistance of tumor cells to chemotherapeutic drugs presents a serious and unsolved problem limiting the efficacy of the therapy.…”
Section: Introductionmentioning
confidence: 99%
“…Thus, the excitation maximum of flippers is red-shifted in solid-ordered (S o ) and liquid-ordered (L o ) compared to liquid-disordered (L d ) phases of membranes. In contrast, the emission maximum remains basically unchanged because flippers always emit from the planar form. , This mode of action of flipper probes, operating under equilibrium conditions in the ground state, , is distinct from most other membrane probes that function off-equilibrium in the excited state. Flipper probes thus report on pressure from the surrounding confined space, and not on viscosity. , …”
Section: Introductionmentioning
confidence: 97%
“…27,28,29 Apart from the molecular machine aspects of rotors, there already exist several applications of molecular rotors as probes of local conditions in biological systems, or as sensors. [30][31][32] Rotor activity signified by variation in fluorescence output wavelength of certain molecule types can be harnessed to reveal local viscosity in biological membranes or models thereof, [33][34][35][36] while rotation rate modulation also signalled by fluorescence emission characteristics can also be used to indicate the presence of analytes. [37][38][39] Molecules having several proximal component moieties are suitable archetypes of rotors because of intramolecular interactions that govern the mutual motion of substituents.…”
Section: Toc Graphic Introductionmentioning
confidence: 99%