Molecular profiling using RAPD technique of salt and drought tolerant regenerants of sugarcane

Characterization of drought-tolerant sugar beet mutants induced with gamma radiation using biochemical analysis and isozyme variations

“…It is vital for any breeding program to have sufficient genetic variation in a population to allow for the production of new genotypes for the purpose of improving crops against abiotic and biotic stress conditions or increasing crop productivity and quality . The cellular and molecular bases of genetic variations are mitotic irregularities leading to chromosomal instability, which occurs as a result of gene amplification, deletion, point mutation, inactivation or reactivation of silent genes, transposition, somatic crossover and DNA methylation in the case of epigenetic variation . Ionizing radiations such as gamma rays can generate many kinds of DNA mutations ranging from point mutation to chromosome aberration.…”

“…Ionizing radiations such as gamma rays can generate many kinds of DNA mutations ranging from point mutation to chromosome aberration. Therefore they are frequently used in breeding programs to increase genetic variations in populations . The first step of radiation mutagenesis under tissue culture conditions determines the optimal radiation dose(s).…”

“…In vitro culture techniques are ideal for studying stress tolerance mechanisms, because they allow relatively rapid responses, short generation times and use of controlled environments. Additionally, the combination of plant tissue culture and mutation induction techniques accelerates the breeding process, thus leading to a more economical output …”

Assessment of Genetic Polymorphism in Sugarcane Somaclones by Using Random Amplified Polymorphic DNA

“…The most commonly applied molecular markers used to study polymorphism are random amplified polymorphic DNA (RAPDs) and simple sequence repeat (SSRs) which were used to measure the variability present in the somaclones (Yadav et al, 2006;Lal et al, 2008;Shahid et al, 2012;Sobakumari, 2012). The main advantage of RAPDs is that they are quick and easy to assay requiring low quantities of template DNA.…”

Drought Resistant Selection on Soybean Somaclonal Variants

“…In vitro selection technique has been used to improve tolerance to drought stress for maize (Matheka et al, 2008), wheat (Almansouri et al, 2001, and sugarcane (Yadav et al, 2006). In vitro selection for tolerance to drought is usually done by culturing cells/callus/tissues on selective media containing polyethylene glycol (PEG, HO (CH2-CH2) n-CH2-CH2OH, n ≥ 4).…”