Drought Resistant Selection on Soybean Somaclonal Variants

Abstract

This research was conducted to evaluate the yield potential of 19 somaclonal variants resulting from in vitro selection when planted under drought stress condition in the field. Field test was done by planting the variants, the parents, and checked varieties in the field during dry season, and was irrigated once a week for non-stress and once two weeks for drought stress treatment. Split-plot design arranged in a factorial (2 x 28) with three replications was used in this research. Observations were done on yield and yield components. Analysis of variance was used to see the difference between treatments and then it was continued with analysis using Honestly Significant Difference test to find out the best treatments. There was no interaction between genotype and drought stress on seed yield. Different genotypes showed a significant difference on this character. It indicated that the yield potential of selected variants was not affected by drought stress treatment. This research gave 10 variants having the potential to be developed as drought resistant genotypes. However, these ten potential genotypes need to be tested further in field trial to find out the yield adaptability and stability and their resistance to drought stress.

Evaluation of Tef (Eragrostis tef (Zucc.) Trotter) Somaclones for Drought Tolerance

“…The somaclones, Pop12S20.5 and Melko0 produced minimum grain yields of 1.74 and 3.14 g/pot under moisture stress and non-stress conditions, respectively (Table 2). Saxena et al [32] in pigeonpea, Widoretno et al [33] in soya bean and Zarif et al [21] in sorghum reported superiority of the somaclones over the donor parents for grain yield. On the contrary, Carver and Johnson [34] and Cheng et al [35] both in winter wheat reported grain yield in somaclones were low as compared to the parents.…”

“…Somaclonal variants of wheat with increased drought tolerance than their donor parents (non-tissue cultured plants) have been identified in pots under greenhouse [25]. The study of Widoretno [33] on soybean and Hemon and Sudarsono [24] on peanut also reported that the increase in drought tolerance of the somaclonal variants than the parents. In the current study twelve tef somaclones (R1 generation) derived from R0 generation and three parental genotypes were evaluated under moisture stress and non-stress conditions.…”

Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms

“…Moreover, disease resistance mutants have been regenerated through induced mutation in other plants such as abaca, banana, coffee and peanut (Yusnita et al 2005;Ibrahim et al 2018;Indrayanti et al 2018;Li et al 2019). Furthermore, irradiation may be employed to augment variation among regenerated plantlets (Jain 2005; and yielded abiotic stresstolerant (Widoretno et al 2003;Penna et al 2012;Widoretno et al 2012;Oladosu et al 2015;Rahayu and Sudarsono 2015;Suprasanna et al 2016) and biotic stress resistant (Yusnita et al 2005;Rai et al 2011;Sutanto et al 2014;Indrayanti et al 2018) mutant lines. Therefore, somaclonal variation and induced mutation by gamma irradiation might also be used as an alternative approach to generating soft-rot disease resistance P. amabilis mutants.…”