Molecular profiling of intrabony defects' gingival crevicular fluid

Abstract

Periodontitis is a chronic inflammatory disease attributed to a change in the periodontal microbiota toward dysbiosis, resulting in destruction of the periodontium and eventually tooth loss. 1 As periodontitis progresses, it results in intrabony periodontal osseous defects that are associated with an increased probability of tooth loss. 2 Gingival crevicular fluid (GCF) is both a physiologic fluid and an inflammatory exudate. 3 With the establishment of periodontitis, GCF increases 5.5-fold 4 and becomes enriched by host and bacterial

Molecular profiling of gingival crevicular fluid fails to distinguish between infrabony and suprabony periodontal defects

“…Conversely, the residual lateral walls of infrabony defects may help stabilize the blood clot even in the absence of specific regenerative mediators (Warrer & Karring, 1992). In contrast with previous studies (Koidou et al, 2022; Wikesjö et al, 1994) speculating that intrabony defects offered a unique favourable molecular microenvironment compared with suprabony defects, the present results have shown no difference for the markers included in the present analysis between infrabony and suprabony defects in patients with untreated periodontitis.…”

“…Moreover, our results are in contrast with a previous investigation (Koidou et al, 2022) reporting no significant difference in levels of BMP-2, EGF, SOST and PDGF-AA between infrabony defects and healthy sites. This difference may be ascribed to the smaller sample size adopted in the previous study (Koidou et al, 2022). However, in agreement with the above authors, the present investigation reported no significant difference for RANKL, OPN, PDGF-BB and TIMP-1 between diseased sites and healthy controls.…”

“…Each marker level was reported as pg/mL. The 18 GCF markers were (Koidou et al, 2022;Koidou, Cavalli, et al, 2020;Koidou, Chatzopoulos, et al, 2020).…”

“…Previous data have shown how the inflammatory markers identified in patients with chronic periodontitis reflect the generalized inflammatory state rather than diseased sites only (Zekeridou et al, 2017). Other authors reported how infrabony defects differ from healthy sites for the GCF profile, demonstrating significantly increased concentrations of inflammatory and connective tissue degradation markers, as well as of markers of regeneration (Koidou et al, 2022).…”

Matrix Metalloproteinase 9 (MMP-9) and Interleukin-8 (IL-8) in Gingival Crevicular Fluid after Regenerative Therapy in Periodontal Intrabony Defects with and without Systemic Antibiotics—Randomized Clinical Trial

“…Recently, a work concerning the molecular profiling of intrabony defects was published. Intrabony defects presented significantly increased GCF volume as well as increased IL-1 alpha, IL-1 beta, IL-6, IFN gamma and MMP-8 levels compared to periodontally healthy sites [ 45 ]. The use of local biomarkers offer the possibility for real-time assessment of the healing process.…”