Molecular profiling of human oocytes after vitrification strongly suggests that they are biologically comparable with freshly isolated gametes

Pietro, Cinzia Di; Vento, Marilena; Guglielmino, Maria Rosa; Borzì, Placido; Santonocito, Manuela; Ragusa, Marco; Barbagallo, Davide; Duro, Laura R.; Majorana, Alessandra; Palma, Adele De; Minutolo, Elisa; Scollo, Paolo

doi:10.1016/j.fertnstert.2010.04.060

Cited by 35 publications

(20 citation statements)

References 25 publications

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“…34 MiRNAs, more than mRNAs and proteins, represent biological molecules to be used to investigate human pathologies. 35,36 Particular features, such as high stability, strength to mechanical or biochemical degradation and easy detection, made circulating miRNAs excellent candidates to discriminate the state of health in cancers, degenerative pathologies, gynecological, and infectious diseases. 28,[37][38][39] In spite of their high potentiality, their low specificity could be a problem.…”

Section: Discussionmentioning

confidence: 99%

MiR‐27a‐3p and miR‐124‐3p, upregulated in endometrium and serum from women affected by Chronic Endometritis, are new potential molecular markers of endometrial receptivity

Pietro

Caruso

Battaglia

et al. 2018

American J Rep Immunol

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Section: Discussionmentioning

confidence: 99%

MiR‐27a‐3p and miR‐124‐3p, upregulated in endometrium and serum from women affected by Chronic Endometritis, are new potential molecular markers of endometrial receptivity

Pietro

Caruso

Battaglia

et al. 2018

American J Rep Immunol

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“…We applied our previously published protocol for human oocyte RNA isolation and RT-PCR reactions. 26 We performed five different PCR reactions per single oocyte and repeated the same analysis in every sample. Primer sequences and RT-PCR condition are reported in Table S1.…”

Section: O N O T D I S T R I B U T Ementioning

confidence: 99%

TAp73 is downregulated in oocytes from women of advanced reproductive age

et al. 2011

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Studies on oocyte transcriptome are important to understand the biological pathways involved in oogenesis, totipotence and early embryonic development. Moreover, genes regulating physiological pathways in gametes could represent potential candidates for reproductive disorders. In addition to oocyte specific transcription factors, also the members of the p53 family could be etiologically involved due to their biological functions. In fact, their role in the control of cell cycle, apoptosis and germ-line genome stability is well known. Female reproductive aging is one of the causes of fertility reduction and it is often associated with egg aneuploidy increase. In order to verify the potential involvement of p73 in reproductive aging, we determined its expression in single mature MII oocytes from two groups of women, younger than 35 or older than 38 y, respectively. We found that TAp73 isoforms are downregulated in oocytes from women older than 38 y. We confirmed these data in pools of mouse oocytes. TAp73 downregulation in oocytes from women of advanced reproductive age could explain both the reduction of fertility and the increase of newborns with chromosomal abnormalities.

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“…Pietro et al studied the biomolecular quality of vitrified oocytes. They compared eight genes involved in embryo development and the transcriptome and found no differences between the fresh and vitrified oocytes [36]. In a report based on over 900 births following the use of frozen-thawed oocytes by Noyes et al a congenital anomaly rate of 1.3 % was found which is not different from the rate observed in natural conceptions [37].…”

Section: Assisted Reproductive Technology-oocyte Cryopreservationmentioning

confidence: 95%

Fertility Preservation in Reproductive Age Women with Cancer

Kovács

2014

J Obstet Gynecol India

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Cancer may be detected at any age and could affect children, and reproductive age women as well. In recent years, cancer treatment has become less destructive and more specific. As a result, survival rates and quality of life following successful treatment have continuously improved. Cancer treatment typically involves surgery, chemo- or radiation therapy, or the combinations of these. These interventions often adversely affect the function of the reproductive organs. Chemo- and radiation therapy are known to be gonadotoxic. Survivors of oncologic therapy are typically rendered infertile primarily due to the loss of ovarian function. There are, however, several medical, surgical, and assisted reproductive technology options that could be and should be offered to those diagnosed with cancer and wish to maintain their fertility. Embryo cryopreservation has been available for decades and has been successfully applied for fertility preservation in women diagnosed with cancer. Recent advances in cryobiology have increased the efficacy of not just embryo but even oocyte and ovarian tissue freezing-thawing. Oocyte vitrification just like embryo cryopreservation requires the use of stimulation but does not require the patient to be in a stable relationship or accept the use of donor sperm. Ovarian tissue cryopreservation does not require stimulation and, following successful transplantation, provides the patient with the most eggs but is currently still considered experimental. This paper summarizes the various fertility-sparing medical, surgical and assisted reproductive technology options. It reviews the current status of embryo, oocyte, and ovarian tissue cryopreservation and discusses their risks and benefits.

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Molecular profiling of human oocytes after vitrification strongly suggests that they are biologically comparable with freshly isolated gametes

Cited by 35 publications

References 25 publications

MiR‐27a‐3p and miR‐124‐3p, upregulated in endometrium and serum from women affected by Chronic Endometritis, are new potential molecular markers of endometrial receptivity

MiR‐27a‐3p and miR‐124‐3p, upregulated in endometrium and serum from women affected by Chronic Endometritis, are new potential molecular markers of endometrial receptivity

TAp73 is downregulated in oocytes from women of advanced reproductive age

Fertility Preservation in Reproductive Age Women with Cancer

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