Molecular prevalence of <i>Theileria</i> infections in cattle in Yanbian, north-eastern China

Jia, Lijun; Zhao, Shaowei; Xie, Suzhu; Li, Hang; Wang, Hao; Zhang, Shuang

doi:10.1051/parasite/2020017

Cited by 10 publications

(5 citation statements)

References 14 publications

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“…Theileria orientalis types 1, 3, 4, 5, 6, 7, N2, and N3 were reported in cattle and buffalo using the merozoite surface antigen ( MPSP ) gene [ 6 , 7 ]. The clinical signs in infected animals included fever, anemia, jaundice, superficial lymph node enlargement, lethargy, lack of appetite, and exercise intolerance [ 8 ]. Theileriosis may cause a high ratio of mortality in pregnant cows and small ruminants [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Theileria infection in bullfighting cattle in Thailand

et al. 2022

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Background and Aim: An apicomplexan protozoan parasite, namely, Theileria, primarily causes theileriosis in cattle worldwide. The virulence of the disease has been neglected because of it's low pathogenicity. However, the disease can have a substantial effect, depending on the virulence of the species, low host immunity, and coinfection. In Thailand, the molecular detection of Theileria infection in bullfighting cattle and its hematological alterations have not been reported. Thus, this study aimed to identify Theileria species in bullfighting cattle in Thailand. Materials and Methods: Blood samples were collected from bullfighting cattle presented at the Prince of Songkla University Animal Hospital and were determined on the basis of hematological evaluation and DNA extraction. Molecular detection using the 18s rRNA and merozoite surface antigen genes was conducted for Theileria spp. and Theileria orientalis, respectively. In addition, bidirectional sequencing of the positive samples was performed. Hematological alterations between Theileria infected and uninfected groups were statistically evaluated. Results: The levels of Theileria spp. and T. orientalis infection in bullfighting cattle were 44.62% (58/130) and 41.54% (54/130), respectively. Theileria orientalis, Theileria sinensis, and Theileria spp. infections were identified in bullfighting cattle samples. Hematological evaluation indicated that the red blood cell (RBC) level was significantly lower in Theileria-infected cattle. Conclusion: This study was the first to use molecular techniques in the identification of Theileria infection in bullfighting cattle in Thailand, with nearly one-half of the study population infected. Theileria infection in bullfighting cattle altered the RBC level, resulting in anemia. Therefore, tick control measures should be promoted.

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Section: Introductionmentioning

confidence: 99%

Theileria infection in bullfighting cattle in Thailand

et al. 2022

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“…sinensis is also regarded as a cause of bovine theileriosis in China, and the pathogenicity of the parasite requires further research [9]. It was first isolated in Gansu province, China, and is mainly distributed in Asia [10,11]. It principally infects cattle, yaks and water buffalo and is transmitted by Haemaphysalis qinghaiensis [9,12,13].…”

Section: Introductionmentioning

confidence: 99%

The Common Occurrence of Theileria ovis in Tibetan Sheep and the First Report of Theileria sinensis in Yaks from Southern Qinghai, China

Wang

Zhang

et al. 2021

Acta Parasit.

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Purpose The tick-borne protozoa piroplasms, including Theileria and Babesia, are the cause of substantial economic losses to the livestock industry. However, in southern Qinghai province, China, there are limited information on the molecular characteristics of piroplasms. This study therefore aimed at determining the prevalence and genetic diversity of piroplasms. Methods In order to detect piroplasms, we examined 330 yaks and 236 Tibetan sheep blood samples by nested PCR. The differences in piroplasms prevalence in relation to different risk factors was analyzed using SPSS 26. Phylogenetic analysis based on 18S rRNA sequences was inferred using MEGA 7. Results During this study, Theileria spp. were detected in 33.6% (111/330) of yaks and 94.1% (222/236) of Tibetan sheep, but no Babesia was identified. Importantly, a comparison study revealed that T. ovis infection was highly prevalent in sheep (94.1%) but infrequent in yaks (6.1%), while T. sinensis was host-specific to yaks with an infection rate of 27.6%. In addition, male animals were more likely to be infected by T. sinensis and female animals were more likely to be infected by T. ovis. And animals below 4000 m areas reported a higher infection rate with T. sinensis (26.1% vs. 2.9%, p < 0.001). Alongside these differences in prevalence, we found a significantly higher T. sinensis infection rate in separated-grazing livestock (22.2% vs. 3.7%, p < 0.001), while mixed-grazing ruminants had a higher T. ovis infection rate (50.0% vs. 39.0%, p = 0.014). Furthermore, sequence analysis revealed that the 18S rRNA sequences obtained in this study shared 86.9-100.0% identities with each other and they were clustered into T. sinensis or T. ovis. Conclusion To our knowledge, this is the first report of T. sinensis in Qinghai region. In addition, high prevalence of the generally sub-clinical T. ovis in sheep indicates extensive exposure to ticks and transmission of tick-borne pathogens with a significant economic impact. This study provides insights into the distribution and genetic diversity of Theileria in China.

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“…T. orientalis belongs to a comparatively benign group of Theileria with universal existence. It causes little pathogenicity in bovine compared with T. parva and T. annulata; however, it has caused considerable economic loss in China [ 7 ], South Korea [ 8 , 9 , 10 , 11 ], Japan [ 12 ], Australia [ 6 ], and Vietnam [ 13 ] due to decreased production of meat, milk, and other by-products.…”

Section: Introductionmentioning

confidence: 99%

“…The active areas and prevalence of ticks are important factors in the epidemiology of Theileria species in cattle [ 7 ]. In South Korea, Haemaphysalis longicornis , which is the most prevalent tick species, is a primary vector of T. orientalis [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of Bovine Hemoprotozoa in South Korea

Kwak

Seo

2020

Pathogens

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Tick-borne pathogens cause economically significant diseases in cattle. Theileria spp. are parasitic protozoa and the causative agent of bovine theileriosis. Here we report the distribution and risk factors of bovine Theileria using blood samples taken between 2018 and 2019. Of 737 tested cattle, nine animals (1.2%) were positive for Theileria orientalis infection by 18S rRNA gene amplification. Further analysis of the infected samples using the T. orientalis major piroplasm surface protein (MPSP) gene revealed five different genotypes circulating in the population: Types 1, 2, 3, 7, and N3. To the best of our knowledge, this is the first research to describe the existence of the T. orientalis MPSP genotype N3 in South Korea. Although the prevalence of bovine T. orientalis was low, our study offers data on the geographical distribution and prevalence of bovine Theileria spp. in South Korea. Further studies are warranted to determine the correlation of clinical symptoms with parasite MPSP genotypes. Our data provide epidemiological information to help control bovine theileriosis in South Korea.

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Molecular prevalence of Theileria infections in cattle in Yanbian, north-eastern China

Cited by 10 publications

References 14 publications

Theileria infection in bullfighting cattle in Thailand

Theileria infection in bullfighting cattle in Thailand

The Common Occurrence of Theileria ovis in Tibetan Sheep and the First Report of Theileria sinensis in Yaks from Southern Qinghai, China

Genetic Diversity of Bovine Hemoprotozoa in South Korea

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