Molecular physiology reveals ammonium uptake and related gene expression in the seagrass Zostera muelleri

Pernice, Mathieu; Sinutok, Sutinee; Sablok, Gaurav; Commault, Audrey S.; Schliep, Martin; Macreadie, Peter I.; Rasheed, Michael A.; Ralph, Peter J.

doi:10.1016/j.marenvres.2016.10.003

Cited by 20 publications

(11 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Zostera muelleri GOIs were newly designed using Z. muelleri database from AquaticPlantsDB R (Sablok et al, 2018), 2 while housekeeping genes (HKGs) were taken from previous studies (Schliep et al, 2015;Pernice et al, 2016;Kim et al, 2018). For P. australis, however, no molecular resources are available to date, thus selected GOIs and HKGs were either newly designed or taken from previous studies on the congeneric species P. oceanica.…”

Section: Primer Designmentioning

confidence: 99%

Stress Memory in Seagrasses: First Insight Into the Effects of Thermal Priming and the Role of Epigenetic Modifications

et al. 2020

Self Cite

View full text Add to dashboard Cite

While thermal priming and the relative role of epigenetic modifications have been widely studied in terrestrial plants, their roles remain unexplored in seagrasses so far. Here, we experimentally compared the ability of two different functional types of seagrass species, dominant in the Southern hemisphere, climax species Posidonia australis and pioneer species Zostera muelleri, to acquire thermal-stress memory to better survive successive stressful thermal events. To this end, a two-heatwave experimental design was conducted in a mesocosm setup. Findings across levels of biological organization including the molecular (gene expression), physiological (photosynthetic performances and pigments content) and organismal (growth) levels provided the first evidence of thermal priming in seagrasses. Non-preheated plants suffered a significant reduction in photosynthetic capacity, leaf growth and chlorophyll a content, while preheated plants were able to cope better with the recurrent stressful event. Gene expression results demonstrated significant regulation of methylation-related genes in response to thermal stress, suggesting that epigenetic modifications could play a central role in seagrass thermal stress memory. In addition, we revealed some interspecific differences in thermal responses between the two different functional types of seagrass species. These results provide the first insights into thermal priming and relative epigenetic modifications in seagrasses paving the way for more comprehensive forecasting and management of thermal stress in these marine foundation species in an era of rapid environmental change.

show abstract

Section: Primer Designmentioning

confidence: 99%

Stress Memory in Seagrasses: First Insight Into the Effects of Thermal Priming and the Role of Epigenetic Modifications

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…stress related genes as part of plant stress response (Kan et al, 2017). It has been suggested that regulation of genes in this pathway could be used as an indicator for exposure to ammonium pulses in seagrass (Pernice et al, 2016). Passive ammonium transport is not regulated (Touchette & Burkholder, 2000), therefore uptake of ammonium by the plant continues.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Metabolomics and traditional indicators unveil stress of a seagrass (Cymodocea nodosa) meadow at intermediate distance from a fish farm

Kock

Hasler-Sheetal

Holmer

et al. 2020

Ecological Indicators

View full text Add to dashboard Cite

Seasonal variation of structural, physiological and growth indicators and the metabolome of the seagrass Cymodocea nodosa, as well as biogeochemical conditions of underlying sediment were studied in two meadows growing at increasing distance downstream from a fish farm in the Aegean Sea in order to assess seagrass performance under stress. Horizontal rhizome production decreased significantly with proximity to the fish farm (0.67 and 1.57 g DW m-2 d-ACCEPTED MANUSCRIPT 1 close and far from the fish-farm, respectively). This coincided with observed effects on ecophyiological indicators, such as rhizome nitrogen, leaf carbon and leaf δ 13 C, which were elevated with proximity to the fish-farm. Seasonality was shown by some indicators being elevated in either in the warm (C of all tissues and leaf δ 34 S) or the cold period (N of all tissues). Growth promoting metabolites (sucrose, fructose, myo-inositol, heptacosane, tetracosane, stigmasterol, catechin and alpha-tocopherol) were lower close to the zone, whereas metabolites involved with stress-response (alanine, serine, proline, putrescine, ornithine, 3,4dihydroxybenzoic acid and cinnamic acid) were higher. We found that growth-promoting metabolites were positively correlated with horizontal rhizome production, whereas the metabolites related to stress were negatively correlated. Metabolomic fingerprinting of seagrass provides opportunities for early detection of environmental degradation in marine ecological studies.

show abstract

“…While the catalase gene has been reduced to a single copy in Z. marina (possibly due to reduced xylemcharacteristics of submerged plants), whilst all three types of superoxide dismutase remain (Olsen et al, 2016 ). Glutathione-related transcripts have been isolated and profiled in P. oceanica, Z. muelleri , and Z. marina (Massa et al, 2011 ; Lauritano et al, 2015 ; Olsen et al, 2016 ; Pernice et al, 2016 ). In protein, enzymes related to cellular stress and photosynthesis genes in which the studies of seagrass in response to differing light conditions also revealed the differential expression of genes for (Dattolo et al, 2013 , 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Which Genes in a Typical Intertidal Seagrass (Zostera japonica) Indicate Copper-, Lead-, and Cadmium Pollution?

et al. 2018

View full text Add to dashboard Cite

Healthy seagrasses are considered a prime indicator of estuarine and coastal ecosystem function; however, as the only group of flowering plants recolonizing the sea, seagrasses are frequently exposed to anthropogenic heavy metal pollutants, which are associated with high levels of molecular damage. To determine whether biologically relevant concentrations of heavy metals cause systematic alterations in RNA expression patterns, we performed a gene expression study using transcriptome analyses (RNA-seq). We exposed the typical intertidal seagrass Zostera japonica to 0 and 50 μM of copper (Cu), lead (Pb), and cadmium (Cd) under laboratory conditions. A total of 18,266 differentially expressed genes (DEGs) were identified, of which 2001 co-expressed genes directly related by Cu, Pb, and Cd stress. We also examined the effects of short-term heavy metal Cu, Pb, and Cd pulses on the accumulation of metals in Z. japonica and showed metal concentrations were higher in the shoots than in roots. Twelve differentially expressed genes were further analyzed for expression differences using real-time quantitative polymerase chain reaction (RT-qPCR). Our data suggest that as coastal seawater pollution worsens, the sensitive genes identified in this study may be useful biomarkers of sublethal effects and provide fundamental information for Z. japonica resistant gene engineering.

show abstract

Molecular physiology reveals ammonium uptake and related gene expression in the seagrass Zostera muelleri

Cited by 20 publications

References 50 publications

Stress Memory in Seagrasses: First Insight Into the Effects of Thermal Priming and the Role of Epigenetic Modifications

Stress Memory in Seagrasses: First Insight Into the Effects of Thermal Priming and the Role of Epigenetic Modifications

Metabolomics and traditional indicators unveil stress of a seagrass (Cymodocea nodosa) meadow at intermediate distance from a fish farm

Which Genes in a Typical Intertidal Seagrass (Zostera japonica) Indicate Copper-, Lead-, and Cadmium Pollution?

Contact Info

Product

Resources

About