Molecular phylogeography of Culex quinquefasciatus mosquitoes in central Bangladesh

Hasan, Azeem; Suguri, Setsuo; Ahmed, Syed Minhaj Uddin; Fujimoto, Chigusa; Harada, Masakazu; Rahman, Shaikh Mizanur; Zaman, Rashid Uz; Kakehi, Yoshiyuki

doi:10.1016/j.actatropica.2009.07.005

Cited by 14 publications

(8 citation statements)

References 48 publications

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“…pipiens mitochondrial genes has been reported by GUILLEMAUD et al 17 . HASAN et al 18 also found low diversity (Hd = 0.502 and π = 0.0007) in the Cx. quinquefasciatus cox2 of populations from central Bangladesh, potentially a result of a recent common mitochondrial ancestor.…”

Section: Discussionmentioning

confidence: 89%

Low genetic diversity in Wolbachia-Infected Culex quinquefasciatus (Diptera: Culicidae) from Brazil and Argentina

Morais

Almeida

Suesdek

et al. 2012

Rev. Inst. Med. trop. S. Paulo

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SUMMARYCulex quinquefasciatus is a vector of human pathogens, including filarial nematodes and several viruses. Although its epidemiological relevance is known to vary across geographical regions, an understanding of its population genetic structure is still incipient. In light of this, we evaluated the genetic diversity of Cx. quinquefasciatus and Cx. pipiens x Cx. quinquefasciatus hybrids collected from nine localities in Brazil and one site in Argentina. We used mitochondrial genes cox1 and nd4, along with the coxA and wsp genes of the maternally-inherited Wolbachia endosymbiont. The nd4 fragment was invariant between samples, whilst cox1 exhibited four haplotypes that separated two types of Cx. quinquefasciatus, one clustered in southern Brazil. Low sequence diversity was generally observed, being discussed. Both Brazilian and Argentinian mosquitoes were infected with a single Wolbachia strain. As reported in previous studies with these populations, cox1 and nd4 diversity is not congruent with the population structure revealed by nuclear markers or alar morphology. Future Cx. quinquefasciatus research should, if possible, evaluate mtDNA diversity in light of other markers.

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Section: Discussionmentioning

confidence: 89%

Low genetic diversity in Wolbachia-Infected Culex quinquefasciatus (Diptera: Culicidae) from Brazil and Argentina

Morais

Almeida

Suesdek

et al. 2012

Rev. Inst. Med. trop. S. Paulo

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“…Published genomic DNA or mRNA sequences derived from multiple isolates of known genes for Cx. pipiens compex (Arensburger et al 2010, Hasan et al 2009) were blasted against the Cpq supercontig sequences using the Ensembl Blast tool (http://metazoa.ensembl.org/Culex_quinquefasciatus/blastview). The supercontig sequences with the highest identity score to the query gene sequences were selected for sequencing.…”

Section: Methodsmentioning

confidence: 99%

High Degree of Single Nucleotide Polymorphisms in California Culex pipiens (Diptera: Culicidae) sensu lato

Seifert

Nieman

et al. 2012

jnl. med. entom.

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Resolution of systematic relationships among members of the Culex pipiens (L.) complex has important implications for public health as well as for studies on the evolution of sibling species. Currently held views contend that in California considerable genetic introgression occurs between Cx. pipiens and Cx. quinquefasciatus Say, and as such, these taxa behave as if they are a single species. Development of high throughput SNP genotyping tools for the analysis of Cx. pipiens complex population structure is therefore desirable. As a first step toward this goal, we sequenced 12 gene fragments from specimens collected in Marin and Fresno counties. On average, we found a higher single nucleotide polymorphism (SNP) density than any other mosquito species reported thus far. Coding regions contained significantly higher GC content (median 54.7%) than noncoding regions (42.4%; Wilcoxon rank sum test, P = 5.29 × 10−5). Differences in SNP allele frequencies observed between mosquitoes from Marin and Fresno counties indicated significant genetic divergence and suggest that SNP markers will be useful for future detailed population genetic studies of this group. The high density of SNPs highlights the difficulty in identifying species within the complex and may be associated with the large degree of phenotypic variation observed in this group of mosquitoes.

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“…Often more than one marker will be used, with both mitochondrial and nuclear genes exhibiting utility in distinguishing species (Lin and Danforth 2004). In mosquito barcoding studies, a variety of nuclear markers have been used, including elongation factor-1 alpha (EF-1a), acetylcholinesterase 2 (ace-2), alpha amylase, zinc finger, and internal transcribed spacer subunit 2 (ITS2) (Foley et al 2007;Hasan et al 2009;Hemmerter et al 2009;Puslednik et al 2012). Using multiple genes can help to distinguish members of species complexes and subgroups, which are closely related species that may not be genetically distinct when using just one barcoding region (Foster et al 2013;Jiang et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Molecular identification of mosquitoes (Diptera: Culicidae) in southeastern Australia

Batovska

Blacket

Brown

et al. 2016

Ecology and Evolution

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DNA barcoding is a modern species identification technique that can be used to distinguish morphologically similar species, and is particularly useful when using small amounts of starting material from partial specimens or from immature stages. In order to use DNA barcoding in a surveillance program, a database containing mosquito barcode sequences is required. This study obtained Cytochrome Oxidase I (COI) sequences for 113 morphologically identified specimens, representing 29 species, six tribes and 12 genera; 17 of these species have not been previously barcoded. Three of the 29 species ─ Culex palpalis, Macleaya macmillani, and an unknown species originally identified as Tripteroides atripes ─ were initially misidentified as they are difficult to separate morphologically, highlighting the utility of DNA barcoding. While most species grouped separately (reciprocally monophyletic), the Cx. pipiens subgroup could not be genetically separated using COI. The average conspecific and congeneric p‐distance was 0.8% and 7.6%, respectively. In our study, we also demonstrate the utility of DNA barcoding in distinguishing exotics from endemic mosquitoes by identifying a single intercepted Stegomyia aegypti egg at an international airport. The use of DNA barcoding dramatically reduced the identification time required compared with rearing specimens through to adults, thereby demonstrating the value of this technique in biosecurity surveillance. The DNA barcodes produced by this study have been uploaded to the ‘Mosquitoes of Australia–Victoria’ project on the Barcode of Life Database (BOLD), which will serve as a resource for the Victorian Arbovirus Disease Control Program and other national and international mosquito surveillance programs.

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Molecular phylogeography of Culex quinquefasciatus mosquitoes in central Bangladesh

Cited by 14 publications

References 48 publications

Low genetic diversity in Wolbachia-Infected Culex quinquefasciatus (Diptera: Culicidae) from Brazil and Argentina

Low genetic diversity in Wolbachia-Infected Culex quinquefasciatus (Diptera: Culicidae) from Brazil and Argentina

High Degree of Single Nucleotide Polymorphisms in California Culex pipiens (Diptera: Culicidae) sensu lato

Molecular identification of mosquitoes (Diptera: Culicidae) in southeastern Australia

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