Molecular Phylogeny of the Family Ophryscolecidae (Ciliophora, Litostomatea) Inferred from 18S rDNA Sequences

Abstract

The 18S rDNA was used to infer oral ciliature patterns of evolution within the family Ophryoscolecidae, with the addition of five new sequences of ciliates from the genus Ostracodinium. Our data confirmed the monophyly of the subfamilies Entodiniinae and Ophryoscolecinae, but more analysis would be required for the definition of the status of the subfamily Diplodiniinae. The oral infraciliature patterns reflect evolutionary divergence in the family Ophryscolecidae, observing monophyly on Entodinium-type, Diplodinium-type, Ostracodinium-type, Epidinium-type, and Ophryoscolex-type. The ancestral infraciliature of Entodinium-type cannot be proven, however, the position of Entodinium-type showed closer of Diplodinium-type than Ophryoscolex-type, corroborating previous studies using morphological characters. The observed inconsistencies reflect the need to increase the number of 18S rDNA sequences to family Ophryoscolecidae and investigate the evolution of this group using other molecular markers.

Phylogenetic Analyses Support Validity of Genus Eodinium (Ciliophora, Entodiniomorphida, Ophryoscolecidae)

“…Our analyses suggest that other characters, such as the oral infraciliature, as proposed by Rossi et al. (), might be more relevant in the family Ophryoscolecidae.…”

“…; Rossi et al. ). Although some phylogenetic analyses corroborate phylogenetic hypothesis based on morphological data (Wright and Lynn ,b; Wright et al.…”

“…Since phylogenetic studies on the family Ophryoscolecidae are still incipient (Rossi et al. ), the phylogenetic placement and validity of genus Eodinium remains unclear. The present work investigates the phylogenetic relationship of genus Eodinium based on molecular data (18S rDNA) and expands the current knowledge about the systematics of the family Ophryoscolecidae.…”

Rumen Ciliate Biota of Domestic Cattle (Bos taurus taurus) in İstanbul, Turkey and Infraciliature of Metadinium medium (Entodiniomorphida, Ophryoscolecidae)

“…There are thirteen buccal infraciliature patterns with together M. medium-type in Ophryoscolecidae and phylogenetic relationships between six of them, Entodini um-type, Diplodinium-type, O. gracile-type, O. mam mosum-type, Epidinium-type, and Ophryoscolex-type were also confirmed with the molecular study (Rossi et al 2015).…”

Advances In The Understanding of The Commensal Eukaryota And Viruses Of The Herbivore Gut

“…The first molecular phylogenies (Wright and Lynn, 1997a,b,c;Wright et al, 1997) corroborated the initial morphological studies placing trichostomatids as a monophyletic group within the Litostomatea. Starting from early 2000s and with the increasing availability of 18S rRNA gene sequences of members of the subclass Trichostomatia in public repositories (Cameron et al, 2001a(Cameron et al, , 2003Cameron and O'Donoghue, 2004b;Strüder-Kypke et al, 2007;Ito et al, 2010Ito et al, , 2014Pomajbíková et al, 2010Pomajbíková et al, , 2013Snelling et al, 2011;Chistyakova et al, 2014;Moon-Van der Staay et al, 2014;Grim et al, 2015;Rossi et al, 2015;Bardele et al, 2017;Cedrola et al, , 2019, the internal phylogenetic relationships within the subclass began to be elucidated. This caused a revolution in their systematics and revealed several taxonomic incongruences, mainly with respect to Entodiniomorphida and Vestibuliferida, for which the grouping based on morphological features does not seem to hold.…”