Molecular phylogeny of Lathyrus species: insights from sequence-related amplified polymorphism markers

Marghali, Sonia; Touati, Asma; Gharbi, M.; Sdouga, Dorra; Trifi-Farah, Neïla

doi:10.4238/gmr.15017198

Cited by 8 publications

(4 citation statements)

References 33 publications

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“…The molecular markers can be used to identify the positions, number, effect of genes or quantitative trait loci that controls pest, disease, protein, β-ODAP concentration, and other traits (Campbell et al 1993). For this purpose, RAPD, RFLP, SRAP, AFLP (Marghali et al 2016) and the microsatellite or simple sequence repeat markers are appropriate (Soren et al 2015). The expressed sequence tags available in public domain databases are very limited for Lathyrus.…”

Section: Genetic Research In Lathyrus Sativusmentioning

confidence: 99%

Grass pea (Lathyrus sativus) a Crop of Future for Sustainable Agriculture in The Changing Environmental Conditions

P.S¹

2023

IJMRA

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Grass pea (Lathyrus sativus) is an ideal pulse crop for sustainable agriculture due to its superior agronomic traits such as deep penetrating root system, resistance to many biotic and abiotic stresses and rich protein content. Even with these superior agronomic traits, the crop is considered as an orphan due to its lower productivity and presence of neurotoxin, b-N-ozalyl-L-adiamino-propanoic acid (ODAP). By following classical and mutational breeding approaches scientists have developed varieties with lower ODAP (< 0.06 %) and efforts are underway to reduce it to zero. However, till date, there are limited biotechnological research in Lathyrus sativus for targeted crop improvement due to unavailability of genome sequence. The recently published draft genome sequence of European accession (LS007) is an important breakthrough and this would help in developing varieties with lower neuro toxin and higher productivity. In this review, the superior agronomic traits, resilience of the crops to various biotic and abiotic stresses, latest genetic research and biotechnological tools developed in L. sativus, are discussed.

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Section: Genetic Research In Lathyrus Sativusmentioning

confidence: 99%

Grass pea (Lathyrus sativus) a Crop of Future for Sustainable Agriculture in The Changing Environmental Conditions

P.S¹

2023

IJMRA

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“…Genetic diversity in the Lathyrus genus can be detected by means of diverse molecular markers, including, among others, restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and sequencerelated amplified polymorphism (SRAP) (Chtourou- Ghorbel et al 2001;Marghali et al 2016;Nosrati et al 2012;Tavoletti and Iommarini 2007). Another widely used class of molecular markers are simple sequence repeat (SSR) or microsatellite markers that can be obtained by searching in the expressed sequence tags (ESTs) available at the National Center for Biotechnology Information (NCBI) GenBank for Lathyrus (http://www.ncbi.nlm.nih.gov/genba nk/dbest /dbest _summa ry/) (Lioi et al 2011;Ponnaiah et al 2011;Shiferaw et al 2012;Sun et al 2012;Lioi and Galasso 2013;Soren et al 2015;Gupta et al 2018).…”

Section: Molecular Toolbox For Variety Ameliorationmentioning

confidence: 99%

Grass pea (Lathyrus sativus L.): orphan crop, nutraceutical or just plain food?

Lambein

Travella

Kuo

et al. 2019

Planta

124

113

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Main conclusion Although grass pea is an environmentally successful robust legume with major traits of interest for food and nutrition security, the genetic potential of this orphan crop has long been neglected. Grass pea (Lathyrus sativus L.) is a Neolithic plant that has survived millennia of cultivation and has spread over three continents. It is a robust legume crop that is considered one of the most resilient to climate changes and to be survival food during drought-triggered famines. The hardy penetrating root system allows the cultivation of grass pea in various soil types, including marginal ones. As an efficient nitrogen fixer, it meets its own nitrogen requirements and positively benefits subsequent crops. However, already in ancient India and Greece, overconsumption of the seeds and a crippling neurological disorder, later coined neurolathyrism, had been linked. Overemphasis of their suspected toxic properties has led to disregard the plant's exceptionally positive agronomic properties and dietary advantages. In normal socioeconomic and environmental situations, in which grass pea is part of a balanced diet, neurolathyrism is virtually non-existent. The etiology of neurolathyrism has been oversimplified and the deficiency in methionine in the diet has been overlooked. In view of the global climate change, this very adaptable and nutritious orphan crop deserves more attention. Grass pea can become a wonder crop if the double stigma on its reputation as a toxic plant and as food of the poor can be disregarded. Additionally, recent research has exposed the potential of grass pea as a health-promoting nutraceutical. Development of varieties with an improved balance in essential amino acids and diet may be relevant to enhance the nutritional value without jeopardizing the multiple stress tolerance of this promising crop.

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“…Low ODAP (0.04-0.1%) BARI Khesari-1, BARI Khesari-2, BARI Khesari-3, BARI Khesari-4, BARI Khesari-5 BINA Khesari-1 Bangladesh Malek et al, 1996;Kumar et al, 2013 Ratan, Prateek, P-24, Mahateora, Nirmal, Bidhan Khesari-1 India Lal et al, 1985;Pandey et al, 1996 for measuring variability among the Ethiopian grass pea resulted in non-significant correlation with morphological diversity (Tadesse and Bekele, 2001). In grass pea breeding programs, diverse molecular markers like random amplified polymorphic DNA (RAPD); restriction fragment length polymorphism (RFLP); amplified fragment length polymorphism (AFLP); inter simple sequence repeat (ISSR); expressed sequence tag-simple sequence repeats (EST-SSR); sequence-related amplification polymorphism (SRAP); and sequence tagged site (STS) have been utilized for deciphering diversity and phylogenetic relationship among the species (Hanada and Hirai, 2000;Chtourou-Ghorbel et al, 2001;Tavoletti and Iommarini, 2007;Lioi et al, 2011;Shiferaw et al, 2012;Soren et al, 2015;Marghali et al, 2016;Gupta et al, 2018).…”

Section: Traitmentioning

confidence: 99%

Current Perspectives on Reducing the β-ODAP Content and Improving Potential Agronomic Traits in Grass Pea (Lathyrus sativus L.)

Das

Parihar

Barpete³

et al. 2021

Front. Plant Sci.

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Grass pea is well-established as one of the most resilient and versatile crops that can thrive under extreme climatic circumstances such as cold, heat, drought, salt-affected soils, submergence, and excessive rainfall along with resistance to several diseases and pests. However, despite the awareness of its virtues, its cultivation globally has decreased recently owing to the presence of a neurotoxin, β-N-oxalyl-L-α, β-diaminopropionic acid (β-ODAP), in the seedlings and seeds of this legume, which has been reported to cause neurolathyrism, a non-reversible neurological disorder in humans and animals. Significant repositories of Lathyrus germplasm are available across countries that have provided access to a wide range of agro-morphological traits as well as the low β ODAP content. Efforts have been made worldwide to use these germplasms for the genetic enhancement of grass pea to make this food safe for human consumption. Efforts on molecular breeding of this crop are also lagging. However, during the last decade, the research scenario has changed with some efforts being made toward improving this climate resilient pulse in terms of genomic resources. Molecular markers have also been used to evaluate the interspecific diversity as well as the phylogenetic relationship among the species and mapping studies. Intron-targeted amplified polymorphic, genomic simple sequence repeat, resistance genes analogs, and disease resistance markers developed for other legume species have been successfully cross-amplified in grass pea. Transcriptomic studies have recently been undertaken on grass pea by deploying several second-generation sequencing techniques. In addition, a few studies have attempted to unveil the genes and the underlying mechanism conferring biotic and abiotic stress or regulating the pathway of β-ODAP in grass pea. Proteomics has accelerated the identification studies on differential proteomes in response to salinity and low-temperature stress conditions for unveiling the common signaling pathways involved in mitigating these abiotic stresses and in discovering differentially regulated proteins. In grass pea, a metabolomics approach has been used to identify the metabolic processes associated with β-ODAP synthesis. Genome sequencing of grass pea is under way which is expected to be vital for whole-genome re-sequencing and gene annotation toward the identification of genes with novel functions. Recently, a draft genome sequence of grass pea was developed, and some efforts are underway to re-sequence a diverse panel of grass pea comprising 384 germplasm lines. Owing to the scantiness of a successful transformation protocol, research on the application of modern approaches of genome editing like the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) or CRISPR-associated protein 9 (CRISPR/Cas9) system for the engineering of signaling pathways or regulatory mechanisms seeks immediate attention to reduce the β-ODAP content in seeds and to improve the potential agronomic traits in grass pea.

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Molecular phylogeny of Lathyrus species: insights from sequence-related amplified polymorphism markers

Cited by 8 publications

References 33 publications

Grass pea (Lathyrus sativus) a Crop of Future for Sustainable Agriculture in The Changing Environmental Conditions

Grass pea (Lathyrus sativus) a Crop of Future for Sustainable Agriculture in The Changing Environmental Conditions

Grass pea (Lathyrus sativus L.): orphan crop, nutraceutical or just plain food?

Current Perspectives on Reducing the β-ODAP Content and Improving Potential Agronomic Traits in Grass Pea (Lathyrus sativus L.)

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