Molecular phylogeny of a genetically divergent hantavirus harbored by the Geoffroy's rousette ( Rousettus amplexicaudatus ), a frugivorous bat species in the Philippines

Arai, Satoru; Taniguchi, Satoshi; Aoki, Keita; Yoshikawa, Yasuhiro; Kyuwa, Shigeru; Tanaka‐Taya, Keiko; Masangkay, Joseph S.; Omatsu, Tsutomu; Puentespina, Roberto; Watanabe, Shumpei; Alviola, Phillip A.; Alvarez, James; Eres, Eduardo; Cosico, Edison; Quibod, Ma. Niña Regina M.; Morikawa, Shigeru; Yanagihara, Richard; Oishi, Kazunori

doi:10.1016/j.meegid.2016.08.008

Cited by 19 publications

(36 citation statements)

References 29 publications

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“…In short, we have shown that hantaviruses are infecting bats of different trophic groups (Table ), which is distinct from previous research on Old World bats that found only insectivorous and frugivorous bats to be infected with non‐pathogenic hantaviruses in the genera Loanvirus , and Mobatvirus within the family Hantaviridae (Arai et al, ; Gu et al, ; Guo et al, ; Laenen et al, ; Xu et al, ). Similar studies in southeastern Brazil have shown only phyllostomid bats infected with hantavirus (Araujo et al, ; Sabino‐Santos et al, ).…”

Section: Resultscontrasting

confidence: 79%

Hantavirus antibodies among phyllostomid bats from the arc of deforestation in Southern Amazonia, Brazil

Sabino‐Santos

Ferreira

Silva

et al. 2019

Transbound Emerg Dis

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In order to determine whether southern Amazonian bats could harbour hantaviruses we, serologically and molecularly, screened blood, saliva, excreta and organ tissues of 47 bats captured from September to December 2015. We found that only phyllostomid bats presented antibodies against hantavirus. The seropositive bats belonged to two species of Phyllostomid bats: the greater spear-nosed bat Phyllostomus hastatus (omnivorous) and the gnome fruit-eating bat Dermanura gnoma. The overall seroprevalence was of 4.2%. Therefore, we show here that hantaviruses are circulating among phyllostomid bats in the Amazonian arc of deforestation. K E Y W O R D S arc of deforestation, hantavirus infection, Neotropical bats, spillover S U PP O RTI N G I N FO R M ATI O N Additional supporting information may be found online in the Supporting Information section.

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Section: Resultscontrasting

confidence: 79%

Hantavirus antibodies among phyllostomid bats from the arc of deforestation in Southern Amazonia, Brazil

Sabino‐Santos

Ferreira

Silva

et al. 2019

Transbound Emerg Dis

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“…Apart from the four complete genomic sequences reported here (Table 2), the sequences of representative bat-borne HVs published to date in GenBank show that many are not of complete genomes or even of a full-length gene segment [5–7, 11]. Currently Laibin, Longquan and Quezon viruses are the only bat-borne HVs approved by ICTV so far [6, 9, 12]. Among completed genomes, there are the complete coding sequences of the three gene segments of BRNV from the Czech Republic [13].…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence, cross antigenicity and circulation sphere of bat-borne hantaviruses revealed by serological and antigenic analyses

et al. 2019

PLoS Pathog

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Bats are newly identified reservoirs of hantaviruses (HVs) among which very divergent HVs have been discovered in recent years. However, their significance for public health remains unclear since their seroprevalence as well as antigenic relationship with human-infecting HVs have not been investigated. In the present study archived tissues of 1,419 bats of 22 species from 6 families collected in 5 south and southwest provinces in China were screened by pan-HV RT-PCR following viral metagenomic analysis. As a result nine HVs have been identified in two bat species in two provinces and phylogenetically classified into two species, Laibin virus (LAIV, ICTV approved species, 1 strain) and Xuan son virus (XSV, proposed species, 8 strains). Additionally, 709 serum samples of these bats were also analyzed by ELISA to investigate the seroprevalence and cross-reactivity between different HVs using expressed recombinant nucleocapsid proteins (rNPs) of LAIV, XSV and Seoul virus (SEOV). The cross-reactivity of some bat sera were further confirmed by western blot (WB) using three rNPs followed by fluorescent antibody virus neutralization test (FAVNT) against live SEOV. Results showed that the total HV seropositive rate of bat sera was 18.5% (131/709) with many cross reacting with two or all three rNPs and several able to neutralize SEOV. WB analysis using the three rNPs and their specific hyperimmune sera demonstrated cross-reactivity between XSV/SEOV and LAIV/XSV, but not LAIV/SEOV, indicating that XSV is antigenically closer to human-infecting HVs. In addition a study of the distribution of the viruses identified an area covering the region between Chinese Guangxi and North Vietnam, in which XSV and LAIV circulate within different bat colonies with a high seroprevalence. A circulation sphere of bat-borne HVs has therefore been proposed.

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“…Firstand second-round PCR was performed in 20-µL reaction mixtures, containing 250 µM dNTP, 2 mM MgCl 2 , and 0.25 µM of each primer. LA Taq hot start version (Takara Bio) and AmpliTaq gold 360 DNA polymerase (Applied Biosystems, Foster City, CA, USA) were used at 1 U each for the firstand second-round PCR, respectively (Arai et al, 2016b). Initial denaturation at 94 • C for 2 min was followed by two cycles each of denaturation at 94 • C for 30 s, two-degree step-down annealing from 48 to 38 • C for 40 s, and elongation at 68 • C for 1 min, then 32 cycles of denaturation at 94 • C for 40 s, annealing at 42 • C for 40 s, and elongation at 68 • C for 1 min, in a Veriti thermal cycler (Applied Biosystems) and Mastercycler X50 (Eppendorf, Hamburg, Germany) (Arai et al, 2008(Arai et al, , 2012.…”

Section: Rt-pcr and Dna Sequencingmentioning

confidence: 99%

Genetic Diversity and Phylogeography of Thottapalayam thottimvirus (Hantaviridae) in Asian House Shrew (Suncus murinus) in Eurasia

Kikuchi

Aoki

Ohdachi

et al. 2020

Front. Cell. Infect. Microbiol.

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Murid and cricetid rodents were previously believed to be the principal reservoir hosts of hantaviruses. Recently, however, multiple newfound hantaviruses have been discovered in shrews, moles, and bats, suggesting a complex evolutionary history. Little is known about the genetic diversity and geographic distribution of the prototype shrew-borne hantavirus, Thottapalayam thottimvirus (TPMV), carried by the Asian house shrew (Suncus murinus), which is widespread in Asia, Africa, and the Middle East. Comparison of TPMV genomic sequences from two Asian house shrews captured in Myanmar and Pakistan with TPMV strains in GenBank revealed that the Myanmar TPMV strain (H2763) was closely related to the prototype TPMV strain (VRC66412) from India. In the L-segment tree, on the other hand, the Pakistan TPMV strain (PK3629) appeared to be the most divergent, followed by TPMV strains from Nepal, then the Indian-Myanmar strains, and finally TPMV strains from China. The Myanmar strain of TPMV showed sequence similarity of 79.3-96.1% at the nucleotide level, but the deduced amino acid sequences showed a high degree of conservation of more than 94% with TPMV strains

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Molecular phylogeny of a genetically divergent hantavirus harbored by the Geoffroy's rousette ( Rousettus amplexicaudatus ), a frugivorous bat species in the Philippines

Cited by 19 publications

References 29 publications

Hantavirus antibodies among phyllostomid bats from the arc of deforestation in Southern Amazonia, Brazil

Hantavirus antibodies among phyllostomid bats from the arc of deforestation in Southern Amazonia, Brazil

Seroprevalence, cross antigenicity and circulation sphere of bat-borne hantaviruses revealed by serological and antigenic analyses

Genetic Diversity and Phylogeography of Thottapalayam thottimvirus (Hantaviridae) in Asian House Shrew (Suncus murinus) in Eurasia

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