Molecular phylogeny and morphology reveal cryptic species in Blackwellomyces and Cordyceps (Cordycipitaceae) from Thailand

Mongkolsamrit, Suchada; Noisripoom, Wasana; Tasanathai, Kanoksri; Khonsanit, Artit; Thanakitpipattana, Donnaya; Himaman, Winanda; Kobmoo, Noppol; Luangsa‐ard, J. Jennifer

doi:10.1007/s11557-020-01615-2

Cited by 28 publications

(41 citation statements)

References 43 publications

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“…The second hyperparasite we found associated with O. camponoti-oridani is not only a new species, but a new genus that is sister of Blackwellomyces (Cordycipitaceae), a lineage that produces vivid orange to red stromata, strictly associated with larvae of Coleoptera and Lepidoptera (Mongkolsamrit et al 2020). The BS support is not very strong (BS = 55), which could be explained by the limited available taxa for this part of the phylogenetic tree.…”

Section: Phylogeneticsmentioning

confidence: 79%

Masters of the Manipulator? A Comparative Genomics Study of Niveomyces gen. nov. and Two New Species of Mycoparasites Associated with Ophiocordyceps Camponoti-Floridani

Lebert

Araújo

Linehan

et al. 2021

Preprint

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Fungal hyperparasites can impact ecosystem composition and disease dynamics by modulating their parasite hosts’ population size and transmission rate. Despite their perceived ecosystem impacts and potential to be applied in disease control efforts, hyperparasites are vastly understudied. In this integrative study we formally describe a new genus and two new mycoparasite species that infect the fungus Ophiocordyceps camponoti-floridani, which manipulates the behavior of Florida carpenter ants. Phylogenetic analyses demonstrate that both fungal hyperparasites are distinct species within the families Cordycipitaceae and Ophiocordycipitaceae. The unique morphology, ecology and phylogenetic placement of the Cordycipitaceae species supports its placement in a new genus, Niveomyces. Our field data alludes that both new species, Niveomyces ophiocordycipitis and Polycephalomyces oviedoensis, negatively impact O. camponoti-floridani survival and transmission. Moreover, their macromorphology, along with our field data, suggest that these hyperparasites live an exclusively mycoparasitic lifestyle and do not infect, nor decompose O. camponoti-floridani’s ant host. To find genome signatures that would further confirm their mycoparasitism, we sequenced and annotated both species’ genomes. We then compared the N. ophiocordycipitis and P. oviedoensis genomes to those of other mycoparasites, animal parasites, plant parasites, and saprophytes within the order Hypocreales and searched for previously reported mycoparasite genome signatures. However, our analyses indicate that these signatures are not greatly informative when expanding the number of genomes and lifestyles beyond those of previous studies, using the genome data that is currently available. This highlights the need for additional mycoparasite genomes within the order Hypocreales, as well as data on more closely related animal parasites. As such, this work contributes a starting point for more studies into fungal interactions between mycoparasites and entomopathogens, which have the potential to contribute important knowledge towards efforts to battle the global rise of plant and animal mycoses.

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Section: Phylogeneticsmentioning

confidence: 79%

Masters of the Manipulator? A Comparative Genomics Study of Niveomyces gen. nov. and Two New Species of Mycoparasites Associated with Ophiocordyceps Camponoti-Floridani

Lebert

Araújo

Linehan

et al. 2021

Preprint

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“…Lasergene software (version 6.0, DNASTAR) was applied for the assembling and editing of DNA sequence. The ITS , RPB1 , RPB2 and TEF sequences were downloaded from GenBank, based on Mongkolsamrit et al (2018 , 2020 ) and others selected on the basis of BLAST algorithm-based searches in GenBank (Table 1 ). The multiple datasets of ITS , RPB1 , RPB2 and TEF were aligned and edited by MAFFT v7.037b ( Katoh and Standley 2013 ) and MEGA6 ( Tamura et al 2013 ).…”

Section: Methodsmentioning

confidence: 99%

Multi-gene phylogenetic evidence indicates that Pleurodesmospora belongs in Cordycipitaceae (Hypocreales, Hypocreomycetidae) and Pleurodesmospora lepidopterorum sp. nov. on pupa from China

Chen¹,

Han²,

Liang³

et al. 2021

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A new species, Pleurodesmospora lepidopterorum, isolated from a pupa, is introduced. Morphological comparisons and phylogenetic analyses based on multigene datasets (ITS+RPB1+RPB2+TEF) support the establishment of the new species. Pleurodesmospora lepidopterorum is distinguished from P. coccorum by its longer conidiogenous pegs located in the terminal or lateral conidiophores, and smaller subglobose or ellipsoidal conidia. A combined dataset of RPB1, RPB2, and TEF confirmed the taxonomic placement of Pleurodesmospora in Cordycipitaceae for the first time.

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“…Tropical and sub-tropical regions show the highest known species diversity, particularly in east and south-east Asia [ 24 ]. The genus Cordyceps does not have distinctive unique characteristics that discriminate it from closely related genera such as Blackwellomyces and Samsoniella [ 25 ]. Cordyceps species are parasitic on insects of various orders including Coleoptera, Diptera, Hemiptera, Hymenoptera, Lepidoptera, Orthoptera and also on spider [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

“…The genus Cordyceps does not have distinctive unique characteristics that discriminate it from closely related genera such as Blackwellomyces and Samsoniella [ 25 ]. Cordyceps species are parasitic on insects of various orders including Coleoptera, Diptera, Hemiptera, Hymenoptera, Lepidoptera, Orthoptera and also on spider [ 25 ]. Therefore, their identification relies mainly on molecular tools.…”

Section: Introductionmentioning

confidence: 99%

“…In this study, we generated sequences from PacBio Sequel I of the ITS region combined with D1–D2 LSU domains from nine Cordyceps species including C. blackwelliae, C. cateniannulata, C. chiangdaoensis, C. javanica, C. kuiburiensis, C. lepidopterorum , C. morakotii , C. cf. ninchukispora , and C. tenuipes based on specimens already identified with accuracy from previous studies [ 25 , 26 , 27 , 28 ]. The data allowed us to evaluate the level of intragenomic variation via analyses of sequence divergence and haplotype network.…”

Section: Introductionmentioning

confidence: 99%

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Using High-Throughput Amplicon Sequencing to Evaluate Intragenomic Variation and Accuracy in Species Identification of Cordyceps Species

Paloi

Mhuantong

Luangsa‐ard

et al. 2021

JoF

Self Cite

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While recent sequencing technologies (third generation sequencing) can successfully sequence all copies of nuclear ribosomal DNA (rDNA) markers present within a genome and offer insights into the intragenomic variation of these markers, high intragenomic variation can be a source of confusion for high-throughput species identification using such technologies. High-throughput (HT) amplicon sequencing via PacBio SEQUEL I was used to evaluate the intragenomic variation of the ITS region and D1–D2 LSU domains in nine Cordyceps species, and the accuracy of such technology to identify these species based on molecular phylogenies was also assessed. PacBio sequences within strains showed variable level of intragenomic variation among the studied Cordyceps species with C. blackwelliae showing greater variation than the others. Some variants from a mix of species clustered together outside their respective species of origin, indicative of intragenomic variation that escaped concerted evolution shared between species. Proper selection of consensus sequences from HT amplicon sequencing is a challenge for interpretation of correct species identification. PacBio consensus sequences with the highest number of reads represent the major variants within a genome and gave the best results in terms of species identification.

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Molecular phylogeny and morphology reveal cryptic species in Blackwellomyces and Cordyceps (Cordycipitaceae) from Thailand

Cited by 28 publications

References 43 publications

Masters of the Manipulator? A Comparative Genomics Study of Niveomyces gen. nov. and Two New Species of Mycoparasites Associated with Ophiocordyceps Camponoti-Floridani

Masters of the Manipulator? A Comparative Genomics Study of Niveomyces gen. nov. and Two New Species of Mycoparasites Associated with Ophiocordyceps Camponoti-Floridani

Multi-gene phylogenetic evidence indicates that Pleurodesmospora belongs in Cordycipitaceae (Hypocreales, Hypocreomycetidae) and Pleurodesmospora lepidopterorum sp. nov. on pupa from China

Using High-Throughput Amplicon Sequencing to Evaluate Intragenomic Variation and Accuracy in Species Identification of Cordyceps Species

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