Molecular phylogeny and genetic variability of the Potato virus Y (PVY) strains on the CP-encoding region

Abstract

The Potato virus Y (PVY) is one of the most damaging viruses in cultivated potatoes and other Solanaceous crops. In this work, we studied the molecular phylogeny and the genetic variability of 107 PVY isolates selected from "GeneBank" which classification and country origins have been mentioned. The general phylogenetic profile reveals six main genetic clusters of PVY. However, the tree did not highlight a distribution of the PVY according to his geographic isolation. The genetic divergence within and between the deduced groups and subgroups shows that the minimum inter-group genetic divergence is 11%. The alignments of the deduced amino-acids sequence define consensus for each strain group as well as specific differences between them. The specific amino-acids residues of each strain group were highlighted.

Biological characterization of French Potato virus Y (PVY) isolates collected from PVY‐susceptible or ‐resistant tobacco plants possessing the recessive resistance gene va

“…According to the comparison of nucleotide and amino acid identity of CP sequences, PVY isolates collected in Chinese tobacco fields were classified in three groups, together with reference PVY N , PVY NTN , or PVY O /PVY N ‐W potato isolates (Li et al , 2006). Phylogenetic analyses performed using either CP or full length genome sequences of PVY isolates collected on different solanaceous hosts (Blanco‐Urgoiti et al ., 1998; Lorenzen et al , 2006; Feki & Bouslama, 2008; Moury, 2010) clusters potato PVY isolates in three groups named O, N and C2, corresponding to PVY O , PVY N and PVY C isolates, whereas non‐potato isolates, including isolates collected on tobacco and pepper, constitute a fourth group, C1. Nevertheless, the scientific literature on the tobacco/PVY pathosystem is mainly focused on the interaction between tobacco strain PVY isolates and hosts (Blancard et al ., 1995; Verrier & Doroszewska, 2004).…”