Molecular phylogenetics of Chelonopsis (Lamiaceae: Gomphostemmateae) as inferred from nuclear and plastid DNA and morphology

Abstract

The recently established tribe Gomphostemmateae (Lamiaceae) consists of Gomphostemma, Bostrychanthera and Chelonopsis, but relationships within and between these genera have not been well studied. Only six of the approximately 46 species of Gomphostemmateae have been included in any previous phylogenetic analysis. In the present study, we used DNA sequences of two nuclear regions (ITS, ETS) and five plastid loci (psbA–trnH, rps16, trnL intron, trnL–trnF spacer, trnS–trnG) to reconstruct the phylogeny of the East Asian genus Chelonopsis for the first time. The molecular results indicate that the tribe Gomphostemmateae is monophyletic; Bostrychanthera is embedded in Chelonopsis; and Chelonopsis comprises two clades, one encompassing the taxa of Chelonopsis subg. Chelonopsis and the genus Bostrychanthera and the other consisting of Chelonopsis subg. Aequidens. This split is supported by several morphological characters. These results, which are further strengthened by morphological and cytological data, indicate that Bostrychanthera should be transferred to Chelonopsis. In addition, our results show that within C. subg. Aequidens, sect. Aequidens and sect. Microphyllum are monophyletic. Furthermore, two major clades are concordant with the Sino–Japanese and Sino–Himalayan distribution patterns.

Phylogenetic placement of the enigmatic genus Holocheila (Lamiaceae) inferred from plastid DNA sequences

“…with Chelonopsis Miq. following Xiang & al., 2013). The outgroups included four species of subfamily Scutellarioideae (Holmskioldia sanguinea Retz., Scutellaria hirta Sibth.…”

“…The three sequence regions of Holocheila longipedunculata 1-3, matK and the trnLtrnF region of Cymaria dichotoma 1, and the trnLtrnF region of Hymenopyramis cana and Petraeo vitex multiflora, were newly sequenced. The other sequences were obtained from GenBank published by prior researchers (Lindqvist & Albert, 2002;Scheen & al., 2008Scheen & al., , 2010Scheen & Albert, 2009;Bendiksby & al., 2011;Mathiesen & al., 2011;Xiang & al., 2013).…”

A large-scale chloroplast phylogeny of the Lamiaceae sheds new light on its subfamilial classification

“… 31 ; Gomphostemmateae—Xiang et al . 53 ) have led to changes in the composition of some genera, acceptance of genera not recognized by Harley et al . 16 , and elimination of some genera that were recognized by Harley et al .…”

New combinations and new synonyms in Lamiaceae from China

“…济价值 (Drew & Sytsma, 2012)。该科植物全球广泛 分布, 共236属7,200余种 (Harley et al, 2004), 我国 有该科植物96属970余种 (Li & Hedge, 1994), 为唇 形科的6个多样性中心之一 (Harley et al, 2004)。 近年 来, 我们对国内唇形科和一些重要类群进行了分类 修订及系统学研究, 并随之作了相应的命名处理 Xiang et al, , 2009Xiang et al, , 2010Xiang et al, , 2013Xiang et al, , 2014 …”