Molecular Phylogenetic Studies on the Diatrypaceae Based on rDNA-ITS Sequences

Abstract

The order Diatrypales (Ascomycota) contains one single family, the Diatrypaceae. To obtain insight in the phylogenetic relationships within this family, the complete sequences of the ITS region (ITS1, 5.8S rRNA gene and ITS2) of 53 isolates from the five main genera in the family (Diatrype, Diatrypella, Cryptosphaeria, Eutypa and Eutypella) were determined and aligned for phylogenetic reconstruction. Sequence analysis revealed the presence of tandem repeated motifs 11 nucleotides-long, placed in homologous positions along the ITS1 region. Parsimony analysis established the existence of nine monophyletic groups and one branch with a single isolate of Eutypella quaternata. The phylogenetic relationships established by parsimony analysis did not correlate well with classical taxonomic schemes. None of the five genera included in this study was found to be monophyletic. The genera Diatrype, Eutypa and Cryptosphaeria each were divided into two groups. Isolates of Diatrype flavovirens appeared in a clade separated from the one that grouped Diatrype disciformis and the rest of Diatrype species. The Eutypa strains appeared distributed into two clades, one grouping Eutypa lata and related species (Eutypa armeniacae, Eutypa laevata, Eutypa petrakii), and anoth

Novel taxa of Diatrypaceae from Para rubber (Hevea brasiliensis) in northern Thailand; introducing a novel genus Allocryptovalsa

“…Nitschke 1867 Note -Diatrypella is feature characterized by "ascostromata conical or truncate, cushion-like or discoid, usually delimited by a black zone on host tissues, perithecial ascomata, umbilicate or sulcate ostioles; asci polysporous, cylindrical, with long stalks and ascospores are ovoid to allantoid, hyaline or yellowish" (Vasilyeva & Stephenson 2005, Mehrabi et al 2015, Shang et al 2017. Acero et al (2004) confirmed the polyphyletic groups of Diatrypella in Diatrypaceae based on phylogenetic analysis of ITS rDNA data and this was concurred from previous studies (de Almeida et al 2016, Mehrabi et al 2016, Shang et al 2017. 1 -Phylogram generated from the best scoring of the RAxML tree based on a combined ITS and β-tubulin sequence data.…”

“…Taxa in Diatrypaceae are difficult to distinguish based on the morphological characters (Acero et al 2004, Vasilyeva & Stephenson 2005. Therefore, multigene phylogenetic investigations are needed to clarify phylogenetic affinities of the genera in this family.…”

“…Species of this family are mostly saprobes inhibiting wood and bark of various angiosperms and some species (e.g. Eutypa leptoplaca, Eutypella lata, E. microtheca, Cryptosphaeria pullmanensis, Cryptovalsa ampelina, and Diatrypella vulgaris) have been reported as pathogens and/or endophytes (Acero et al 2004, Trouillas et al 2004, 2010, 2011, Trouillas & Gubler 2010, 2016, Grassi et al 2014, PaolinelliAlfonso et al 2015, Mehrabi et al 2016, Shang et al 2017.…”

Taxonomy and DNA phylogeny of Diatrypaceae associated with Vitis vinifera and other woody plants in Australia

“…Analyses confirm the observation by Acero et al (2004) that the current taxonomic scheme for the Diatrypaceae may not reflect the true evolutionary relationships of these fungi and limits between genera. Moreover, the present analyses did not allow the evolutionary history of Diatrypaceae to be elucidated, as bootstrap values were small at deep nodes within the various tree topologies.…”

“…The current classification of diatrypaceous genera remains provisional and there is an urgent need to revise the classification of the family and test the significance of generic concepts using molecular phylogeny. Preliminary attempts at phylogenetic classification using molecular data as well as morphological characters remained inconclusive regarding the evolutionary relationships of these fungi (Acero et al 2004;Carmarán et al 2006;Trouillas et al 2010a, b). In Australia, little work has been conducted to investigate the diversity and taxonomy of diatrypaceous fungi.…”