Molecular phylogenetic analysis of the Microphalloidea Ward, 1901 (Trematoda: Digenea)

Abstract

Phylogenetic interrelationships of 32 species belonging to 18 genera and four families of the superfamily Microphalloidea were studied using partial sequences of nuclear lsrDNA analysed by Bayesian inference and maximum parsimony. The resulting trees were well resolved at most nodes and demonstrated that the Microphalloidea, as represented by the present data-set, consists of three main clades corresponding to the families Lecithodendriidae, Microphallidae and Pleurogenidae + Prosthogonimidae. Interrelationships of taxa within each clade are considered; as a result of analysis of molecular and morphological data, Floridatrema Kinsella & Deblock, 1994 is synonymised with Maritrema Nicoll, 1907, Candidotrema Dollfus, 1951 with Pleurogenes Looss, 1896, and Schistogonimus Lühe, 1909 with Prosthogonimus Lühe, 1899. The taxonomic value of some morphological features, used traditionally for the differentiation of genera within the Lecithodendriidae and Prosthogonimidae, is reconsidered. Previous systematic schemes are discussed from the viewpoint of present results, and perspectives of future studies are outlined.

DNA sequencing of Digenea nuclear lsrDNA of the whiskered brown bat,Myotis aurescens (Vespertilionidae: Chiroptera), from Turkey

“…Total volume of the PCR mixture was 25 µL. Forward 300F (5ʹ-CAAGTACCGTGAGGGAAAGTTG-3ʹ) and reverse ECD2 (5ʹ-CTTGGTCCGTGTTTCAAGACGGG-3ʹ) primer sequences (Tkach et al, 2003) were used with the following conditions: first denaturation for 10 min at 94 °C, then 38 cycles of denaturation for 30 s at 94 °C, annealing for 30 s at 60 °C, and extension for 30 s at 72 °C, ending with a final extension for 10 min at 72 °C. A Gene Amp PCR System 9700 (in 9600 emulation mode) was used for first PCR and sequencing PCR.…”

The taxonomic identity and phylogenetic relationships of Cercaria pugnax and C. helvetica XII (Digenea: Lecitho-dendriidae) based on morphological and molecular data

“…In this case, forward primer d58f (5'-GCGGTGGATCACTCGGCTCGTG-3') localised in the 5.8 gene and reverse primer digl2r (5'-CCGCTTAGTGATAT-GCTT-3') localised at the 5' end of the 28S gene were used for the PCR amplification and sequencing. The PCR reactions were performed following the protocols described by Tkach et al (2003). Contiguous sequences were assembled using Sequencher 4.7 software (Gene Codes Corporation).…”

Molecular characterization of two opecoelid trematodes from fishes in the Gulf of Mexico, with a description of a new species of Helicometra

“…Sequencing reactions used the previous primers and the internal forward primers digl2 (5'-AAGCATATCACTAAGCGG-3'), 300F (5'-CAAGTACCGTGAGGGAAAGTTG-3'), and 900F (5'-CCGTCTTGAAACACGGACCAAG-3') and internal reverse primer digl2R (5'-CCGCTTAGTGATATGCTT-3'), 300R (5'-CAACTTTCCCTCACGGTACTTG-3'), and ECD2 (5'-CTTGGTCCGTGTTTCAAGACGGG-3'). The PCR reactions were performed under the protocols described by Tkach et al (2003).…”