DNA sequencing of Digenea nuclear lsrDNA of the whiskered brown bat,Myotis aurescens (Vespertilionidae: Chiroptera), from Turkey

Abstract

Six species of Trematoda (Prosthodendrium ascidia, Prosthodendrium longiforme, Lecithodendrium linstowi, Plagiorchis muelleri, Plagiorchis vespertilionis, and Plagiorchis koreanus) of Myotis aurescens from Bursa Province, Turkey, were examined for DNA sequencing of the 28S ribosomal gene region. A partial sequence of the 28S ribosomal gene from digenean species was studied using BLAST and found to be a useful region for helminth species diagnosis.

Morphological and molecular characterization of plagiorchiid trematodes (Plagiorchis: Plagiorchiidae, Digenea) from bats with redescription of Plagiorchis mordovii Shaldybin, 1958

“…Thus, Plagiorchis vespertilionis occurs predominantly in M. daubentonii. It has been reported from other bats (Soltys 1959;Skvortsov 1972;Sharpilo and Iskova 1989;Tkach et al 2000b;Shimalov et al 2011;Kirillov et al 2012aKirillov et al , 2012bKirillov et al , 2015Sumer and Yildirimhan, 2017Lebedeva et al 2020;Shimalov 2021;Kirillova et al 2023), but, in our opinion, these findings require verification. The authors might have dealt with closely related P. muelleri and P. koreanus, which are common in bats.…”

“…Plagiorchis vespertilionis is a common parasite of bats found in many European countries (Odening 1964; Zdzitowiecki 1970; Matskasi 1967, 1973; Skvortsov 1972; Chiriac and Barbu 1973; Bakke and Mehl 1977; Tkach and Sharpilo 1990; Sharpilo and Tkach 1992; Ricci 1995; Sumer and Yildirimhan 2017, 2018, 2019; Shimalov 2021). In Russia, this species is known from bats in the Astrakhan region (Kurochkin and Kurochkina 1962), the Samara region (Demidova and Vekhnik 2004; Kirillov et al 2012a, 2012b, 2017), Mordovia (Schaldybin 1964; Kirillov et al 2015; Ruchin et al 2016; Kirillova et al 2018, 2023), Karelia (Lebedeva et al 2020), and Kamchatka (Orlovskaya and Dokuchaev 2017).…”

“…In our opinion, it is more likely that this species was identified as P. koreanus (Braun 1900; Rysavy 1956; Kochseder 1969; Zdzitowiecki 1970; Skvortsov 1972; Groschaft and Tenora 1973; Meszaros and Mas-Coma 1980; Alvarez et al 1991; Shimalov et al 2011; Naumkin and Sivkova 2019). Plagiorchis muelleri is known from Eptesicus serotinus Schreber, 1774 in Belarus (Shimalov et al 2002; Shimalov 2021), from Myotis aurescens Kuzyakin, 1935 and Myotis daubentonii (Kuhl, 1817) in Turkey (Sumer and Yildirimhan 2017, 2018, 2019), from M. brandtii in the Samara region (Demidova and Vekhnik 2004), and from P. auritus and M. brandtii in Karelia (Lebedeva et al 2020). We found P. muelleri in bats of the Samara region and Mordovia (Kirillova et al 2007, 2023; Kirillov et al 2012a, 2012b; 2015; Ruchin et al 2016).…”

“…from Spain (Esteban et al 1999), in bats from Italy (Lanza 1999), in Myotis spp. from Turkey (Sumer and Yildirimhan 2017, 2018, 2019), and in the United Kingdom from Pipistrellus spp. (Lord et al 2012).…”

Trematodes of Genera Gyrabascus and Parabascus from Bats in European Russia: Morphology and Molecular Phylogeny

“…Tkach et al [ 40 , 41 ] have repeatedly emphasized that morphological features are not always sufficient for the identification of trematode species and genera and that confirmation by molecular data is required. Only a few studies have been conducted on DNA sequencing of bat helminths [ 26 , 30 , 40 , 41 , 42 , 43 , 44 ].…”