Molecular pathways associated with transcriptional alterations in hyperparathyroidism

Abstract

Abstract.Hyperparathyroidism is characterized by the oversecretion of parathyroid hormone biochemically and increased cell proliferation histologically. Primary and secondary hyperparathyroidism exhibit distinct pathophysiology but share certain common microscopic features. The present study performed the first genome-wide expression analysis directly comparing the expression profile of primary and secondary hyperparathyroidism. Microarray gene expression analyses were performed in parathyroid tissues from 2 primary hyperparathyroidism patients and 3 secondary hyperparathyroidism patients. Unsupervised hierarchical clustering analysis identified two natural subgroups containing different types of hyperparathyroidism. Combined with additional data extracted from a publicly available database, a meta-signature was constructed to represent an intersection of two sets of differential expression profile. Multiple pathways were identified that are aberrantly regulated in hyperparathyroidism. In primary hyperparathyroidism, dysregulated pathways included cell adhesion molecules, peroxisome proliferator-activated receptor signaling pathway, and neuroactive ligand-receptor interaction. Pathways implicated in secondary hyperparathyroidism included tryptophan metabolism, tight junctions, renin-angiotensin system, steroid hormone biosynthesis, and O-glycan biosynthesis. The present study demonstrates that different pathophysiology is associated with differential gene profiling in hyperparathyroidism. Several pathways are involved in parathyroid dysregulation and may be future targets for therapeutic intervention.

Transcriptome analysis of papillary thyroid cancer harboring telomerase reverse transcriptase promoter mutation

“…For the hierarchical cluster analysis, the centroid linkage of the Spearman rank correlation was used. Results (colorized matrix) were visualized using the Java TreeView program . We removed genes with low‐level expression, namely genes with a median RSEM value less than the lowest quartile of median RSEM values of all genes .…”

“…Results (colorized matrix) were visualized using the Java TreeView program. 19 We removed genes with low-level expression, namely genes with a median RSEM value less than the lowest quartile of median RSEM values of all genes. 20 Given the skewed distribution of RSEM values of most genes, the RSEM expression levels of each gene were compared between the TERT promoter wild-type and mutant groups using the Mann-Whitney U test.…”

Primary hyperparathyroidism characterized by diffuse homogeneous metastatic pulmonary calcification

“…[ 1 ] It is caused by autonomic oversecretion of parathyroid hormone (PTH) owing to parathyroid adenoma, hyperplasia, or tumor. [ 2 ] It results in hypercalcemia and hypophosphatemia involving multiple parts of the whole body, but mainly the bone and urinary tract systems. As for the lung, PHPTI involvement is relatively rare, [ 3 ] and disperse calcification is the most common symptom in PHPTI.…”

Symptomatic primary hyperparathyroidism in a young woman presenting with multiple skeletal destructions: a case report and review of literature

“…Primary hyperparathyroidism (PHPT) is a rare clinical condition characterized by an autonomic oversecretion of parathyroid hormone caused by a parathyroid adenoma, hyperplasia, or cancer [ 1 , 2 ]. Hypercalcemia and hypophosphatemia have been observed in patients diagnosed with PHPT.…”