Molecular pathogenesis of spontaneous abortions – Whole genome copy number analysis and expression of angiogenic factors

Dimova, Ivanka; Rizov, Marian; Giragosyan, S.; Koprinarova, Miglena; Tzoneva, Д.; Belemezova, Kalina; Hristova-Savova, Mariela; Milachich, Tanya; Djonov, Valentin; Shterev, A

doi:10.1016/j.tjog.2019.11.015

Cited by 5 publications

(6 citation statements)

References 29 publications

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“…Chao et al (34) found that the expression of CXCL12 chemokine and its receptor CXCR4 increased in the abortion material induced in mice with IFN-gamma. In another study, Dimova et al revealed an increased expression of CXCL12 chemokine and its receptor CXCR4 in the decidua of 30 patients with spontaneous abortion compared with 15 patients with elective abortion (35). In the present study, the median protein level and gene expression of CXCL12 and CXCR4 were found to be significantly higher in pregnant women with missed abortion than in pregnant women with voluntary abortion.…”

Section: Discussionsupporting

confidence: 55%

Role of chemokines in early pregnancy loss

et al. 2022

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The present study aimed to compare decidual protein levels and gene expression levels of chemokines between patients with early pregnancy loss and those with voluntary abortion. A total of 15 patients between 6 and 10 gestational weeks, who presented with negative fetal heartbeat to the obstetrics and gynecology outpatient clinics of Gaziosmanpasa Hospital (Yeni Yuzyil University, Istanbul, Turkey) and who had no additional systemic disease and 13 patients between 6 and 10 gestational weeks, who presented with positive fetal heartbeat for voluntary abortion were included in the present study. CX3CL1, CCL17, CXCR4, chemokine ligand 12 (CXCL12) and intercellular adhesion molecule (ICAM)5 protein expression levels were determined by ELISA and gene expression levels by reverse transcription-quantitative PCR in fresh materials recovered after therapeutic curettage. CX3CL1, CCL17, CXCR4, CXCL12 protein levels were significantly higher and ICAM protein level was significantly lower in pregrant women with missed abortion compared with those with voluntary abortion. While the amount of increase in mean CX3CL1, CCL17, CXCR4 and CXCL12 gene expression levels in the tissues of pregnant women with missed abortion was statistically higher than the pregnant women who underwent voluntary abortion, the amount of increase in ICAM5 gene expression was found to be lower (P<0.001) in those with missed abortion. In conclusion, the findings of the present study suggested that CCL17, CX3CL1, CXCL12, CXCR4 and ICAM5 may be associated with missed abortion and may play an important role in placental invasion and the continuation of pregnancy.

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Section: Discussionsupporting

confidence: 55%

Role of chemokines in early pregnancy loss

et al. 2022

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“…SDF-1 участвует в эпителиально-стромальном взаимодействии через рецептор эстрогена-α (ERα) в эндометрии и трофобласте, максимально экспрессируется в зоне имплантации, способствует ремоделированию спиральных артерий и имеет зависимость экспрессии от тканевой гипоксии [35,36].…”

Section: Discussionunclassified

“…Надо сказать, что уже в 1937 году в нашей стране усилиями коллектива ученых из Института мозга был опубликован Атлас большого мозга человека и животных [34], который включал в себя некоторые аннотированные срезы головного мозга плодов человека (рис. ), однако по сегодняшний день последовательно данные о развитии мозга плода человека представлены только в печатных атласах Байер и Альтмана [35][36][37][38]. Эти атласы состоят из общих видов и схем гистологических препаратов серийных срезов мозга плодов на разных сроках внутриутробного развития.…”

Section: атласы развития мозга человека во время внутриутробного онто...unclassified

“…Большая часть современных публикаций, посвященных развитию головного мозга человека, основана на материалах неинвазивных исследований, предоставляющих информацию в основном на макроморфологическом уровне [33]. Материалы гистологических исследований намного подробнее освещают эмбриональный и префетальный периоды развития, сведения о плодном периоде представлены в основном макропрепаратами или схемами [35][36][37][38]53]. Опубликованные данные о развитии мозга человека на разных этапах фетального развития не систематизированы, касаются отдельных аспектов развития или отдельных структур мозга и могут быть противоречивы.…”

Section: атласы развития мозга человека во время внутриутробного онто...unclassified

“…Наиболее распространены типы ВПЧ 16 и 18, указано, что вирусная инфекция может быть важным фактором, способствующим мутации белка p53, причем экспрессия вирусных белков E6 и E7 является показателем биологической активности и онкогенного влияния ВПЧ на патогенез опухолей, и приведены доказательства, что их деятельность может сопровождать все этапы патогенеза РМЖ. Интересны результаты, демонстрирующие отрицательную экспрессию вирусных частиц в биопсиях, а также отсутствие связи между наличием белков E6, E7 в опухоли и ее морфологическим и молекулярным подтипом, гормональным статусом или стадией заболевания [34][35][36][37][38]. Также продемонстрировано, что ВПЧ-инфекция при цервикальной неоплазии сопровождает развитие ассоциированного с этим вирусом РМЖ.…”

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2023

Clin. Exp. Morphology

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Тираж 500 экземпляров Издательство «Группа МДВ» www.mdv-group.ru Подписные индексы: 70045 по каталогу агентства «Урал-Пресс» 70045 по каталогу «Пресса России» через агентство АРЗИ Свидетельство о регистрации средства массовой информации ПИ № ФС77-83521 от 24 июня 2022 года. © НИИ морфологии человека им. акад. А.П. Авцына ФГБНУ РНЦХ им. акад. Б.В. Петровского

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Morphological Features of the Endometrium Transformation in Recurrent Miscarriage

2022

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The problems of pathological diagnosis of recurrent miscarriage are relevant in modern reproductive medicine. One of the important molecular markers of endometrium is the progesterone-induced blocking factor, which is induced by progesterone under the influence of activated lymphocytes and has an immunomodulatory effect on the implantation characteristics of the endometrium. In the process of trophoblast invasion, a special role is also played stromal cell factor molecules synthesized by endometrial and trophoblast cells, as a mechanism that potentiates the susceptibility of the endometrium to the onset and development of pregnancy, trophoblast invasion and embryogenesis in general. The purpose of the study was the histological and immunohistochemical features of the transformation of the endometrium in recurrent miscarriage. It were studied 100 samples of endometrium in case of non-developing pregnancy at a development period of 5-8 weeks, 85 samples in habitual miscarriage and 15 samples in pregnancy interrupted surgically at the request of the woman. The presence of 2 non-developing pregnancies was verified in 57 cases (67,1%), the presence of 3 non-developing pregnancies in 28 cases (32,9%). A histological examination of the abortion material stained with hematoxylin and eosin was performed to verify the morphological transformation of the endometrium. Immunohistochemical study included detection of estrogen and progesterone receptors, stromal cell factor (SDF1), progesterone-induced blocking factor (PIBF). The results of histological and immunohistochemical studies showed that with a complete morphological transformation of the endometrium in the group of patients with habitual miscarriage, there is a violation of the receptor profile. The decrease in PIBF expression in the glands and the stromal component and the expression of SDF1 in the glands of the compact layer of the endometrium is a reflection of the immunological imbalance in the endometrium in recurrent miscarriage. The development of a unified morphological algorithm, taking into account the basic indicators of the processes of transformation of the endometrium with an assessment of its receptor profile and diagnostically significant immunological factors, makes it possible to verify the pathology of the endometrium at the molecular level and justify the need for using pathogenetically substantiated therapy at recurrent miscarriage.

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Molecular pathogenesis of spontaneous abortions – Whole genome copy number analysis and expression of angiogenic factors

Cited by 5 publications

References 29 publications

Role of chemokines in early pregnancy loss

Role of chemokines in early pregnancy loss

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Morphological Features of the Endometrium Transformation in Recurrent Miscarriage

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