Molecular organization of the Drosophila melanogaster Pig-1 gene

Furia, Maria; Digilio, Filomena Anna; Artiaco, Dora; D’Avino, Pier Paolo; Cavaliere, Daniela; Polito, Lino C.

doi:10.1007/bf00360686

Cited by 6 publications

(3 citation statements)

References 16 publications

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“…2). Heterogeneity at the polyadenylation site has also been observed in D. affinidisjunta Adh mRNAs (Rowan and Dickinson 1988) and in Pig-1 gene of D. melanogaster (Furia et al 1991). It seems to be a fairly common phenomenon in Drosophila.…”

Section: Transcriptional Analysis Of Adhmentioning

confidence: 78%

Nucleotide sequence of the genomic region encompassing Adh and Adh-Dup genes of D. lebanonensis (Scaptodrosophila): Gene expression and evolutionary relationships

1994

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The region of the genome of D. lebanonensis that contains the Adh gene and the downstream Adh-dup gene was sequenced. The structure of the two genes is the same as has been described for D. melanogaster. Adh has two promoters and Adh-dup has only one putative promoter. The levels of expression of the two genes in this species are dramatically different. Hybridizing the same Northern blots with a specific probe for Adh-dup, we did not find transcripts for this gene in D. lebanonensis. The level of Adh distal transcript in adults of D. lebanonensis is five times greater than that of D. melanogaster adults. The maximum levels of proximal transcript are attained at different larval stages in the two species, being three times higher in D. melanogaster late-second-instar larvae than in D. lebanonensis first-instar larvae. The level of Adh transcripts allowed us to determine distal and proximal initiation transcription sites, the position of the first intron, the use of two polyadenylation signals, and the heterogeneity of polyadenylation sites. Temporal and spatial expression profiles of the Adh gene of D. lebanonensis show qualitative differences compared with D. melanogaster. Adh and Adh-dup evolve differently as shown by the synonymous and nonsynonymous substitution rates for the coding region of both genes when compared across two species of the melanogaster group, two of the obscura group of the subgenus Sophophora and D. lebanonensis of the victoria group of the subgenus Scaptodrsophila. Synonymous rates for Adh are approximately half those for Adh-dup, while nonsynonymous rates for Adh are generally higher than those for Adh-dup. Adh shows 76.8% identities at the protein level and 70.2% identities at the nucleotide level while Adh-dup shows 83.7% identities at the protein level and 67.5% identities at the nucleotide level. Codon usage for Adh-dup is shown to be less biased than for Adh, which could explain the higher synonymous rates and the generally lower nonsynonymous substitution rates in Adh-dup compared with Adh. Phylogenetic trees reconstructed by distance matrix and parsimony methods show that Sophophora and Scaptodrosophila subgenera diverged shortly after the separation from the Drosophila subgenus.

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Section: Transcriptional Analysis Of Adhmentioning

confidence: 78%

Nucleotide sequence of the genomic region encompassing Adh and Adh-Dup genes of D. lebanonensis (Scaptodrosophila): Gene expression and evolutionary relationships

1994

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“…For Northern analyses, the RNA was fractionated on 1 -2 % agarose formaldehyde gel, transferred to a nylon membrane (Hybond N, Amersham) and then hybridized and washed according to the protocols suggested by Amersham. Sgs-4 mRNA was detected using as a probe the 0-7 kb EcoR I/Hind III genomic fragment containing most of the Sgs-4 coding region (Muskavitch & Hogness, 1982), while to detect Pig-1 and ng-1 transcripts the 7al Pig-1 cDNA clone (Furia et al 1991) and the SCI ng-1 cDNA clone (Furia et al 1990) were used respectively. The genomic fragment aDm2023 (Garfinkel et al 1983) was used as Sgs-3 specific probe.…”

Section: (Iv) Rna Isolation and Northern Analysesmentioning

confidence: 99%

“…It encodes a salivary gland secreted protein (Muskavitch & Hogness, 1980); the same function has been recently suggested for ng-1 (Furia et al 1990), while Pig-1 function has not yet been clearly established. The three genes are located very close to each other within a DNA segment smaller than 10 kb, with Pig-1 mapping only 813 bp upstream Sgs-4 (Furia et al 1991) and ng-1 mapping about 6 kb downstream Pig-1 (Furia et al 1990). Sgs-4, Pig-1 and ng-1 share common structural and regulatory features, all being small uninterrupted genes that are specifically transcribed within the salivary glands during the larval period of the Drosophila life-cycle.…”

Section: Introductionmentioning

confidence: 98%

Effect of ecd¹ mutation on the expression of genes mapped at the Drosophila melanogaster 3C11-12 intermoult puff

et al. 1992

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The Drosophila melanogaster ecd' mutation causes a severe temperature-sensitive deficiency in the titre of the steroid hormone ecdysone. This mutation was used to investigate the role of ecdysone in both the transcription of the genes mapped at the 3C11-12 intermoult puff region and the puff formation. Thoroughly synchronized ecd' larvae were shifted to the non-permissive temperature at various times of the development; after 24 or 48 h, the levels of the transcripts derived from Sgs-4, Pig-1 and ng-1, the three genes located at the 3C11-12 polytene bands, were determined. The results showed that the levels of the transcripts encoded by Pig-1 and ng-1 are unaffected by the drop in the ecdysone titre occurring in non-permissive conditions whereas the amount of Sgs-4 mRNA is greatly reduced. These data clearly indicate that transcription of the three genes mapped within the puff region is affected differently by the hormone. Furthermore, ecd' larvae cultured at the non-permissive temperature show a prominent puff at the 3C11-12 polytene bands, indicating that ecdysone is not essential for puff induction and that puff size is not simply correlated with high-level Sgs-4 transcription.

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The role of the BR-C locus on the expression of genes located at the ecdysone-regulated 3C puff of Drosophila melanogaster

D’Avino

Crispi

Polito

et al. 1995

Mechanisms of Development

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During the third larval instar, the steroid moulting hormone ecdysone activates three temporally distinct puff sets on the D. melanogaster salivary gland polytene chromosome: the so-called intermoult, early and late puffs. Hormonal regulation of intermoult puffs is quite complex and, so far, largely not understood. In order to further investigate this aspect, we have analysed the effects of mutations in a key regulator of the ecdysone response at the onset of metamorphosis, the Broad-Complex (BR-C) locus, on the expression of genes mapping at the 3C intermoult puff. On the basis of an accurate examination of 3C intermoult gene activity in single, carefully staged, third instar larvae of wild-type and BR-C mutant strains, we were able to subdivide these genes into two groups. Each group is characterised by a different temporal expression profile, so that at the beginning of the wandering stage the transcription of the first group declines as group II transcription is induced. Interestingly, the BR-C locus appears to play a regulatory role in establishing this transcriptional switch. By using mutants of each of the three lethal complementation groups, we precisely defined the role of BR-C functions in this developmental transition and we show that this locus also plays an essential role in the early pre-metamorphic hormonal response.

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Molecular organization of the Drosophila melanogaster Pig-1 gene

Cited by 6 publications

References 16 publications

Nucleotide sequence of the genomic region encompassing Adh and Adh-Dup genes of D. lebanonensis (Scaptodrosophila): Gene expression and evolutionary relationships

Nucleotide sequence of the genomic region encompassing Adh and Adh-Dup genes of D. lebanonensis (Scaptodrosophila): Gene expression and evolutionary relationships

Effect of ecd¹ mutation on the expression of genes mapped at the Drosophila melanogaster 3C11-12 intermoult puff

The role of the BR-C locus on the expression of genes located at the ecdysone-regulated 3C puff of Drosophila melanogaster

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Molecular organization of the Drosophila melanogaster Pig-1 gene

Cited by 6 publications

References 16 publications

Nucleotide sequence of the genomic region encompassing Adh and Adh-Dup genes of D. lebanonensis (Scaptodrosophila): Gene expression and evolutionary relationships

Nucleotide sequence of the genomic region encompassing Adh and Adh-Dup genes of D. lebanonensis (Scaptodrosophila): Gene expression and evolutionary relationships

Effect of ecd1 mutation on the expression of genes mapped at the Drosophila melanogaster 3C11-12 intermoult puff

The role of the BR-C locus on the expression of genes located at the ecdysone-regulated 3C puff of Drosophila melanogaster

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Effect of ecd¹ mutation on the expression of genes mapped at the Drosophila melanogaster 3C11-12 intermoult puff