Molecular networks for the study of TCM Pharmacology

Zhao, Jing; Jiang, Peng; Zhang, Wei‐Dong

doi:10.1093/bib/bbp063

Cited by 187 publications

(131 citation statements)

References 119 publications

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“…As a result, targeting PI3K/AKT and TGF-β/Smad pathways may represent a novel therapeutic method to prevent metastasis without causing side effects. Traditional Chinese medicine (TCM) is of interest to researchers as it induces relatively few side effects and has been clinically used for thousands of years as an important alternative remedy for a variety of diseases (20)(21)(22)(23). TCMs are considered to be multi-component and multi-targeted agents that exert their therapeutic functions holistically (24).…”

Section: Scutellaria Barbata D Don Inhibits Migration and Invasion Omentioning

confidence: 99%

Scutellaria?barbata D. Don inhibits migration and invasion of colorectal cancer cells via suppression of PI3K/AKT and TGF‑β/Smad signaling pathways

et al. 2017

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Abstract. Metastasis is one of the most aberrant behaviors of cancer cells. Patients with cancers, including colorectal cancer (CRC), have a higher risk of tumor recurrence and cancer-related mortality once metastasis is diagnosed. Existing treatment strategies fail to cure cancer mostly due to the onset of metastasis. Therefore, metastasis remains a challenge in cancer treatment. Some complementary and alternative medical therapies using traditional Chinese medicine have been demonstrated to be clinically effective in cancer treatment. Scutellaria barbata D. Don (SB) is a promising medicinal herb. It was previously reported that the ethanol extract of SB (EESB) is able to promote apoptosis, and inhibit cell proliferation and angiogenesis in human colon cancer cells. However, the anticancer effect of SB and the underlying mechanism require further investigation, particularly its role against metastasis. To further elucidate the antimetastatic effect of SB, MTT and Transwell assays were used in the present study to evaluate the effect of EESB on the proliferation, migration and invasion of the CRC cell line HCT-8. In addition, western blot analysis was performed to detect the expression of matrix metalloproteinases (MMPs), cadherins and other metastasis-associated proteins. EESB significantly reduced HCT-8 cell viability and attenuated the migration and invasion ability of HCT-8 cells in a dose-dependent manner. In addition, EESB decreased the expression of MMP-1, MMP-2, MMP-3/10, MMP-9 and MMP-13, and proteins in the phosphoinositide 3-kinase (PI3K)/AKT and transforming growth factor (TGF)-β/Smad pathways, but not the epithelial-mesenchymal transition (EMT)-related factors E-cadherin and N-cadherin. In conclusion, the results suggested that SB inhibits CRC cell metastasis via the suppression of PI3K/AKT and TGF-β/Smad signaling pathways, which may represent a mechanism by which SB exerts an anticancer effect.

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Section: Scutellaria Barbata D Don Inhibits Migration and Invasion Omentioning

confidence: 99%

Scutellaria?barbata D. Don inhibits migration and invasion of colorectal cancer cells via suppression of PI3K/AKT and TGF‑β/Smad signaling pathways

et al. 2017

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“…As a result, multi-target agents with minimal side effects and toxicity are required. Natural products, including TCM, have relatively fewer side effects and have been used clinically to treat various types of diseases including cancer for thousands of years (26)(27)(28). TCM formula is a complex combination of a number of natural products, each of which contains numerous chemical compounds.…”

Section: Discussionmentioning

confidence: 99%

“…Natural products, including traditional Chinese medicine (TCM), have received attention as they have relatively few side effects and have been used clinically for thousands of years as important alternative remedies for a variety of diseases (26)(27)(28). Hedyotis Diffusa Willd (EEHDW) is a medicinal herb widely distributed in northeast Asia.…”

Section: Introductionmentioning

confidence: 99%

Effect of Hedyotis Diffusa Willd extract on tumor angiogenesis

Lin

Wei

et al. 2011

Mol Med Report

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Abstract. Inhibition of tumor angiogenesis has become an attractive target of anticancer chemotherapy. However, drug resistance and cytotoxicity against non-tumor associated endothelial cells limit the long-term use and the therapeutic effectiveness of angiogenesis inhibitors, thus increasing the necessity for the development of multi-target agents with minimal side effects. Traditional Chinese medicine (TCM) formulas, which have relatively fewer side effects and have been used clinically to treat various types of diseases, including cancer, for thousands of years, are considered to be multicomponent and multi-target agents exerting their therapeutic function in a more holistic way. Hedyotis Diffusa Willd (EEHDW) has long been used as an important component in several TCM formulas to treat various types of cancer. Although recently we reported that EEHDW promotes cancer cell apoptosis via activation of the mitochondrial-dependent pathway, the precise mechanism of its tumoricidalactivity still remains to be clarified. In the present study, we investigated the angiogenic effects of the ethanol extract of EEHDW. Cell cycle analysis was perfomed using flow cytometry. Cell viability was analyzed using MTT assay. We found that EEHDW inhibited angiogenesis in vivo in chick embryo chorioallantoic membrane (CAM). In addition, we observed that EEHDW dose-and time-dependently inhibited the prolife ration of human umbilical vein endothelial cells (HUVEC) by blocking the cell cycle G1 to S progression. Moreover, EEHDW inhibited the migration and tube formation of HUVECs. Furthermore, EEHDW treatment down-regulated the mRNA and protein expression levels of VEGF-A in HT-29 human colon carcinoma cells and HUVECs. Our findings suggest that inhibiting tumor angiogenesis is one of the mechanisms by which EEHDW is involved in cancer therapy.

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“…Over the past few years, natural products have attracted extensive attention due to their relatively few side-effects and have been used in clinical practice for thousands of years as important alternative remedies for a variety of diseases (5)(6)(7). Moreover, natural products are an attractive source of new therapeutic candidate compounds, which make an enormous contribution to drug discovery today (8).…”

Section: Introductionmentioning

confidence: 99%

Chemical constituents of volatile oil from Pyrolae herba and antiproliferative activity against SW1353 human chondrosarcoma cells

Cai

et al. 2013

International Journal of Oncology

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Abstract. The objective of the present study was to identify chemical constituents of volatile oil from Pyrolae herba (PHVO) and evaluate the antiproliferative activity of PHVO against SW1353 human chondrosarcoma cells. The volatile oil from Pyrolae herba was prepared by hydrodistillation and characterized by gas chromatography-mass spectroscopy (GC-MS). A total of 12 components in PHVO were identified representing 81.62% of the total integrated chromatographic peaks. The major compounds were found to be n-hexadecanoic acid (29.29%), cedrol (17.08%), 6,10,14-trimethyl-2-pentadecanone (9.59%) and cis-9-octadecadienoic acid (8.23%). The antiproliferative activity of PHVO against SW1353 cells was investigated using MTT assay, flow cytometry and western blot analysis. Our results demonstrated that PHVO inhibited SW1353 cell viability in a dose-and time-dependent manner. Furthermore, PHVO treatment decreased the number of cells entering the S phase and caused a reduction in the expression of cyclin D1, cyclin-dependent kinase (CDK)4 and CDK6, whereas it caused an increase in the expression of p21. PHVO demonstrated potent antitumor activity against SW1353 cells, suggesting its potential use as a therapeutic agent in the treatment of chondrosarcoma. IntroductionChondrosarcoma is the most common primary malignant bone tumor in children and adolescents, comprising almost 60% of all bone sarcomas (1,2). To date, the main treatments for patients with osteosarcoma are surgical resection and chemotherapy (3). However, despite advances in chemotherapy and surgery, the survival rate for osteosarcoma has reached a plateau and 40% of osteosarcoma patients eventually succumb to the disease; thus, there is an urgent need for the development of novel therapeutic agents (4).Over the past few years, natural products have attracted extensive attention due to their relatively few side-effects and have been used in clinical practice for thousands of years as important alternative remedies for a variety of diseases (5-7). Moreover, natural products are an attractive source of new therapeutic candidate compounds, which make an enormous contribution to drug discovery today (8). An analysis of the number of chemotherapeutic agents and their sources indicates that over 60% of approved drugs are derived from natural compounds (9). Several plant-derived compounds are currently successfully employed in disease treatment. For instance, arteannuin, which was isolated from Artemisia annua, has been used for the treatment of malaria and the vinca alkaloid isolated from the periwinkle Catharanthus roseus has been used for the treatment of cancer (10,11). Volatile oils are a type of secondary plant metabolite, and have been shown to exhibit antioxidant and antibacterial activities (12,13).Pyrolae herba listed in Chinese Pharmacopoeia is commonly used in traditional Chinese medicine for the treatment of cancer, hypertension, coronary heart disease and various inflammatory diseases, including rheumatoid arthritis and arthralgia. Pyrolae herba function...

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Molecular networks for the study of TCM Pharmacology

Cited by 187 publications

References 119 publications

Scutellaria?barbata D. Don inhibits migration and invasion of colorectal cancer cells via suppression of PI3K/AKT and TGF‑β/Smad signaling pathways

Scutellaria?barbata D. Don inhibits migration and invasion of colorectal cancer cells via suppression of PI3K/AKT and TGF‑β/Smad signaling pathways

Effect of Hedyotis Diffusa Willd extract on tumor angiogenesis

Chemical constituents of volatile oil from Pyrolae herba and antiproliferative activity against SW1353 human chondrosarcoma cells

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