Molecular Methods for the Detection and Identification of Pseudomonas stutzeri in Pure Culture and Environmental Samples

Bennasar, Antonio; Guasp, Caterina; Lalucat, Jorge

doi:10.1007/s002489900057

Cited by 36 publications

(33 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, a reference strain has been proposed for each genomovar (Table 1). Some relevant strains that were previously assigned to other species are Pseudomonas perfectomarina strain ZoBell (19), Alcaligenes faecalis A15 (380), and Flavobacterium lutescens strain ATCC 27951 (24). Many, but not all, strains have been deposited in publicly recognized culture collections, are available for scientific research, and should be used as reference strains.…”

Section: Discovery and Nomenclatural Problemsmentioning

confidence: 99%

“…DNA methods based on 16S rRNA sequences have been also designed to detect P. stutzeri in DNA extracted directly from environmental samples. Bennasar et al, in 1998, developed PCR primers that were specific to all known genomovars of P. stutzeri at that time (24). This served as a confirmation test, as did amplicon cleavage using the restriction enzyme HindIII or a specific DNA probe targeted at the amplified product (24).…”

Section: Occurrence and Isolation Proceduresmentioning

confidence: 99%

“…Bennasar et al, in 1998, developed PCR primers that were specific to all known genomovars of P. stutzeri at that time (24). This served as a confirmation test, as did amplicon cleavage using the restriction enzyme HindIII or a specific DNA probe targeted at the amplified product (24). Amann et al considered the difficulty of obtaining a DNA probe to cover all of the P. stutzeri strains (5).…”

Section: Occurrence and Isolation Proceduresmentioning

confidence: 99%

“…" Sikorski et al were able to isolate members of P. stutzeri from aquatic habitats and terrestrial ecosystems in a two-step procedure. Firstly, the occurrence of P. stutzeri cells was assessed by a previously designed, slightly modified PCR procedure (24,325). Secondly, the positive samples were screened for P. stutzeri by means of plating on an artificial seawater medium with ethylene glycol, starch, or maltose as the carbon source under aerobic conditions (325).…”

Section: Occurrence and Isolation Proceduresmentioning

confidence: 99%

See 3 more Smart Citations

Biology of Pseudomonas stutzeri

Lalucat

Bennasar

Bosch

et al. 2006

Microbiol Mol Biol Rev

545

418

View full text Add to dashboard Cite

SUMMARY Pseudomonas stutzeri is a nonfluorescent denitrifying bacterium widely distributed in the environment, and it has also been isolated as an opportunistic pathogen from humans. Over the past 15 years, much progress has been made in elucidating the taxonomy of this diverse taxonomical group, demonstrating the clonality of its populations. The species has received much attention because of its particular metabolic properties: it has been proposed as a model organism for denitrification studies; many strains have natural transformation properties, making it relevant for study of the transfer of genes in the environment; several strains are able to fix dinitrogen; and others participate in the degradation of pollutants or interact with toxic metals. This review considers the history of the discovery, nomenclatural changes, and early studies, together with the relevant biological and ecological properties, of P. stutzeri.

show abstract

Section: Discovery and Nomenclatural Problemsmentioning

confidence: 99%

Section: Occurrence and Isolation Proceduresmentioning

confidence: 99%

Section: Occurrence and Isolation Proceduresmentioning

confidence: 99%

Section: Occurrence and Isolation Proceduresmentioning

confidence: 99%

See 2 more Smart Citations

Biology of Pseudomonas stutzeri

Lalucat

Bennasar

Bosch

et al. 2006

Microbiol Mol Biol Rev

545

418

View full text Add to dashboard Cite

show abstract

“…Two primer pairs were used for the PCR amplification: 59-AGAGTTTGATCMTGGCTCAG-39 and 59-CGGTTACCT-TGTTAGGACTTCACC-39 (positions 8-27 and 1488-1511, Escherichia coli numbering system) (Bennasar et al, 1998) and 59-GGCAGGCCTAACACATGCAAGT-39 and 59-GGGCTACCTTGTTACGACTTCACC-39 (positions 41-62 and 1488-1511). The latter primer pair was designed by us according to the 16S rDNA sequences of species in the family Vibrionaceae that are available in the GenBank database.…”

mentioning

confidence: 99%

Vibrio ruber sp. nov., a red, facultatively anaerobic, marine bacterium isolated from sea water

Shieh

2003

International Journal of Systematic and Evolutionary Microbiology

149

View full text Add to dashboard Cite

NoteVibrio ruber sp. nov., a red, facultatively anaerobic, marine bacterium isolated from sea water Institute of Botany, Academy Sinica, Nankang, Taipei, Taiwan A red, heterotrophic, marine bacterium, designated strain VR1 T , was isolated from a sea-water sample collected in the shallow coastal region of Keelung, Taiwan. Cells of the novel strain were facultatively anaerobic, Gram-negative rods that were motile by means of a polar flagellum. The strain grew optimally at 25-30˚C and pH 6-7. Growth required the presence of NaCl, the optimal concentration being about 2 %. The red pigment produced by the cells was identified as prodigiosin. Strain VR1 T grew anaerobically by fermenting glucose and other carbohydrates and producing acids and gases. The strain did not require either vitamins or other organic growth factors for growth. It contained 2-OH-16 : 0 and 3-OH-14 : 0 as the major cellular fatty acids. The DNA G+C content was 45?8 mol%. Phenotypic and chemotaxonomic characterization indicated that strain VR1 T represents a novel species in the genus Vibrio. Strain VR1 T is phenotypically similar to Vibrio gazogenes. However, the reduction of nitrate to nitrite, the ability to utilize D-arabinose, melibiose and L-glycine as sole carbon sources, the inability to utilize sorbitol as a sole carbon source, resistance to O/129 and susceptibility to erythromycin and novobiocin allow differentiation between V. gazogenes and strain VR1 T . The name Vibrio ruber sp. nov. is proposed for the novel species, with strain VR1 T (=CCRC 17186 T =JCM 11486 T ) as the type strain.Marine, halophilic, facultatively anaerobic, Gram-negative, rod-shaped bacteria are currently placed in the genera Vibrio , Photobacterium , Listonella (MacDonell & Colwell, 1985) and Moritella (Urakawa et al., 1998) of the family Vibrionaceae. They are ubiquitous in estuarine, coastal and oceanic waters and in marine sediments (Huq & Colwell, 1995;Simidu & Tsukamoto, 1985;Simidu et al., 1982). They are closely associated with many kinds of marine organisms, from plankton to fish (Cerdà-Cuéllar et al., 1997;MacDonald et al., 1986;Nair et al., 1988;Onarheim et al., 1994;Simidu et al., 1969Simidu et al., , 1971Sochard et al., 1979). Some species are found as symbionts in specialized luminous organs of marine fish and invertebrates (Lee & Ruby, 1994;Leisman et al., 1980;Reichelt et al., 1977;Ruby & Asato, 1993;Ruby & Morin, 1978), whereas many other species are pathogens of humans or marine animals (Blake et al., 1980;Borrego et al., 1996;Egidius et al., 1986;Hada et al., 1984;Ishimaru et al., 1996;Schiewe et al., 1981). These halophilic, facultatively anaerobic, Gram-negative rods are known to include both nitrogen-fixers and denitrifiers (Guerinot & Colwell, 1985;Guerinot et al., 1982;Shieh & Lin, 1992Shieh & Liu, 1996;Shieh & Yang, 1997;Shieh et al., 1989), though the definitions given in Bergey's Manual of Systematic Bacteriology in 1984 indicated that these bacteria include neither nitrogen-fixers nor denitrifiers Baumann & Schubert, 1984;.Vibrio a...

show abstract