Molecular mechanisms of versatile biological activity of interleukin-7

Slyvka, Anton; Okunev, O. V.

doi:10.7124/bc.0008b1

Cited by 7 publications

(6 citation statements)

References 77 publications

(86 reference statements)

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“…Data prove an increase in the level of IL-7 in plasma and lymph in response to the depletion of the T-cell lymphocytes in favor of this mechanism [29]. Among other things, the participation of IL-7 in the development of dendritic cells, natural killers and lymphoid tissue inducer cells has been proved [1]. There is an interesting data regarding a deficit of IL-7 in septic patients [30]: septic patients showed the lowest levels of IL-7.…”

Section: Control Experimentsmentioning

confidence: 91%

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Effect of recombinant human interleukin-7 on Pseudomonas aeruginosa wound infection

Grigorieva¹,

Starosyla²,

Рыбалко³

et al. 2019

Ukr.Biochem.J.

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A wide range of interleukin-7 (IL-7) biological effects suggests that application of appropriate preparations in clinical practice will stimulate immunity in patients with lymphocytic exhaustion or autoimmune diseases. Studies are being conducted for Il-7 based preparations aimed at restoration of the immune system of patients with immunodeficiency of different origins. Pseudomonas aeruginosa is an important pathogen, which causes nosocomial infections in hospitalized patients. Infection factors, affecting the immune status of the host, play a key role. A promising and relevant scientific endeavor is the study of the effect of recombinant as an adjunct therapy in wound infections caused by P. aeruginosa. the aim of this study was to evaluate the effectiveness of rIL-7 use in P. aeruginosa wound infection in mice. The experiments were conducted using a standardized rIl-7 preparation, P. aeruginosa strain and 20 white non-inbred mice. the preparation of rIL-7 after all stages of purification was characterized by the content of ballast proteins and impurities via electrophoresis in a polyacrylamide gel in reducing conditions, and its biological activity was evaluated in the Mtt test by means of proliferation of peripheral blood mononuclear cells. In the mice, the fur was removed, the neck nape was intentionally injured and P. aeruginosa bacteria were injected into the wound of each animal (0.1 ml of suspension with a bacterial cell concentration of 0.08×10 9 cells/ml). Starting from the 2 nd day, bacterial examination of the wound material was carried out daily. Starting from the 3 rd day, the mice (experimental group, n = 10) were intraperitoneally administered 5 μg (0.1 ml) of the rIL-7 preparation. In the control group of animals (n = 10), the rIl-7 preparation was not administered. In 80% of experimental animals (administered the rIl-7 preparation), the healing of wounds and elimination of the pathogen of purulent inflammatory infection P. aeruginosa occurred on the 7 th day. on the 9th day from the beginning of wound infection, wound healing and elimination of P. aeruginosa occurred in all experimental mice. In 60% of mice from the control group (did not receive treatment with rIl-7), wound healing and the elimination of P. aeruginosa occurred on the 9th day. Wound healing and elimination of P. aeruginosa in all mice of the control group occurred on the 14th day. thus, in mice treated with rIl-7, wound healing and elimination of the pathogen occurred 5 days earlier than in mice from the control group . Subsequent studies may be aimed at developing protocols for the treatment of wound infections using an rIl-7 preparation in patients with a compromised immune system therefore, rIl-7 is a promising preparation for the treatment of complex wound infections. k e y w o r d s: recombinant human interleukin-7, Pseudomonas aeruginosa, wound infection, therapy.

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Section: Control Experimentsmentioning

confidence: 91%

“…I nterleukin-7 (IL-7) is a central cytokine of the immune system, which plays an important role in the modulation of T-and B-cell development and T-cell homeostasis [1,2]. The potential and wide range of its effects suggest that IL-7 administration will stimulate immunity in patients with lymphocytic exhaustion and autoimmune diseases.…”

mentioning

confidence: 99%

Effect of recombinant human interleukin-7 on Pseudomonas aeruginosa wound infection

Grigorieva¹,

Starosyla²,

Рыбалко³

et al. 2019

Ukr.Biochem.J.

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“…IL-7 is capable of regulating immune system homeostasis due to its ability to handle balance between processes of apoptosis and proliferation of thymocytes, naïve T cells and memory cells. This is how IL-7 provides consistency of number and functional activity of these populations (Slyvka et al, 2014;Grigorieva et al, 2019;Galkin et al, 2017a). Recombinant proteins, which are intended to be used in medicine for curative purposes, shall correspond to specific requirements, distinguishing them from those proteins used for scientific and research purposes only (Lutsenko et al, 2017;Natochii et al, 2019;Sokol et al, 2020a).…”

Section: Introductionmentioning

confidence: 96%

Development of Human Interleukin-7 Immunoaffinity Purification Method

Gorshunov¹,

Lutsenko²,

Besarab

et al. 2022

J microb biotech food sci

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The article presents the results of the comparative development of different variants of immunoaffinity sorbents for the purification of human IL-7, namely: Sepharose 6B and sol-gel materials based on tetraethoxysilane (TEOS). The optimal conditions for the synthesis of a chromatographic sorbent based on TEOS, which provide acceptable characteristics of the immobilization of a biological substance, were experimentally established. The sorbent based on TEOS and Sepharose 6B provide identical characteristics for the sorption of antibodies after 12 hours of the experiment. To establish rational conditions for immunoaffinity chromatography, various combinations of sorbents, anti-IL-7 monoclonal antibodies, and elution solutions were studied. It was shown that a variant of immunoaffinity chromatography based on TEOS sorbent using 325D2 anti-IL-7 monoclonal antibodies and 4M magnesium chloride solution as eluate, is more effective. Immunochromatographic columns synthesized on the basis of Sepharose 6B and TEOS are characterized by high recovery rates (at least 95%) in a wide range of human IL-7 concentrations (0.05...20 μg/ml), however, the column based on Sepharose 6B is less than stable with repeated use (more than 12 cycles without regeneration). The developed method makes it possible to obtain recombinant human IL-7 with a purity of 98%.

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“…IL-7 is capable of regulating immune system homeostasis due to the ability to maintain a balance between apoptosis and proliferation processes of thymocytes, naïve T-lymphocytes and memory cells, and thus maintaining the consisten cy of abundance and functional activity of these populations [1][2][3].…”

Section: Abstract: Antimicrobial Activity Microbiological Purmentioning

confidence: 99%