Molecular mechanisms of vasculogenesis and embryonic angiogenesis

Flamme, Ingo; Frölich, Thomas; Risau, Werner

doi:10.1002/(sici)1097-4652(199711)173:2<206::aid-jcp22>3.0.co;2-c

Cited by 381 publications

(191 citation statements)

References 64 publications

(8 reference statements)

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“…Previous studies Poole and Coffin, 1988;Burri and Tarek, 1990;deMello et al, 1997) have established that the lung vasculature develops via two independent but concurrent processes, vasculogenesis, in which blood vessels form in situ from angioblasts, and angiogenesis, which involves sprouting of new vessels from existing ones. Recent in vivo and in vitro studies have established that vascular endothelial growth factor (VEGF) and its tyrosine kinase receptors, flk-1 and flt-1, play a critical role in both vasculogenesis and angiogenesis (Breier et al, 1992;Dumont et al, 1995;Flamme et al, 1997). Flk-1 is considered to be the earliest marker of endothelial cell precursors because it is expressed in early mesodermal cells prior to any evidence of endothelial cell differentiation (Yamaguchi et al, 1993).…”

Section: Resultsmentioning

confidence: 99%

Tissue interactions mediate early events in pulmonary vasculogenesis

Gebb

Shannon

2000

Dev. Dyn.

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Extensive study has provided considerable insight into the mechanisms governing branching morphogenesis and developmental maturation of the pulmonary epithelium. The process by which the vascular tree arises in the mesodermal mesenchyme of the developing lung, however, is not known. Because normal epithelial branching and differentiation have been shown to be dependent on interactions with the lung mesenchyme, we hypothesized that the developing pulmonary vasculature is dependent on a reciprocal interaction with pulmonary epithelium. In this study we have defined the temporal and spatial expression of flk-1 mRNA, which encodes an endothelial cell-specific vascular endothelial growth factor (VEGF) receptor, in fetal and neonatal rat lung. Flk-1-positive cells were observed in the lung at every prenatal stage from fetal day 11 through birth, demonstrating that vascularization has been initiated as soon as the lung evaginates from the foregut epithelium. The spatial distribution of vascular precursors was distinct and consistent in early lung (fetal days 11-16): clusters of flk-1-positive cells were localized in the mesenchyme closely apposed to the developing epithelium. This spatial relationship between vascular precursors and the developing epithelium suggested that vascular development in the lung may be dependent on interactions between the two tissue types. To investigate this possibility, day-13 distal lung mesenchyme was cultured in the presence and absence of lung epithelium. Lung mesenchyme cultured in the absence of epithelium degenerated significantly, and few flk-1-positive cells were maintained. In contrast, lung mesenchyme recombined with lung epithelium contained abundant flk-1-positive cells, and their spatial distribution mimicked that observed in vivo. These studies provide the first detailed information regarding the temporal and spatial pattern of pulmonary vascularization in early development and suggest that tissue interactions play an important role in growth and maintenance of the developing lung vasculature. Dev Dyn 2000;217:159 -169.

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Section: Resultsmentioning

confidence: 99%

Tissue interactions mediate early events in pulmonary vasculogenesis

Gebb

Shannon

2000

Dev. Dyn.

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“…5,[9][10][11][12] These lineages were proposed to arise from a common progenitor, the hemangioblast, based on their close temporal and spatial association in the blood islands and on their shared expression of a number of genes. [13][14][15] Some of these genes are required for normal development of both hematopoietic and endothelial cells. [16][17][18][19] Formation of EryPs has been detected in the absence of endothelial cells in the more proximal yolk sac using confocal imaging.…”

Section: Introductionmentioning

confidence: 99%

Maturation and enucleation of primitive erythroblasts during mouse embryogenesis is accompanied by changes in cell-surface antigen expression

Fraser

Isern

Baron

2006

Blood

151

243

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“…[1][2][3][4][5][6][7][8][9] In the developing mouse retina, tip cells are further distinguished from stalk cells by higher levels of transcripts for certain genes including vascular endothelial growth factor (VEGF) receptor (VEGFR) 2 and near selective expression of other genes like platelet derived growth factor B (pdgf-b). 7 Several observations suggest that the tip cell is not a permanent cell fate but rather represents a transient endothelial phenotype that is induced and maintained by VEGF-A stimulation.…”

Section: Introductionmentioning

confidence: 99%