Molecular mechanisms of interactions of plague causative agents with invertebrates

Кутырев, Владимир; Eroshenko, G. A.; Попов, Н. В.; Vidyaeva, N. A.; Konnov, Nikolai P.

doi:10.3103/s0891416809040028

Cited by 16 publications

(11 citation statements)

References 11 publications

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“…In fact, CRgrown salmonellae and P. aeruginosa were found to respond by increasing biofilm production (Klaus and Howard, 2006;Wilson et al, 2007). YP has been shown to produce biofilm under various environmental conditions, including the flea intestine, soil, and exposed surfaces (Kutyrev et al, 2009;Zhou and Yang, 2011) and can inhibit biofilm formation through repression of the diguanylate cyclase gene (hmsT) expression (Sun et al, 2012). The YP strains used in this study were unable to produce biofilm on account of their lacking …”

Section: Discussionmentioning

confidence: 94%

The Effects of Modeled Microgravity on Growth Kinetics, Antibiotic Susceptibility, Cold Growth, and the Virulence Potential of aYersinia pestis ymoA-Deficient Mutant and Its Isogenic Parental Strain

et al. 2013

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Previously, we reported that there was no enhancement in the virulence potential (as measured by cell culture infections) of the bacterial pathogen Yersinia pestis (YP) following modeled microgravity/clinorotation growth. We have now further characterized the effects of clinorotation (CR) on YP growth kinetics, antibiotic sensitivity, cold growth, and YP's virulence potential in a murine model of infection. Surprisingly, none of the aforementioned phenotypes were altered. To better understand why CR did not enhance YP's virulence potential as it did for other bacterial pathogens, a YP DymoA isogenic mutant in the KIM/D27 background strain that is unable to produce the histone-like YmoA protein and influences DNA topography was used in both cell culture and murine models of infection. YmoA represses type three secretion system (T3SS) virulence gene expression in the yersiniae. Similar to our CR-grown parental YP strain data, the CR-grown DymoA mutant induced reduced HeLa cell cytotoxicity with concomitantly decreased Yersinia outer protein E (YopE) and low calcium response V (LcrV) antigen production and secretion. Important, however, were our findings that, although no significant differences were observed in survival of mice infected intraperitoneally with either normal gravity (NG)-or CRgrown parental YP, the DymoA mutant induced significantly more mortality in infected mice than did the parental strain following CR growth. Taken together, our data demonstrate that CR did enhance the virulence potential of the YP DymoA mutant in a murine infection model (relative to the CR-grown parental strain), despite inducing less HeLa cell rounding in our cell culture infection assay due to reduced T3SS activity. Therefore, CR, which induces a unique type of bacterial stress, might be enhancing YP's virulence potential in vivo through a T3SS-independent mechanism when the histone-like YmoA protein is absent. Key Words: Type three secretion system (T3SS)-Low-shear modeled microgravity-High-aspect ratio vessel-Cell culture infection modelsMouse infection model. Astrobiology 13, 821-832.

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Section: Discussionmentioning

confidence: 94%

The Effects of Modeled Microgravity on Growth Kinetics, Antibiotic Susceptibility, Cold Growth, and the Virulence Potential of aYersinia pestis ymoA-Deficient Mutant and Its Isogenic Parental Strain

et al. 2013

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“…В связи с этим в долгосрочной перспективе (до 2020 г.) становится возможным выход из состояния межэпизоотического периода рав-нинных природных очагов Предкавказья, Северного и Северо-Западного Прикаспия: Прикаспийского Северо-Западного степного (14), Волго-Уральского степного (15), Зауральского (Урало-Уильского) степного (17), Дагестанского равнинно-предгорного (03). В 2013-2017 гг.…”

Section: результаты и обсуждениеunclassified

Impact of the Present-Day Climate Changes on the Natural Plague Foci Condition, Situated in the Territory of the Russian Federation and Other CIS Countries

Попов¹,

Безсмертный²,

Udovikov³

et al. 2013

Problemy Osobo Opasnykh Infektsii

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Изменения климата определяют динамику эпизоотической активности природных очагов чумы в XX-XXI cтолетиях. В современный период усиление его континентальности в Северном полушарии может привести к выходу из состояния межэпизоотического периода равнинных степных и полупустынных природных очагов Предкавказья, Северного и Северо-Западного Прикаспия. Наблюдаемое в настоящее время одновременное развитие межэпизоотических периодов в природных очагах чумы различной биоценотической структуры на территории Прикаспийской низменности, дает основание предполагать наличие общей причины, обусловливающей депрессивное состояние паразитарных систем в этом регионе. В частности, одной из главных составляющих этого феномена является изменение статуса малого суслика-прежде основного носителя чумы в данном регионе. В связи с изменением климата основную роль в качестве носителей чумы все более приобретают незимоспящие виды грызунов. В частности, в связи с этим ожидается значительный рост эпизоотического потенциала пустынных природных очагов чумы на территории Казахстана и Средней Азии. По мере дальнейшего понижения температур зимних месяцев, увеличения высоты снегового покрова и роста площади ледников, эпизоотическая активность горных и высокогорных природных очагов должна значительно снизиться. Вместе с тем современные данные по экологии чумного микроба, определяющие возможность его существования в почвенной биоте, подтверждают определяющую роль климатических факторов в механизме энзоотии чумы. Ключевые слова: природные очаги чумы стран СНГ, изменения климата, эпизоотическая активность, эпизоотологический прогноз.

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“…Yersinia pestis strains usually contain three different plasmids, pCad, pFra, and pPst, each playing an important role at a certain stage of plague pathogenesis [1]. Two of these plas mids, namely pFra (92.6 kb) and pPst (9.6 kpb), are species specific.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the nucleotide sequence of a cryptic plasmid from Yersinia pestis strains in the Central-Caucasian high-mountain plague focus

et al. 2015

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An analysis of a 5.4 kb cryptic plasmid detected in the course of whole genome sequencing of the Yersinia pestis medieval biovar strain isolated in the Russian Central Caucasian high mountain plague focus was performed. The identification of the nucleotide sequence of this cryptic plasmid and its structural and functional analysis revealed that it contained eight open reading frames, among which the following genes were identified: the rep gene of a replication protein, the virB6 gene of a type IV secretion system inner mem brane protein, the virB5 gene of the type IV secretion system minor pilin, and a number of genes probably associated with secretion and transport. A general analysis of the pCKF plasmid DNA showed that the ade nine content was 28.34%, the cytosine content was 20.5%, the guanine content was 17.87%, and that of thymine was 33.28%, while the total G+C content appeared to be 38.38%. The G+C content of the chromo some of the Y. pestis strain C 627 is 47.6%, which indicates that the pCKF plasmid was obtained from a microorganism phylogenetically distant from the Yersinia bacteria and any other bacteria from the Entero bacteriaceae family. A comparison of the amino acid sequences of hypothetical proteins encoded by pCKF plasmid with analogous proteins encoded by other bacteria was carried out. The possible contribution of the pCKF plasmid to the maintenance of the most ancient known phylogenetic line of Y. pestis medieval biovar, 2.MED0, was discussed.

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Molecular mechanisms of interactions of plague causative agents with invertebrates

Cited by 16 publications

References 11 publications

The Effects of Modeled Microgravity on Growth Kinetics, Antibiotic Susceptibility, Cold Growth, and the Virulence Potential of aYersinia pestis ymoA-Deficient Mutant and Its Isogenic Parental Strain

The Effects of Modeled Microgravity on Growth Kinetics, Antibiotic Susceptibility, Cold Growth, and the Virulence Potential of aYersinia pestis ymoA-Deficient Mutant and Its Isogenic Parental Strain

Impact of the Present-Day Climate Changes on the Natural Plague Foci Condition, Situated in the Territory of the Russian Federation and Other CIS Countries

Analysis of the nucleotide sequence of a cryptic plasmid from Yersinia pestis strains in the Central-Caucasian high-mountain plague focus

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