Molecular mechanisms of biomass increase in plants

Lima, Marcelo de Freitas; Eloy, Núbia Barbosa; Siqueira, João Antonio; Inzé, Dirk; Hemerly, Adriana S.; Ferreira, Paulo Cavalcanti Gomes

doi:10.1016/j.biori.2017.08.001

Cited by 36 publications

(24 citation statements)

References 122 publications

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“…The modeling revealed that more carbon flux was channeled towards the syntheses of carbohydrates, amino acids, and fibers in KU50 than in HN, which explains the yield gap between both cultivars. Soluble sugars and starch play a central role in carbon metabolism and may have a significant influence on the rate of plant biomass accumulation 56 . Besides, results also showed respiration was higher in HN than KU50.…”

Section: Discussionmentioning

confidence: 99%

Understanding carbon utilization routes between high and low starch-producing cultivars of cassava through Flux Balance Analysis

Chiewchankaset

Siriwat

Suksangpanomrung

et al. 2019

Sci Rep

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Analysis of metabolic flux was used for system level assessment of carbon partitioning in Kasetsart 50 (KU50) and Hanatee (HN) cassava cultivars to understand the metabolic routes for their distinct phenotypes. First, the c onstraint- b ased metabolic m odel of cassava storage r oots, rMeCBM, was developed based on the carbon assimilation pathway of cassava. Following the subcellular compartmentalization and curation to ensure full network connectivity and reflect the complexity of eukaryotic cells, cultivar specific data on sucrose uptake and biomass synthesis were input, and rMeCBM model was used to simulate storage root growth in KU50 and HN. Results showed that rMeCBM-KU50 and rMeCBM-HN models well imitated the storage root growth. The flux-sum analysis revealed that both cultivars utilized different metabolic precursors to produce energy in plastid. More carbon flux was invested in the syntheses of carbohydrates and amino acids in KU50 than in HN. Also, KU50 utilized less flux for respiration and less energy to synthesize one gram of dry storage root. These results may disclose metabolic potential of KU50 underlying its higher storage root and starch yield over HN. Moreover, sensitivity analysis indicated the robustness of rMeCBM model. The knowledge gained might be useful for identifying engineering targets for cassava yield improvement.

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Section: Discussionmentioning

confidence: 99%

Understanding carbon utilization routes between high and low starch-producing cultivars of cassava through Flux Balance Analysis

Chiewchankaset

Siriwat

Suksangpanomrung

et al. 2019

Sci Rep

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“…Therefore, genetic engineering of vegetative tissues to accumulate higher triacylglycerol (TAG) levels has drawn attention (Durrett, Benning and Ohlrogge, 2008; Qazi, Paranjpe and Bhargava, 2012; Napier et al , 2014a; Kumar, 2020). Since C4 grasses can be grown on marginal lands not suitable for most food crops, the high biomass producing vegetative tissues of sorghum, miscanthus, and sugarcane are desirable targets for engineering efforts aimed at producing and/or storing lipids (Carlsson et al , 2011; Sanjaya et al , 2011; Weselake, 2016a; Lima et al , 2017). For example, stem tissues engineered to divert energy from nonstructural carbohydrates into the lipid biosynthesis pathway through strategies such as increasing the supply of FAs, increasing TAG assembly activities, and blocking TAG breakdown pathways have resulted in higher amounts of TAGs accumulating in vegetative tissues (Papini-Terzi et al , 2009; Waclawovsky et al , 2010b; Sanjaya et al , 2011; Qazi et al , 2014; Sekhon et al , 2016).…”

Section: Introductionmentioning

confidence: 99%

Sorghum bicolorcultivars have divergent and dynamic gene regulatory networks that control the temporal expression of genes in stem tissue

Arachchilage

Mullet

Marshall‐Colón

2020

Preprint

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The genetic engineering of value-added traits such as the accumulation of bioproducts 21 in high biomass C4 grass stems is one promising strategy to make plant-derived biofuels more 22 economical for industrial use. A first step toward achieving this goal is to identify stem-specific 23 promoters that can drive the expression of genes of interest with good temporal and spatial 24 specificity. However, a comprehensive characterization of the spatial-temporal regulatory 25 elements of stem tissue-specific promoters for C4 grasses has not been reported. Therefore, we 26 performed an in-silico analysis on Sorghum bicolor cv BTx623 transcriptomes from multiple 27 tissues over development to identify stem-expressed genes. The analysis identified 10 genes that 28 are "Always-On-Stem-Specific," 59 genes that are "Temporally-Stem-Specific during early 29 development," and 21 genes that are "Temporally-Stem-Specific during late development." 30 Promoter analysis revealed common and/or unique cis-regulatory elements in promoters of genes within each of the three categories. Subsequent gene regulatory network (GRN) analysis revealed 32 that different transcriptional regulatory programs are responsible for the temporal activation of the 33 stem-expressed genes. The analysis of temporal stem GRNs between sweet (cv Della) and grain 34 (cv BTx623) sorghum varieties revealed genetic variation that could influence the regulatory 35 landscape. This study provides new insights about sorghum stem biology, and information for 36 future genetic engineering efforts to fine-tune the spatial-temporal expression of transgenes in C4 37 grass stems. 38 39 40 High biomass grasses, such as sorghum (Sorghum bicolor), miscanthus (Miscanthus x 41 giganteus), switchgrass (Panicum virgatum), and sugarcane (Saccharum sp.), offer an abundant 42 and renewable source of biomass for biofuels production (McLaughlin and Kszos, 2005; Rooney 43 et al.

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“…This strategy is in agreement with the literature; the cold acclimation is accompanied by a decrease in growth (reviewed in [38]) and since sugars are not necessary to be synthesized to support growth, starch, as a source of energy for further usage, is synthesized preferentially at the expense of monosaccharides. Indeed, a negative correlation between plant growth and starch accumulation in plant leaves has been reported (reviewed in [39]). Further, these changes are accompanied by downregulation of photosynthetic activity, as observed in ARK (Figures 3 and 4), the downregulation being driven by reprogramming of gene expression (reviewed in [40]).…”

Section: Discussionmentioning

confidence: 99%

Analysis of Cold-Developed vs. Cold-Acclimated Leaves Reveals Various Strategies of Cold Acclimation of Field Pea Cultivars

et al. 2019

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Peas (Pisum sativum L.) belong among the world's oldest domesticated crops, serving as a source of proteins, complex carbohydrates, vitamins and minerals. Autumn sowing allows a higher biomass production as well as the avoidance of the drought and heat stresses of late spring. However, the character of European continental winters limits plant growth and development through cold stress. This work sought parameters that reflect the cold tolerance of pea plants and consequently to suggest an afila-type pea cultivar with resilience to European continental winters. For this purpose, we employed indoor remote sensing technology and compared the 22-day-long acclimation to 5 • C of four pea cultivars: Arkta, with normal leaves and the known highest cold resistance to European continental winters, and Enduro, Terno and CDC Le Roy, all of the afila type. Besides evaluation of shoot growth rate and quenching analysis of chlorophyll fluorescence (ChlF) by imaging methods, we measured the chlorophyll content and ChlF induction with a nonimaging fluorometer. Here we show that the acclimation to cold of the Arkta exhibits a different pattern than the other cultivars. Arkta showed the fastest retardation of photosynthesis and shoot growth, which might be part of its winter survival strategy. Terno, on the other hand, showed sustained photosynthetic performance and growth, which might be an advantageous strategy for spring. Surprisingly, Enduro showed sustained photosynthesis in the stipules, which transferred and acclimated to 5 • C (cold-acclimated). However, of all the cultivars, Enduro had the strongest inhibition of photosynthesis in new stipules that developed after the transition to cold (cold-developed). We conclude that the parameters of ChlF spatial imaging calculated as averages from whole plants are suboptimal for the characterization of various cold acclimation strategies. The most marked changes were obtained when the new cold-developed leaves were analyzed separately from the rest of the plant.2 of 16 minerals [3]. In an effort to improve grain yield, seed protein content, disease resistance, or the tolerance to various stresses, pea plants have been bred into different types. In 1965, Goldenberg discovered the afila type, whose leaves were transformed into tendrils that tend to twine together and so the plants offer mutual support to each other [4]. The leaves transformed into tendrils thus brought benefits in the form of a pea canopy with increased lodging resistance.A further important trait is represented by the adaptability of plants to different climates. Various species of overwintering plants have developed adaptive responses to the seasonal changes in the weather [5][6][7][8]. They sense the upcoming cold period through the perception of environmental signals. The process of sensing an upcoming period with low temperatures leading to changes at the level of gene expression, cellular water management, plant metabolism and physiology is known as cold acclimation. Cold acclimation occurs in species from temp...

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Molecular mechanisms of biomass increase in plants

Cited by 36 publications

References 122 publications

Understanding carbon utilization routes between high and low starch-producing cultivars of cassava through Flux Balance Analysis

Understanding carbon utilization routes between high and low starch-producing cultivars of cassava through Flux Balance Analysis

Sorghum bicolorcultivars have divergent and dynamic gene regulatory networks that control the temporal expression of genes in stem tissue

Analysis of Cold-Developed vs. Cold-Acclimated Leaves Reveals Various Strategies of Cold Acclimation of Field Pea Cultivars

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