Molecular Mechanisms of Apical and Basolateral Sorting in Polarized Epithelial Cells

Weisz, Ora A.; Fölsch, Heike

doi:10.1007/978-3-030-52780-8_5

Cited by 4 publications

(1 citation statement)

References 144 publications

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“…To ensure this, polarized epithelial cells continuously sort membrane receptors and adhesion molecules to either surface domain ( Rodriguez-Boulan et al. , 2004 ; Weisz and Rodriguez-Boulan, 2009 ; Weisz and Fölsch, 2016 ). However, little is known about the involvement or behavior of the polarized sorting machinery during epithelial wound healing.…”

Section: Introductionmentioning

confidence: 99%

Novel function for AP-1B during cell migration

Kell

Ang

Pigati

et al. 2020

MBoC

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The epithelial cell-specific clathrin adaptor AP-1B has a well-established role in polarized sorting of cargos to the basolateral membrane. Here we show that β1 integrin was dependent on AP-1B and its co-adaptor, autosomal recessive hypercholesterolemia protein (ARH), for sorting to the basolateral membrane. We further demonstrate an unprecedented role for AP-1B at the basal plasma membrane during collective cell migration of epithelial sheets. During wound healing, expression of AP-1B (and ARH in AP-1B-positive cells) slowed epithelial-cell migration. We show that AP-1B colocalized with β1 integrin in focal adhesions during cell migration using confocal microscopy and total internal reflection fluorescence (TIRF) microscopy on fixed specimens. Further, AP-1B labeling in cell protrusion was distinct from labeling for the endocytic adaptor complex AP-2. Using stochastic optical reconstruction microscopy (STORM) we identified numerous AP-1B-coated structures at or close to the basal plasma membrane in cell protrusions. In addition, immuno-electron microscopy (EM) showed AP-1B in coated pits and vesicles at the plasma membrane during cell migration. Lastly, qRT-PCR analysis of human epithelial-derived cell lines revealed a loss of AP-1B expression in highly migratory metastatic cancer cells suggesting that AP-1B's novel role at the basal plasma membrane during cell migration might be an anti-cancer mechanism. [Media: see text] [Media: see text]

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Section: Introductionmentioning

confidence: 99%

Novel function for AP-1B during cell migration

Kell

Ang

Pigati

et al. 2020

MBoC

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GOPC facilitates the sorting of syndecan-1 in polarized epithelial cells

Ford

Burd

2022

MBoC

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Cargo sorting at the trans-Golgi network at a glance

Ford

Parchure

Blume

et al. 2021

Journal of Cell Science

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The Golgi functions principally in the biogenesis and trafficking of glycoproteins and lipids. It is compartmentalized into multiple flattened adherent membrane sacs termed cisternae, which each contain a distinct repertoire of resident proteins, principally enzymes that modify newly synthesized proteins and lipids sequentially as they traffic through the stack of Golgi cisternae. Upon reaching the final compartments of the Golgi, the trans cisterna and trans-Golgi network (TGN), processed glycoproteins and lipids are packaged into coated and non-coated transport carriers derived from the trans Golgi and TGN. The cargoes of clathrin-coated vesicles are chiefly residents of endo-lysosomal organelles, while uncoated carriers ferry cargo to the cell surface. There are outstanding questions regarding the mechanisms of protein and lipid sorting within the Golgi for export to different organelles. Nonetheless, conceptual advances have begun to define the key molecular features of cargo clients and the mechanisms underlying their sorting into distinct export pathways, which we have collated in this Cell Science at a Glance article and the accompanying poster.

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Molecular Mechanisms of Apical and Basolateral Sorting in Polarized Epithelial Cells

Cited by 4 publications

References 144 publications

Novel function for AP-1B during cell migration

Novel function for AP-1B during cell migration

GOPC facilitates the sorting of syndecan-1 in polarized epithelial cells

Cargo sorting at the trans-Golgi network at a glance

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