GOPC facilitates the sorting of syndecan-1 in polarized epithelial cells

Ford, Charlotte; Burd, Christopher G.

doi:10.1091/mbc.e22-05-0165

Cited by 2 publications

(2 citation statements)

References 75 publications

(109 reference statements)

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“…In this context, it is important to note that several environmental cues and upstream regulators can induce autophagy by facilitating the assembly of the Beclin 1–Vps34 core complex, which is crucial for phagophore formation . One of these upstream autophagy regulators is PIST, also known as GOPC (Golgi-associated PDZ and coiled-coil motif-containing protein), which plays key roles in signal transduction pathways. − Additionally, PIST is involved in the intracellular trafficking of various cellular proteins, such as Cadherin 23, cystic fibrosis transmembrane conductance regulator (CFTR), and metabotropic glutamate receptor 5 (mGluR5). − …”

Section: Introductionmentioning

confidence: 99%

Role of Macrophage PIST Protein in Regulating Leishmania major Infection

Banerjee,

Gadpayle,

Samanta

et al. 2024

ACS Infect. Dis.

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PDZ protein interacting specifically with Tc10 or PIST is a mammalian trans-Golgi resident protein that regulates subcellular sorting of plasma membrane receptors. PIST has recently emerged as a key player in regulating viral pathogenesis. Nevertheless, the involvement of PIST in parasitic infections remains unexplored. Leishmania parasites infiltrate their host macrophage cells through phagocytosis, where they subsequently multiply within the parasitophorous vacuole (PV). Host cell autophagy has been found to be important in regulating this parasite infection. Since PIST plays a pivotal role in triggering autophagy through the Beclin 1−PI3KC3 pathway, it becomes interesting to identify the status of PIST during Leishmania infection. We found that while macrophage cells are infected with Leishmania major (L. major), the expression of PIST protein remains unaltered; however, it traffics from the Golgi compartment to PV. Further, we identified that in L. major-infected macrophage cells, PIST associates with the autophagy regulatory protein Beclin 1 within the PVs; however, PIST does not interact with LC3. Reduction in PIST protein through siRNA silencing significantly increased parasite burden, whereas overexpression of PIST in macrophages restricted L. major infectivity. Together, our study reports that the macrophage PIST protein is essential in regulating L. major infectivity.

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Section: Introductionmentioning

confidence: 99%

Role of Macrophage PIST Protein in Regulating Leishmania major Infection

Banerjee,

Gadpayle,

Samanta

et al. 2024

ACS Infect. Dis.

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“…In this context, it is worth mentioning that based on the environmental que several cofactors or upstream regulators induces autophagy by directing the formation of the Beclin 1-Vps34 core complex which further drives the phagophore construction (Kang et al, 2011). Among these upstream autophagy regulators, PDZ domain protein interacting specifically with Tc-10 (PIST) which is also known as GOPC (Golgi-Associated PDZ and Coiled-Coil Motif-Containing Protein) plays important roles in signal transduction pathway (Ford & Burd, 2022; Koliwer et al, 2015; Neudauer et al, 2001; Wente et al, 2005). Additionally, PIST has been found to be involved in the trafficking of different cellular proteins like Cadherin 23, cystic fibrosis transmembrane conductance regulator (CFTR), and metabotropic glutamate receptor 5 (mGluR5) (Cushing et al, 2008; Klüssendorf et al, 2021; Pelaseyed & Hansson, 2011; Xu et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

A novel role of macrophage PIST protein in regulatingLeishmania majorinfection

Banerjee

Gadpayle

Das

et al. 2023

Preprint

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PDZ protein interacting specifically with Tc10 or PIST is a mammalian trans-Golgi resident protein that regulates subcellular sorting of plasma membrane receptors. PIST has recently been found to play an important role in regulating viral pathogenesis. Alteration in PIST expression is linked to the reprogramming of cell surface receptors which is crucial in determining herpes simplex virus1 infection. In this context, PIST is crucial in triggering autophagy via Beclin 1 -PI3KC3 pathway. However, there is complete lack in our knowledge on the role of this protein in any parasitic infection. Leishmania parasites infect their host macrophage cells via phagocytosis and their survival within the parasitophorous compartment has recently been found to be dependent on host autophagy by a yet to be identified mechanism. Using Leishmania major (L. major)- macrophage infection model system we, for the first-time report here that in infected macrophages Golgi resident PIST protein migrates towards the parasite containing compartment. We have also found that PIST associates with Beclin 1, however, not with LC3 within the L. major parasite containing compartment of infected macrophages. Further, we performed genetic ablation of PIST by siRNA and observed that knockdown of macrophage PIST in turn helps in parasite replication. Contrary to this, overexpression of PIST in macrophages restricted the multiplication of L. major. Collectively, our study for the first time reveals that PIST is essential in regulating intracellular parasite, L. major infection within macrophage cells.

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GOPC facilitates the sorting of syndecan-1 in polarized epithelial cells

Cited by 2 publications

References 75 publications

Role of Macrophage PIST Protein in Regulating Leishmania major Infection

Role of Macrophage PIST Protein in Regulating Leishmania major Infection

A novel role of macrophage PIST protein in regulatingLeishmania majorinfection

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