Molecular mechanism of anti-inflammatory activity of Pluchea indica leaves in macrophages RAW 264.7 and its action in animal models ofinflammation

Buapool, Doungnapa; Mongkol, Nadtaya; Chantimal, Jirapa; Roytrakul, Sittiruk; Srisook, Ekaruth; Srisook, Kloakwan

doi:10.1016/j.jep.2013.01.014

Cited by 50 publications

(43 citation statements)

References 39 publications

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“…In this study, the proliferation and migration of oral mucosal cells treated with Pluchea indica extract and NPs containing Pluchea indica leaf extract were assessed to investigate the in vitro wound healing properties. The anti-inflammatory, anti-nociceptive, antituberculosis and antioxidant activities of Pluchea indica leaf ethanol extract were reported (Sen et al 1993;Mohamad et al 2011;Buapool et al 2013;Suriyaphan 2014). In Asian traditional medicines, a poultice of leaves is applied externally on top of the wound to treat ulcers and sooth sores (Wiart 2006).…”

Section: Discussionmentioning

confidence: 99%

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Wound healing activity of Pluchea indica leaf extract in oral mucosal cell line and oral spray formulation containing nanoparticles of the extract

Buranasukhon

Athikomkulchai

Tadtong

et al. 2017

Pharmaceutical Biology

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Context: Pluchea indica (L.) Less (Asteraceae) is an herb used as a traditional medicine for wound healing. The chemical compounds found in Pluchea indica leaves are phenolic acids, flavonoids, anthocyanins and carotenoids.Objective: This study investigates the effect of Pluchea indica leaf ethanol extract and its nanoparticles (NPs) on cytotoxicity, cell survival and migration of human oral squamous carcinoma cell line. Materials and methods: Cell viability was measured using MTT assay to assess the effect of Pluchea indica leaf extract and NPs (1-500 lg/mL) on cytotoxicity and cell survival. The effect of Pluchea indica leaf extract and NPs on cell migration was determined by scratch assay. The % relative migration was calculated after 24, 48 and 72 h of treatment.Results: The sizes of Pluchea indica leaf extract NPs were in a range of nanometers. NPs possessed negative charge with the polydispersity index (PDI) smaller than 0.3. After the treatment for 24, 48 and 72 h, Pluchea indica leaf extract had IC 50 value of 443.2, 350.9 and 580.5 lg/mL, respectively, whereas the IC 50 value of NPs after the treatment for 24, 48 and 72 h were 177.4, 149.2 and 185.1 lg/mL, respectively. The % relative migration of cells was significantly increased when the cells were treated with 62.5 and 125 lg/mL of the extract and 62.5 lg/mL of NPs. Discussion and Conclusions: NPs increased cytotoxicity of the Pluchea indica leaf extract, increased the migration of cells at low concentration and increased colloidal stability of the extract in an oral spray formulation. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

“…In Thai traditional medicines, the leaf was used to treat diabetes, gallstone, haemorrhoids, fever and to heal wounds. According to the studies, Pluchea indica leaf extract exhibited anti-tuberculosis activity and potent anti-inflammatory activity (Mohamad et al 2011;Buapool et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Wound healing activity of Pluchea indica leaf extract in oral mucosal cell line and oral spray formulation containing nanoparticles of the extract

Buranasukhon

Athikomkulchai

Tadtong

et al. 2017

Pharmaceutical Biology

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“…Multiple lines of evidence indicate MAPKs signaling pathways play a role in regulation of iNOS and COX-2 expression (Chen et al, 1999;Chun et al, 2012;Buapool et al, 2013). MAPKs activation requires the phosphorylation of threonine and tyrosine residues (Chun et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

“…Nuclear translocation of NF-κB was determined from nuclear protein which extracted by the method of Buapool et al (2013). Protein concentration was determined with a Quick Start Bradford protein assay kit (Bio-Rad, USA.)…”

Section: Western Blot Analyses For Protein Expressionmentioning

confidence: 99%

Bioassay-guided isolation and mechanistic action of anti-inflammatory agents from Clerodendrum inerme leaves

Srisook

Nachaiyo

et al. 2015

Journal of Ethnopharmacology

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“…It is usually consumed as fresh vegetables, decoction, and topical medication. [6][7][8][9][10] Staphylococcus epidermidis (S. epidermidis) and Pseudomonas aeruginosa (P. aeruginosa) are two most prevalent bacterial pathogens infecting respiratory disease, urinary tract, eyes, and burn wounds. Their presence was associated with poor patient outcomes.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of Pluchea indica and Piper betle Ethanol Extract on Staphylococcus epidermidis and Pseudomonas aeruginosa

Simanjuntak¹,

Yuniarni²,

Prayugo³

2016

PCPR

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Medicinal plants are widely used for the treatment of different infectious diseases. This study was aimed to investigate antibacterial activity of Pluchea indica (P. indica) and Piper betle (P.betle) ethanol extract on Staphylococcus epidermidis (S. epidermidis) and Pseudomonas aeruginosa (P. aeruginosa) using agar disk diffusion method. Ethanol extract of Piper betle showed more potent antibacterial activity than P. indica against S. Epidermidis with the highest inhibiton zone at 30.71 mm and 21.73 mm from 1 mg/ml concentration, respectively. In contrast, against P. aeruginosa, the ethanol extract of P. indica was more potent than P. betle with 21.44 mm and 20.12 mm of inhibition zone on 1 mg/ml concentration, respectively. There was no increased effect from the combination of these two extracts against these bacteria. When comparing the antibacterial activity of these extract with tetracycline as the standard, we found that antibacterial activity of P. indica at the concentration of 0.9 mg/ ml was comparable with that of tetracycline at concentration of 12.52 μg/ml, while P. betle needed 0.3 mg/ml concentration to had similar activity with 10.51 μg/ml of tetracycline. In conlusion, the antibacterial activity of ethanol extracts of P. indica and P. betle indicated that these extract had sufficient potential to warrant further examination and development as a new antibacterial agent.

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Molecular mechanism of anti-inflammatory activity of Pluchea indica leaves in macrophages RAW 264.7 and its action in animal models ofinflammation

Cited by 50 publications

References 39 publications

Wound healing activity of Pluchea indica leaf extract in oral mucosal cell line and oral spray formulation containing nanoparticles of the extract

Wound healing activity of Pluchea indica leaf extract in oral mucosal cell line and oral spray formulation containing nanoparticles of the extract

Bioassay-guided isolation and mechanistic action of anti-inflammatory agents from Clerodendrum inerme leaves

Antibacterial Activity of Pluchea indica and Piper betle Ethanol Extract on Staphylococcus epidermidis and Pseudomonas aeruginosa

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