Molecular mechanism about lymphogenous metastasis of hepatocarcinoma cells in mice

Abstract

Secretion of MMPs which was associated with metastatic ability of Hca-F and Hca-P tumor cells depends on the environment of lymph nodes. The increased expression of Fas ligand protein of Hca-F tumor cells with high lymphogenous metastatic potential in lymph nodes may help tumor cells escape from being killed by host lymphocytes.

Inhibition of basigin expression in glioblastoma cell line via antisense RNA reduces tumor cell invasion and angiogenesis

“…4,5 Previous studies demonstrated that EMMPRIN concentrated on the surfaces of most tumor cells, promoted invasion of tumor cells by stimulating stromal cells to produce elevated levels of several matrix metalloproteinase (MMPs) 6,7 which play very important roles in several aspects of tumor progression, including growth, invasion, metastasis, and angiogenesis. [8][9][10] Moreover, supporting its key role in the processes of tumorigenesis and metastasis, EMMPRIN was reported as one of the most constantly upregulated mRNA in metastatic cells. 11 Our interest in gliomas let us to consider the role of CD147 in glioma invasion, metastasis, and angiogenesis.…”

Osteoglycin expression influences gelatinases activity of murine hepatocarcinoma cells cultured with extract of lymph node

“…As the main mediators of extracellular matrix degradation, gelatinases are suggested to play important roles in tumor metastasis [8] . Our early work showed that the quantity of gelatinases produced by Hca-P and Hca-F cells were closely associated with the metastatic potential of the tumor cells when cultured with extract of lymph node [13] . In the present study, high expression of osteoglycin after osteoglycin gene transfection attenuated the secretion of gelatinases (Pro-MMP-9, MMP-9 active, Pro-MMP-2 and MMP-2 active) in Hca-F cells cultured with extract of lymph node, and effective suppression of osteoglycin expression by osteoglycin shRNA enhanced the secretion of gelatinases (Pro-MMP-9, MMP-9 active, Pro-MMP-2 and MMP-2 active) in Hca-P cells cultured with extract of lymph node, suggesting that the regulation of gelatinases activity might be one of the mechanisms that osteoglycin contribute to lymphatic metastasis suppression.…”

Proteomic research progress in lymphatic metastases of cancers

“…Our previous work indicates that two mouse hepatocarcinoma ascites syngeneic cell lines, Hca-F with a high LNM rate of >75% and Hca-P with a low LNM rate of <25%, metastasise only to the organ of lymph node, and do not disseminate to other organs, which have been shown to be excellent cell models for studying tumour malignancy contributions to LNM [7]. Previous proteomics work from our group, which used two-dimensional difference in gel electrophoresis (2-D DIGE) coupled high-performance liquid chromatography/HPLC-electro-spray ionisation mass spectrometry (HPLC-nESI-MS/MS) analysis, indicated that 113 proteins were differentially expressed between the two cell lines.…”