Proteomic research progress in lymphatic metastases of cancers

Abstract

Lymph node metastasis (LNM) is recognised as an important factor involved in malignant tumour progression by interfering with a favourable prognosis. It is involved in a variety of cancers. Proteins are believed to play important roles in the LNM of cancers. The rapid achievements of state-of-the-art proteomic techniques have emerged as the key technologies successfully applied to identify markers for cancers at high-throughput level by providing novel targets and creating possible therapeutic interventions in cancer research. This review summarises recent progress in proteomic research in hepatocarcinoma, gastric cancer, oesophageal cancer, colorectal cancer, breast cancer, lung cancer and nasopharyngeal cancer. Actin, heat-shock proteins (HSPs), annexins, cytokeratin 10 (CK10), CK19, protein gene product 9.5 (PGP9.5) and protein disulfide isomerase (PDI) are the most common proteins in lymphatic metastases of cancers revealed by proteomic and protein functional studies. Other protein candidates specifically associated with LNMs of certain cancers are also summarised and discussed.

CRKL knockdown promotes in vitro proliferation, migration and invasion, in vivo tumor malignancy and lymph node metastasis of murine hepatocarcinoma Hca-P cells

“…Recently, we found CRKL was linked to the lymphatic metastasis of hepatocarcinoma [23]. Using murine hepatocarcinoma Hca-P cell line, an ideal cell line for mimicking the initial (low) and specific lymph node metastasis (LNM) of hepatocarcinoma in vitro and in vivo [2,4,[23][24][25][26][27][28], we found CKLP overexpression effectively inhibits the in vitro proliferation, migration and invasion capacities of Hca-P cells [23]. Herein, the potential tumor suppressor role of CRKL in Hca-P was further validated by RNAi silencing combined with in vitro proliferation, migration and invasion assays and with in vivo tumorigenicity and LNM rate and level determinations.…”

“…Lymph node metastasis (LNM) is associated with a 50% reduction in a favorable prognosis [3]. The study on LNM in cancers contributes to early metastasis detection and track cancer progression [4]. Characterized with high recurrence, metastasis and poor prognosis [5], hepatocarcinoma is one of the leading causes of cancer-related deaths [6].…”

“…Its overexpression in Hca-P, an initial (low) and specific lymph node metastasis (LNM) murine hepatocarcinoma cell line [2,4,[23][24][25][26][27][28], suppresses the in vitro proliferation, migration and invasion abilities of Hca-P cells. In current work, we continuously validated the potential tumor suppressor role of Our previous study (Biomed Pharmacother 2015;69:11) demonstrated that the over-expression of CRKL, a chicken tumor virus number 10 regulator of kinase-like protein, suppresses in vitro proliferation, invasion and migration of murine hepatocarcinoma Hca-P cell, a murine HCC cell with lymph node metastatic (LNM) rate of $25%.…”

Endoscopic Ultrasound-Guided Transesophageal Thoracic Duct Puncture in a Swine Model: A Survival Study

“…EUS-guided thoracic duct puncture and lymph analysis may also allow proteomic profiling of lymph for potential biomarkers for malignancy. 19 Improving access to the thoracic duct may open a new source for emerging molecular studies on EUS-fine needle aspirate material, which in the future may provide diagnostic and prognostic information that is beyond routine conventional cytology. 20 These findings of our survival study indicate that EUSguided transesophageal thoracic duct puncture is a safe procedure.…”

3,4-Methylenedioxy-β-nitrostyrene inhibits adhesion and migration of human triple-negative breast cancer cells by suppressing β1 integrin function and surface protein disulfide isomerase

“…PDI expression is up-regulated in a variety of cancer cells, its activity supports the survival of several cancers, including breast cancer [57]. PDI also promotes cancer migration and invasion [17,58,59]. Moreover, lower PDI expression was observed to be significantly associated with higher overall survival rate of patients with breast cancer [60].…”