Molecular markers unravel intraspecific and interspecific genetic variability in Plantago ovata and some of its wild allies

Kotwal, Shivanjali; Dhar, Manoj K.; Kour, Balbir; Raj, Kuldeep; Kaul, Sanjana

doi:10.1007/s12041-013-0240-4

Cited by 6 publications

(4 citation statements)

References 16 publications

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“…We have recently reported the cross-genus amplification of several SSR markers (based on genus Malus and Phaseolus ) in several accessions of P . ovata and different species of the genus Plantago [ 67 ].…”

Section: Resultsmentioning

confidence: 99%

De Novo Transcriptome Analysis of Medicinally Important Plantago ovata Using RNA-Seq

et al. 2016

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Plantago ovata is an economically and medicinally important plant of the family Plantaginaceae. It is used extensively for the production of seed husk for its application in pharmaceutical, food and cosmetic industries. In the present study, the transcriptome of P. ovata ovary was sequenced using Illumina Genome Analyzer platform to characterize the mucilage biosynthesis pathway in the plant. De novo assembly was carried out using Oases followed by velvet. A total of 46,955 non-redundant transcripts (≥100 bp) using ~29 million high-quality paired end reads were generated. Functional categorization of these transcripts revealed the presence of several genes involved in various biological processes like metabolic pathways, mucilage biosynthesis, biosynthesis of secondary metabolites and antioxidants. In addition, simple sequence-repeat motifs, non-coding RNAs and transcription factors were also identified. Expression profiling of some genes involved in mucilage biosynthetic pathway was performed in different tissues of P. ovata using Real time PCR analysis. The study has resulted in a valuable resource for further studies on gene expression, genomics and functional genomics in P. ovata.

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Section: Resultsmentioning

confidence: 99%

De Novo Transcriptome Analysis of Medicinally Important Plantago ovata Using RNA-Seq

et al. 2016

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“…The first group, devoid of nitrogen in their backbone, consists of etidronate, clodronate and tiludronate, whereas the second group containing nitrogen, consists of alendronate, ibandronate, olpadronate, pamidronate, risendronate and zolendronate (van Beek et al, 2003). BPs play an essential role in bone disease treatment, including osteoporosis, Paget's disease, decreasing excessive bone resorption (Kotwal et al, 2013;Fleisch, 1991). Furthermore, BPs have pro-apoptotic potential by inducing osteoclast apoptosis, which has led to their application in skeletal associated disorders (Papapoulos, 2008;Zeman and Shipman, 1997).…”

Section: Bisphosphonatesmentioning

confidence: 99%

Could drugs inhibiting the mevalonate pathway also target cancer stem cells?

Likus

Siemianowicz

Bieńk

et al. 2016

Drug Resistance Updates

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Understanding the connection between metabolic pathways and cancer is very important for the development of new therapeutic approaches based on regulatory enzymes in pathways associated with tumorigenesis. The mevalonate cascade and its rate-liming enzyme HMG CoA-reductase has recently drawn the attention of cancer researchers because strong evidences arising mostly from epidemiologic studies, show that it could promote transformation. Hence, these studies pinpoint HMG CoA-reductase as a candidate proto-oncogene. Several recent epidemiological studies, in different populations, have proven that statins are beneficial for the treatment-outcome of various cancers, and may improve common cancer therapy strategies involving alkylating agents, and antimetabolites. Cancer stem cells/cancer initiating cells (CSC) are key to cancer progression and metastasis. Therefore, in the current review we address the different effects of statins on cancer stem cells. The mevalonate cascade is among the most pleiotropic, and highly interconnected signaling pathways. Through G-protein-coupled receptors (GRCP), it integrates extra-, and intracellular signals. The mevalonate pathway is implicated in cell stemness, cell proliferation, and organ size regulation through the Hippo pathway (e.g. Yap/Taz signaling axis). This pathway is a prime preventive target through the administration of statins for the prophylaxis of obesity-related cardiovascular diseases. Its prominent role in regulation of cell growth and stemness also invokes its role in cancer development and progression. The mevalonate pathway affects cancer metastasis in several ways by: (i) affecting epithelial-to-mesenchymal transition (EMT), (ii) affecting remodeling of Abbreviations: ACAT, acetoacetyl-CoA transferase; acetyl-CoA, acetyl-coenzyme A; Arp2/3, actin-related protein 2/3 (actin polymerizing complex) BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4. 0/). 14 W. Likus et al. / Drug Resistance Updates 25 (2016) 13-25 the cytoskeleton as well as cell motility, (iii) affecting cell polarity (non-canonical Wnt/planar pathway), and (iv) modulation of mesenchymal-to-epithelial transition (MET). Herein we provide an overview of the mevalonate signaling network. We then briefly highlight diverse functions of various elements of this mevalonate pathway. We further discuss in detail the role of elements of the mevalonate cascade in stemness, carcinogenesis, cancer progression, metastasis and maintenance of cancer stem cells.

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“…Plantago ovata Forssk., belonging to the Plantaginaceae family, is an annual or a perennial subscaulescent herb, up to 14 cm tall, sparsely or thickly covered with soft hairs, with rosette and linear-lanceolate leaves, and it is now as a medicinal herb cultivated widely in West Asia for seed husk known as Isabgol or blonde Psyllium (KAZMI, 1974;KOTWAL et al, 2013). This species has a blend of the two pollinating systems (cross-pollinated and self-pollinated) and has the lowest chromosome number 2n=8 in genus Plantago (KOTWAL et al, 2013;SHARMA et al, 1992). P. ovata belongs to Plantago subgen.…”

Section: Introductionmentioning

confidence: 99%

“…Different molecular markers have been used for genetic diversity study in P. ovata without determining variety, for example, KASWAN et al (2013) worked the genetic diversity of 24 genotypes P. ovata by using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR). Similarly, the genetic relationships between different populations of P. ovata was studied by using random amplified polymorphic DNA (RAPD) (SINGH et al, 2009;KOUR et al, 2016) and amplified fragment length polymorphisms (AFLP) and simple sequence repeats (SSR) (KOTWAL et al, 2013;KUMAR et al, 2014). All these studies focused on genetic variation, and there is no report on its population genetic structure, although such information is necessary for the formulation of effective conservation strategies for this important economic species.…”

Section: Introductionmentioning

confidence: 99%

Population genetic structure in Plantago ovata var. decumbens with using ISSR markers

Mohsenzadeh¹,

Sheidai²,

Koohdar³

2019

Genetika

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Plantago ovata (Plantaginaceae) is a medicinal plant cultivated extensively in Western Asia for seed husk known as blonde Psyllium or Isabgol. We used ISSR molecular markers to investigate the population genetic structure and genetic divergence within P. ovata populations. We identified P. ovata variety as var. decumbens based on the morphological data. WARD tree and PCoA plot produced similar results on morphological data. AMOVA revealed a significant genetic differentiation among the studied populations. STRUCTURE analyses showed some degree of gene flow among the studied populations. NJ tree and PCoA plot of ISSR data revealed that there are at least three genetic groups within the studied populations. Though the studied populations in P. ovata are genetically differentiated, there are not qualitative morphological features for differentiating among theirs. Therefore, we consider them as ecotypes within P. ovata.

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Molecular markers unravel intraspecific and interspecific genetic variability in Plantago ovata and some of its wild allies

Cited by 6 publications

References 16 publications

De Novo Transcriptome Analysis of Medicinally Important Plantago ovata Using RNA-Seq

De Novo Transcriptome Analysis of Medicinally Important Plantago ovata Using RNA-Seq

Could drugs inhibiting the mevalonate pathway also target cancer stem cells?

Population genetic structure in Plantago ovata var. decumbens with using ISSR markers

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