Molecular mapping of the reverse thermo-sensitive genic male-sterile gene (rtms1) in rice

Jia, Jing; Zhang, D. S.; Li, C. Y.; Qu, Xiaoqing; Wang, S. W.; Chamarerk, Varapong; Nguyen, Henry T.; Wang, B.

doi:10.1007/pl00002916

Cited by 59 publications

(43 citation statements)

References 15 publications

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“…Localization studies showed that the TGMS genes from different sources may be located in different chromosomes. Examples include reports that tms1 (Wang et al, 1995), tms2 (Lopez et al, 2003), tms3 (Subudhi et al, 1997), tms4 (Dong et al, 2000), tms5 (Yang et al, 2007a), rtms1 (Jia et al, 2001), ms-h (Koh et al, 1999), and tms6 (Lee et al, 2005) were located on chromosomes 8, 7, 6, 2, 2, 10, 9, and 5, respectively. However, these TGMS materials are not to be applied in rice production because of the non-stability of their sterility.…”

Section: Genetic Diversity Of Ptgms Ricementioning

confidence: 99%

Fine mapping and candidate gene prediction of the photoperiod and thermo-sensitive genic male sterile gene pms1(t) in rice

Zhou

Xianyin

Xue

2011

J. Zhejiang Univ. Sci. B

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Pei′ai64S, an indica sterile variety with photoperiod and thermo-sensitive genic male sterile (PTGMS) genes, has been widely exploited for commercial seed production for "two-line" hybrid rice in China. One PTGMS gene from Pei′ai64S, pms1(t), was mapped by a strategy of bulked-extreme and recessive-class approach with simple sequence repeat (SSR) and insert and deletion (In-Del) markers. Using linkage analysis for the F 2 mapping population consisting of 320 completely male sterile individuals derived from a cross between Pei′ai64S and 93-11 (indica restorer) lines, the pms1(t) gene was delimited to the region between the RM21242 (0.2 cM) and YF11 (0.2 cM) markers on the short arm of chromosome 7. The interval containing the pms1(t) locus, which was co-segregated with RM6776, is a 101.1 kb region based on the Nipponbare rice genome. Fourteen predicted loci were found in this region by the Institute for Genomic Research (TIGR) Genomic Annotation. Based on the function of the locus LOC_Os07g12130 by bioinformatics analysis, it is predicted to encode a protein containing a Myb-like DNA-binding domain, and may process the transcript with thermosensory response. The reverse transcription-polymerase chain reaction (RT-PCR) results revealed that the mRNA levels of LOC_Os07g12130 were altered in different photoperiod and temperature treatments. Thus, the LOC_Os07g12130 locus is the most likely candidate gene for pms1(t). These results may facilitate not only using the molecular marker assisted selection of PTGMS genes, but also cloning of the pms1(t) gene itself.

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Section: Genetic Diversity Of Ptgms Ricementioning

confidence: 99%

Fine mapping and candidate gene prediction of the photoperiod and thermo-sensitive genic male sterile gene pms1(t) in rice

Zhou

Xianyin

Xue

2011

J. Zhejiang Univ. Sci. B

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“…To date, a number of loci that control PGMS or TGMS, including reverse PGMS or reverse TGMS, in different rice lines have been mapped to different chromosomes: photoperiod-sensitive genic male sterile genes pms1 [15,16] [36]. Despite the wide application of PGMS and TGMS lines in hybrid rice breeding, the genes responsible for PGMS and TGMS in these lines have not been cloned, and the molecular mechanisms underlying these traits remain unclear.…”

Section: Introductionmentioning

confidence: 99%

“…To date, a number of loci that control PGMS or TGMS, including reverse PGMS or reverse TGMS, in different rice lines have been mapped to different chromosomes: photoperiod-sensitive genic male sterile genes pms1 [15,16], pms2 [15], pms3 [17][18][19][20]; reverse photoperiod-sensitive genic male sterile genes rpms1 [21], rpms2 [21]; thermo-sensitive genic male-sterile genes tms1 [22], tms2 [23,24], tms3 [25,26], tms4 [27], tms5 [5,[28][29][30][31], tms6 [32], tms6(t) [33]; photoperiod-thermosensitive genic male sterile genes ptgms2-1 [34], pms1(t) [35]; and reverse thermo-sensitive genic male-sterile genes rtms1 [36]. Despite the wide application of PGMS and TGMS lines in hybrid rice breeding, the genes responsible for PGMS and TGMS in these lines have not been cloned, and the molecular mechanisms underlying these traits remain unclear.…”

Section: Introductionmentioning

confidence: 99%

“…However, the male sterilityfertility conversion of PA64S and other indica lines derived from NK58S is controlled mainly by temperature rather than by day length: PA64S exhibits male sterility at temperatures higher than 23.5 °C during the anther development, but it converts to male fertility when the temperature is ~21-23 °C [13,14]. Long-day (14 h) conditions can suppress the degree of sterility-to-fertility conversion under low temperatures (21-23 °C), but shortday (12 h) conditions cannot restore male fertility under high temperatures [14].To date, a number of loci that control PGMS or TGMS, including reverse PGMS or reverse TGMS, in different rice lines have been mapped to different chromosomes: photoperiod-sensitive genic male sterile genes pms1 [15,16] [36]. Despite the wide application of PGMS and TGMS lines in hybrid rice breeding, the genes responsible for PGMS and TGMS in these lines have not been cloned, and the molecular mechanisms underlying these traits remain unclear.…”

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confidence: 99%

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Photoperiod- and thermo-sensitive genic male sterility in rice are caused by a point mutation in a novel noncoding RNA that produces a small RNA

et al. 2012

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Photoperiod-and thermo-sensitive genic male sterility (PGMS and TGMS) are the core components for hybrid breeding in crops. Hybrid rice based on the two-line system using PGMS and TGMS lines has been successfully developed and applied widely in agriculture. However, the molecular mechanism underlying the control of PGMS and TGMS remains obscure. In this study, we mapped and cloned a major locus, p/tms12-1 (photo-or thermo-sensitive genic male sterility locus on chromosome 12), which confers PGMS in the japonica rice line Nongken 58S (NK58S) and TGMS in the indica rice line Peiai 64S (PA64S, derived from NK58S). A 2.4-kb DNA fragment containing the wild-type allele P/TMS12-1 was able to restore the pollen fertility of NK58S and PA64S plants in genetic complementation. P/TMS12-1 encodes a unique noncoding RNA, which produces a 21-nucleotide small RNA that we named osa-smR5864w. A substitution of C-to-G in p/tms12-1, the only polymorphism relative to P/TMS12-1, is present in the mutant small RNA, namely osa-smR5864m. Furthermore, overexpression of a 375-bp sequence of P/TMS12-1 in transgenic NK58S and PA64S plants also produced osa-smR5864w and restored pollen fertility. The small RNA was expressed preferentially in young panicles, but its expression was not markedly affected by different day lengths or temperatures. Our results reveal that the point mutation in p/tms12-1, which probably leads to a loss-of-function for osa-smR5864m, constitutes a common cause for PGMS and TGMS in the japonica and indica lines, respectively. Our findings thus suggest that this noncoding small RNA gene is an important regulator of male development controlled by cross-talk between the genetic networks and environmental conditions. Keywords: rice; PGMS; TGMS; noncoding RNA; small RNA Cell Research (2012) IntroductionAsian cultivated rice (Oryza sativa L.) is one of the world's most important crops, meeting the stable food demand of more than half of the global population. The development of hybrid rice is a major approach for increasing the yield potential of rice, since hybrid rice varieties have about 20% or more yield advantage over improved inbred varieties [1]. Hybrid rice technologies are mainly based on the three-line and two-line systems. The three-line system uses cytoplasmic male sterility lines, maintainer lines and restorer lines [2,3]. The twoline hybrid rice is based on the discovery and application of environmentally sensitive genic male sterile (EGMS) lines, which serve as both the male sterile lines and maintainer lines under different environmental conditions [4]. Thus, the two-line hybrid rice system is an important innovation for the better exploitation of hybrid vigor (heterosis). Compared with the three-line system, the advantages of the two-line system include a wider range Rice PGMS and TGMS caused by a point mutation in a noncoding RNA 650 of germplasm resources used as breeding parents, better grain quality and higher yields, and benefiting from simpler procedures for breeding and hybrid seed production [5...

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“…These are tms1 of 5460S line from China (Wang et al 1995), tms2 of Norin PL12 from Japan (Yamagushi et al 1997), tms3(t) of IR32364TGMS line from IRRI (Subudhi et al 1997), tms4-1 of TGMS-VN1 line from Vietnam (Dong et al 2000), tms4-2 (Reddy et al 2000), tms5 and tms6-1 of AnnongS-1 line from China , tms6-2 of Sokcho-MS line from Korea (Lee at al. 2005), rtms1 of J207S line (Jia et al 2001), and ms-h of Hwacheong ms-h line (Koh et al 1999). Each was localized on chromosome 8, 7, 6, 2, 2, 10 and 9, respectively.…”

Section: Male Sterility In Ricementioning

confidence: 99%

Bioengineering of Male Sterility in Rice (Oryza sativa L.)

Tien¹,

Oo²,

Soh³

et al. 2013

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT Male sterility is an important trait for crop breeding program based on heterosis. Recent advances in molecular researches have led to the identification of genes involved in plant reproductive development and understanding the molecular functions of rice male gametophyte including roles of phytohormones in reproduction process. Here, we review the genes required for key aspects of anther/pollen development and conventional methods for the production of hybrid seeds in rice. Finally, we discuss the molecular approaches for the generation of male-sterile lines through the regulation of phytohormonal biosynthesis in reproductive organs.

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Molecular mapping of the reverse thermo-sensitive genic male-sterile gene (rtms1) in rice

Cited by 59 publications

References 15 publications

Fine mapping and candidate gene prediction of the photoperiod and thermo-sensitive genic male sterile gene pms1(t) in rice

Fine mapping and candidate gene prediction of the photoperiod and thermo-sensitive genic male sterile gene pms1(t) in rice

Photoperiod- and thermo-sensitive genic male sterility in rice are caused by a point mutation in a novel noncoding RNA that produces a small RNA

Bioengineering of Male Sterility in Rice (Oryza sativa L.)

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