Fine mapping and candidate gene prediction of the photoperiod and thermo-sensitive genic male sterile gene pms1(t) in rice

Zhou, Yuanfei; Xianyin, Zhang; Xue, Quan

doi:10.1631/jzus.b1000306

Cited by 18 publications

(21 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Distribution of pollen sterility for the F 2 populations was stable across years that fit a twoor three-gene recessive model (Table 1, Supplemental Files 5-7). The inheritance pattern detected in our research is consistent with different genetic populations in southern China involving PTGMS parents Nongken 58S, Pei'i64S, and others (Zhang et al, 1994;Subudhi et al, 1997;Dong et al, 2000;Zhou et al, 2011Zhou et al, , 2012. Taken together, our studies suggest that male sterility from PTGMS 2008S is governed primarily by two or three recessive genes whose expression is generally stable across different genetic backgrounds when planted in April in southern Louisiana.…”

Section: Discussionsupporting

confidence: 85%

“…Other unknown environmental factors or genetic background may have also contributed to variation observed between the two sites. A second major locus pms1(t) at LOC_Os07g12310 was mapped in crosses with male sterile Pei'ai64S that exhibited altered responses in different photoperiod and temperature regimes (Zhou et al, 2011). Pollen data were not collected and panicles were not bagged prior to flowering in Arkansas.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic Analysis of Photoperiod/Thermosensitive Male Sterility in Rice under US Environments

et al. 2017

View full text Add to dashboard Cite

Two‐line hybrid rice (Oryza sativa L.) breeding in the United States and China uses photoperiod/thermosensitive genetic male sterility (PTGMS) to produce lines with fertile or sterile pollen, depending on temperature and/or daylength. Although studied in China for >30 yr, genetic analysis of PTGMS in US environments is lacking. We therefore conducted genetic studies of male sterility over 3 yr in five F2 and BC1F2 populations derived from PTGMS line 2008S in Louisiana and Arkansas. Chi‐squared analyses in all populations indicated that sterility was controlled at both locations by two or three recessive genes. Inheritance and expression of PTGMS was found to be influenced by the particular combination of population and year (environment). Sequence comparisons of two regions in PTGMS loci LOC_Os07g12130 and LOC_Os12g36030 from six fertile and sterile lines revealed a diagnostic single‐nucleotide polymorphism (SNP) at each locus. Two‐way ANOVA in two F2 populations inferred that interaction of the two SNP‐containing regions contributed up to 65% variation for sterility in one Louisiana environment. Selective genotyping of the 2008S/CL131 F2 population detected new quantitative trait loci with R2 values ranging from 5 to 27% using single‐marker and composite‐interval mapping. Results from this study indicate that 2008S can be used as parental material for development of two‐line hybrids in Louisiana and Arkansas, but extensive testing of populations across years and locations will be required.

show abstract

Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Genetic Analysis of Photoperiod/Thermosensitive Male Sterility in Rice under US Environments

et al. 2017

View full text Add to dashboard Cite

show abstract

“…To date, a number of loci that control PGMS or TGMS, including reverse PGMS or reverse TGMS, in different rice lines have been mapped to different chromosomes: photoperiod-sensitive genic male sterile genes pms1 [15,16], pms2 [15], pms3 [17][18][19][20]; reverse photoperiod-sensitive genic male sterile genes rpms1 [21], rpms2 [21]; thermo-sensitive genic male-sterile genes tms1 [22], tms2 [23,24], tms3 [25,26], tms4 [27], tms5 [5,[28][29][30][31], tms6 [32], tms6(t) [33]; photoperiod-thermosensitive genic male sterile genes ptgms2-1 [34], pms1(t) [35]; and reverse thermo-sensitive genic male-sterile genes rtms1 [36]. Despite the wide application of PGMS and TGMS lines in hybrid rice breeding, the genes responsible for PGMS and TGMS in these lines have not been cloned, and the molecular mechanisms underlying these traits remain unclear.…”

Section: Introductionmentioning

confidence: 99%

Photoperiod- and thermo-sensitive genic male sterility in rice are caused by a point mutation in a novel noncoding RNA that produces a small RNA

et al. 2012

View full text Add to dashboard Cite

Photoperiod-and thermo-sensitive genic male sterility (PGMS and TGMS) are the core components for hybrid breeding in crops. Hybrid rice based on the two-line system using PGMS and TGMS lines has been successfully developed and applied widely in agriculture. However, the molecular mechanism underlying the control of PGMS and TGMS remains obscure. In this study, we mapped and cloned a major locus, p/tms12-1 (photo-or thermo-sensitive genic male sterility locus on chromosome 12), which confers PGMS in the japonica rice line Nongken 58S (NK58S) and TGMS in the indica rice line Peiai 64S (PA64S, derived from NK58S). A 2.4-kb DNA fragment containing the wild-type allele P/TMS12-1 was able to restore the pollen fertility of NK58S and PA64S plants in genetic complementation. P/TMS12-1 encodes a unique noncoding RNA, which produces a 21-nucleotide small RNA that we named osa-smR5864w. A substitution of C-to-G in p/tms12-1, the only polymorphism relative to P/TMS12-1, is present in the mutant small RNA, namely osa-smR5864m. Furthermore, overexpression of a 375-bp sequence of P/TMS12-1 in transgenic NK58S and PA64S plants also produced osa-smR5864w and restored pollen fertility. The small RNA was expressed preferentially in young panicles, but its expression was not markedly affected by different day lengths or temperatures. Our results reveal that the point mutation in p/tms12-1, which probably leads to a loss-of-function for osa-smR5864m, constitutes a common cause for PGMS and TGMS in the japonica and indica lines, respectively. Our findings thus suggest that this noncoding small RNA gene is an important regulator of male development controlled by cross-talk between the genetic networks and environmental conditions. Keywords: rice; PGMS; TGMS; noncoding RNA; small RNA Cell Research (2012) IntroductionAsian cultivated rice (Oryza sativa L.) is one of the world's most important crops, meeting the stable food demand of more than half of the global population. The development of hybrid rice is a major approach for increasing the yield potential of rice, since hybrid rice varieties have about 20% or more yield advantage over improved inbred varieties [1]. Hybrid rice technologies are mainly based on the three-line and two-line systems. The three-line system uses cytoplasmic male sterility lines, maintainer lines and restorer lines [2,3]. The twoline hybrid rice is based on the discovery and application of environmentally sensitive genic male sterile (EGMS) lines, which serve as both the male sterile lines and maintainer lines under different environmental conditions [4]. Thus, the two-line hybrid rice system is an important innovation for the better exploitation of hybrid vigor (heterosis). Compared with the three-line system, the advantages of the two-line system include a wider range Rice PGMS and TGMS caused by a point mutation in a noncoding RNA 650 of germplasm resources used as breeding parents, better grain quality and higher yields, and benefiting from simpler procedures for breeding and hybrid seed production [5...

show abstract

“…) and pms1(t) (Zhou et al. ); reverse photosensitive genic male‐sterile genes: rpms1 and rpms2 (Peng et al. ).…”

mentioning

confidence: 99%

“…Pms1(t) , which was originated from Peiai64S, was mapped on a 101.1‐Kb DNA fragment between markers RM21242 and YF11 on chromosome 7, and the LOC_Os07g12130 locus was most likely the candidate (Zhou et al. ). Although numbers of thermosensitive and photothermosensitive genic male‐sterile genes of rice have been mapped, so far, none of them has been isolated, let alone the sterility molecular mechanism research (Peng et al.…”

mentioning

confidence: 99%

Genetic analysis and fine mapping of tms9, a novel thermosensitive genic male‐sterile gene in rice (Oryza sativa L.)

et al. 2013

View full text Add to dashboard Cite

Two‐line hybrid rice as a novel hybrid breeding method has huge potential for yield increasing and utilization of intersubspecific heterosis, and it is of major significance for the food security of rice‐consuming populations. Zhu1S is a thermosensitive genic male‐sterile line of rice with low critical temperature and excellent combining ability, which has been widely exploited as a female parent in Chinese two‐line hybrid rice breeding. Here, genetic mapping in F2 populations was used to show that its male sterility is inherited as a single recessive gene and that responsible gene (termed tms9) lies on the short arm of chromosome 2. A high‐resolution linkage analysis which was based on the Zhu1S/R173 F2 population found that the thermosensitive genic male‐sterile gene tms9 of Zhu1S was fine mapped between insertion–deletion (Indel) markers Indel 37 and Indel 57, and the genetic distance from the tms9 to the two markers was 0.12 and 0.31 cM, respectively. The physical distance between the two markers was about 107.2 kb. Sequence annotation databases showed that the two Indel markers (Indel 37 and Indel 57) were located on two BAC clones (B1307A11 and P0027A02). There are sixteen open reading frames (ORF) present in this region. The results of this study are of great significance for further cloning tms9 and molecular marker–assisted selection.

show abstract

Fine mapping and candidate gene prediction of the photoperiod and thermo-sensitive genic male sterile gene pms1(t) in rice

Cited by 18 publications

References 35 publications

Genetic Analysis of Photoperiod/Thermosensitive Male Sterility in Rice under US Environments

Genetic Analysis of Photoperiod/Thermosensitive Male Sterility in Rice under US Environments

Photoperiod- and thermo-sensitive genic male sterility in rice are caused by a point mutation in a novel noncoding RNA that produces a small RNA

Genetic analysis and fine mapping of tms9, a novel thermosensitive genic male‐sterile gene in rice (Oryza sativa L.)

Contact Info

Product

Resources

About